Align NADH-dependent aldehyde reductase (EC 1.1.1.77; EC 1.1.1.244; EC 1.1.1.1) (characterized)
to candidate AO356_25665 AO356_25665 3-beta hydroxysteroid dehydrogenase
Query= metacyc::G3O-32155-MONOMER (298 letters) >FitnessBrowser__pseudo5_N2C3_1:AO356_25665 Length = 288 Score = 218 bits (554), Expect = 2e-61 Identities = 121/291 (41%), Positives = 174/291 (59%), Gaps = 11/291 (3%) Query: 1 MKVFITGASGFIGSAVLSELISSGHEVVGLARSDEAAAKIKSIDPAAKILRGDLKDLEIL 60 M VF+TGA+G++G++V+ +LI++GH V GLAR +E A + + AK++ G L DLEIL Sbjct: 1 MHVFVTGATGWVGASVVQDLIAAGHRVTGLARCEEKGATLAAT--GAKVIPGTLDDLEIL 58 Query: 61 KKGATESDGVIHLGFVHDFKNFEQCCEIDRQATVAMLESLKGSNKPFLYTNGTLSLRPNK 120 A+ +D VIH F HDF F + + DR+A + ++L+GS++P L T+G L + Sbjct: 59 HSAASAADAVIHTAFNHDFSRFAENAQQDRRAIETLGDALRGSDRPLLVTSGLFGL--TR 116 Query: 121 VANEQDGIDEDSKILRAVTEQVALSYKDKGVSARIVRLPFSVHGKGDKAFVPILMNIAKA 180 A+E + + S +E A ++G VRL SVHG GD FVPIL+ +A+ Sbjct: 117 GASELEVVSPASP---RKSEAAARDLAERGGRVATVRLAPSVHGLGDHGFVPILIRLARQ 173 Query: 181 AGKSGYVGQGTNAWAAVHRLDTAPLFRLVLEKGKTGQVYHCVGEQGIPFKDIARVIGEIL 240 +G S Y G G++ W+ VHR D A ++RL LE+G T VYH V ++ +PFKDIA+ IG L Sbjct: 174 SGVSAYPGDGSHCWSGVHRQDAARVYRLALEQGVTQSVYHAVADEAVPFKDIAQAIGRGL 233 Query: 241 NVPVASIPVDDAESHFGFLTCFVTRDGPVSSEGTRKELGWQPQQIGLLEDI 291 +PV S HFG+ + VSSE TR LGW P GLL+D+ Sbjct: 234 GLPVES----REREHFGWFAHMAGANMAVSSEYTRALLGWTPSGPGLLDDL 280 Lambda K H 0.318 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 242 Number of extensions: 11 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 298 Length of database: 288 Length adjustment: 26 Effective length of query: 272 Effective length of database: 262 Effective search space: 71264 Effective search space used: 71264 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory