Alignments for a candidate for pta in Pseudomonas fluorescens FW300-N2C3

GapMind for catabolism of small carbon sources

Alignments for a candidate for pta in Pseudomonas fluorescens FW300-N2C3

Align Phosphate acetyltransferase; Phosphotransacetylase; EC 2.3.1.8 (characterized)
to candidate AO356_16680 AO356_16680 phosphate acetyltransferase

Query= SwissProt::Q9I5A5
         (704 letters)



>FitnessBrowser__pseudo5_N2C3_1:AO356_16680
          Length = 699

 Score = 1066 bits (2758), Expect = 0.0
 Identities = 547/699 (78%), Positives = 614/699 (87%), Gaps = 8/699 (1%)

Query: 1   MHTFFIAPTGFGVGLTSISLGLLRALERAGLKVGFFKPIAQLHPGDLGPERSSELVARTH 60
           M TFFIAPT FGVGLTSISLGL+R LERAGLKVGFFKPIAQ HPGD GPERS+ELVARTH
Sbjct: 1   MQTFFIAPTDFGVGLTSISLGLVRTLERAGLKVGFFKPIAQPHPGDTGPERSTELVARTH 60

Query: 61  GLDTPKPLPLAQVERMLGDGQLDELLEEIISLYQRAAADKDVVIVEGMVPTRHASYAARV 120
           GL  P+PL LA VERMLGDGQLDELLEEII+LYQ+AA  KDV++VEGMVPTR+ASYAARV
Sbjct: 61  GLKPPQPLGLAHVERMLGDGQLDELLEEIITLYQQAAIGKDVLVVEGMVPTRNASYAARV 120

Query: 121 NFHLAKSLDAEVILVSAPENETLTELTDRIEIQAQLFGGPRDPKVLGVILNKVRGEADAA 180
           N HLAKSLDAEVILVSAPENE LTEL+ R+E+QAQLFGGP+DPKVLGVILNKVR      
Sbjct: 121 NLHLAKSLDAEVILVSAPENEVLTELSGRVELQAQLFGGPKDPKVLGVILNKVR------ 174

Query: 181 NAEDGVADFARRLTEHSPLLRD-DFRLIGCIPWQDELNAARTRDIADLLSARVINAGDYE 239
             E+ +  F+ RL EHSPLLR  DFRL+GCIP++ ELNA RTRD+ADL+ A+V+NAGDYE
Sbjct: 175 -TEESMEAFSARLKEHSPLLRSGDFRLLGCIPFRPELNAPRTRDVADLMGAQVLNAGDYE 233

Query: 240 QRRVQKIVLCARAVPNTVQLLKPGVLVVTPGDRDDIILAASLAAMNGVPLAGLLLCSDFP 299
            RR+  I++CAR + NTV+LLKPGVLVVTPGDRDDIILA SLAA+NGVPLAGLLL SD  
Sbjct: 234 TRRMSNIIICARTMRNTVELLKPGVLVVTPGDRDDIILAVSLAAINGVPLAGLLLTSDTL 293

Query: 300 PDPRIMELCRGALQGGLPVLSVATGSYDTATNLNRMNKEIPVDDRERAERVTEFVAGHID 359
           PDPRIM+LCRGALQ GLPVLSV+TGSYDTA  LN +NKEIP+DDRERAE +T+FVA H+D
Sbjct: 294 PDPRIMDLCRGALQAGLPVLSVSTGSYDTANLLNGLNKEIPIDDRERAEIITDFVASHLD 353

Query: 360 FEWLKQRCGTPRELRLSPPAFRYQVVQRAQKAGKRIVLPEGSEPRTVQAAAICQARGIAR 419
            +WL QRCGTPRE+RLSP  FRYQ++QRAQ A KRIVLPEGSEP TVQAAAICQARGIAR
Sbjct: 354 AKWLHQRCGTPREMRLSPAVFRYQLIQRAQAANKRIVLPEGSEPLTVQAAAICQARGIAR 413

Query: 420 CVLLAKPEEVQAVAQAQGIVLPEGLEIIDPDLVRQRYVEPMVELRKGKGLNAPMAEQQLE 479
           CVLLAKP +V+AVA+AQGI LP GLEI+DPD +R+RYVEPMV LRK K LNAPMAEQQLE
Sbjct: 414 CVLLAKPADVEAVARAQGIELPPGLEILDPDSIRERYVEPMVRLRKSKSLNAPMAEQQLE 473

Query: 480 DSVVLATMMLALDEVDGLVSGAIHTTASTIRPALQLIKTAPGYNLVSSVFFMLLPDQVLV 539
           D+VV+ TMMLALDEVDGLVSG IH+TA+TIRPALQLIKTAPG +LVSSVFFML P++VLV
Sbjct: 474 DTVVIGTMMLALDEVDGLVSGVIHSTANTIRPALQLIKTAPGCSLVSSVFFMLFPEEVLV 533

Query: 540 YGDCAVNPDPSASDLAEIAVQSAASAQAFGIPARVAMISYSTGDSGSGVDVDKVREATRL 599
           YGDC +NP PSA++LAEIA+QSA SA AFGI  RVAM+SYS+G+S SG +V+KVREAT L
Sbjct: 534 YGDCVMNPHPSAAELAEIALQSADSAAAFGITPRVAMLSYSSGESASGEEVEKVREATLL 593

Query: 600 AREQRPDLLIDGPLQYDAAAIASVGRQKAPNSPVAGQATVFIFPDLNTGNTTYKAVQRSA 659
           A E +  LLIDGPLQYDAAA  +V RQ APNS VAG+ATVFIFPDLNTGNTT+KAVQRSA
Sbjct: 594 AHEAQQGLLIDGPLQYDAAANETVARQLAPNSQVAGRATVFIFPDLNTGNTTHKAVQRSA 653

Query: 660 DCVSVGPMLQGLRKPVNDLSRGALVEDIVYTIALTAIQA 698
           DCVS+GPMLQGLRKPVNDL RGA V+DIVYTIALTAIQA
Sbjct: 654 DCVSLGPMLQGLRKPVNDLPRGAQVDDIVYTIALTAIQA 692


Lambda     K      H
   0.319    0.136    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1394
Number of extensions: 57
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 704
Length of database: 699
Length adjustment: 39
Effective length of query: 665
Effective length of database: 660
Effective search space:   438900
Effective search space used:   438900
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)

Align candidate AO356_16680 AO356_16680 (phosphate acetyltransferase)
to HMM TIGR00651 (pta: phosphate acetyltransferase (EC 2.3.1.8))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR00651.hmm
# target sequence database:        /tmp/gapView.26954.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00651  [M=304]
Accession:   TIGR00651
Description: pta: phosphate acetyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                       Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                       -----------
     7e-118  379.5   0.0   9.5e-118  379.0   0.0    1.2  1  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_16680  AO356_16680 phosphate acetyltran


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_16680  AO356_16680 phosphate acetyltransferase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  379.0   0.0  9.5e-118  9.5e-118       1     304 []     389     689 ..     389     689 .. 0.98

  Alignments for each domain:
  == domain 1  score: 379.0 bits;  conditional E-value: 9.5e-118
                                       TIGR00651   1 ivlPEgseervlkAaallaekkiaekvllvnkeeevkn.kakevnlklgkvvvedpdvskdie 62 
                                                     ivlPEgse+ +++Aaa+++ ++ia++vll++ ++++++ +a++++l  g  ++ dpd+   +e
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_16680 389 IVLPEGSEPLTVQAAAICQARGIARCVLLAKPADVEAVaRAQGIELPPGL-EILDPDSI--RE 448
                                                     8************************************9789999998875.56666666..6* PP

                                       TIGR00651  63 kyverlyekrkhkGvtekeareqlrDevslaallvelgeadglvsGavsttaktlrpalqiik 125
                                                     +yve ++ +rk k ++  +a++ql+D+v++++++++l+e+dglvsG  ++ta+t+rpalq+ik
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_16680 449 RYVEPMVRLRKSKSLNAPMAEQQLEDTVVIGTMMLALDEVDGLVSGVIHSTANTIRPALQLIK 511
                                                     *************************************************************** PP

                                       TIGR00651 126 tlegvklvssvfimekeeevlvfaDCavavdPnaeeLAeiAlqsaksakslgeeepkvallsy 188
                                                     t++g +lvssvf+m  +eevlv++DC ++++P+a eLAeiAlqsa+sa ++g + p+va+lsy
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_16680 512 TAPGCSLVSSVFFMLFPEEVLVYGDCVMNPHPSAAELAEIALQSADSAAAFG-ITPRVAMLSY 573
                                                     ****************************************************.********** PP

                                       TIGR00651 189 stkgsgkgeevekvkeAvkilkekepdllldGelqfDaAlvekvaekkapesevagkanvfvF 251
                                                     s++ s++geevekv+eA+ +++e +  ll+dG+lq+DaA  e+va++ ap+s+vag+a+vf+F
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_16680 574 SSGESASGEEVEKVREATLLAHEAQQGLLIDGPLQYDAAANETVARQLAPNSQVAGRATVFIF 636
                                                     *************************************************************** PP

                                       TIGR00651 252 PdLdaGnigYkivqRladaeaiGPilqGlakPvnDLsRGasvedivnvviita 304
                                                     PdL++Gn++ k+vqR+ad  ++GP+lqGl+kPvnDL RGa+v+div+++++ta
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_16680 637 PDLNTGNTTHKAVQRSADCVSLGPMLQGLRKPVNDLPRGAQVDDIVYTIALTA 689
                                                     ***************************************************96 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (304 nodes)
Target sequences:                          1  (699 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.00
# Mc/sec: 22.25
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources