GapMind for catabolism of small carbon sources

 

Aligments for a candidate for fruG in Pseudomonas fluorescens FW300-N2C3

Align Fructose import permease protein FruG (characterized)
to candidate AO356_00960 AO356_00960 ABC transporter permease

Query= SwissProt::Q8G845
         (340 letters)



>lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_00960 AO356_00960 ABC
           transporter permease
          Length = 325

 Score =  124 bits (312), Expect = 3e-33
 Identities = 87/297 (29%), Positives = 150/297 (50%), Gaps = 12/297 (4%)

Query: 33  ILMIIMGQALFGTYIRLGFISSLFIDHAYLIILAVAMTLPILTGGIDLSVGAIVAITA-V 91
           + M+ +   L   ++     S+L      L++LAV MT  ++ GGIDLSVG+++A+ A  
Sbjct: 28  LAMVALFSTLSSHFLSYDTFSTLANQIPDLMVLAVGMTFILIIGGIDLSVGSVLALAAST 87

Query: 92  VGLKLANAGVPAFLVMIIMLLIGAVFGLLAGTLIEEFNMQPFIATLSTMFLARGLASIIS 151
           V + +   G   +   ++ + + A+ G + G++   + +  FI +L  + +ARGLA  + 
Sbjct: 88  VSVAVLGWGWSVWPSALLGMAVAALAGTVTGSITVAWRIPSFIVSLGVLEMARGLAYQM- 146

Query: 152 TDSLTFPQGNDFSFISNVIKIIDNPKISNDLSFNVGVIIALVVVVFGYVFLHHTRTGRTI 211
           T S T   G+ F+++SN I    +P            IIAL+V+      L  T  GR +
Sbjct: 147 TGSRTAYIGDSFAWLSNPIAFGISPSF----------IIALLVIFIAQAVLTRTVFGRYL 196

Query: 212 YAIGGSRSSAELMGLPVKRTQYIIYLTSATLAALASIVYTANIGSAKNTVGVGWELDAVA 271
             IG +  +  L G+  K  + +++     LA +A++   + + +A    G G EL  +A
Sbjct: 197 IGIGTNEEAVRLAGINPKPYKILVFSLMGLLAGVAALFQISRLEAADPNAGSGLELQVIA 256

Query: 272 SVVIGGTIITGGFGYVLGSVLGSLVRSILDPLTSDFGVPAEWTTIVIGLMILVFVVL 328
           +VVIGGT + GG G V+ +  G L+ S+L    +  G       I+ G +I+V VVL
Sbjct: 257 AVVIGGTSLMGGRGSVISTFFGVLIISVLAAGLAQIGATEPTKRIITGAVIVVAVVL 313



 Score = 25.8 bits (55), Expect = 0.002
 Identities = 33/130 (25%), Positives = 58/130 (44%), Gaps = 10/130 (7%)

Query: 26  LAAVVIFILMIIMGQALFGTYIRLGFISSLFIDHAYLIILAVAMTLPILTGGIDLSVGAI 85
           +A +VIFI   ++ + +FG Y+ +G  ++   + A  +         IL   +   +  +
Sbjct: 175 IALLVIFIAQAVLTRTVFGRYL-IGIGTN---EEAVRLAGINPKPYKILVFSLMGLLAGV 230

Query: 86  VAITAVVGLKLA--NAGVPAFLVMIIMLLIGAVFGLLAGTLIEEFNMQPFIATLSTMFLA 143
            A+  +  L+ A  NAG    L +I  ++IG    L+ G       +  F   L    LA
Sbjct: 231 AALFQISRLEAADPNAGSGLELQVIAAVVIGGT-SLMGG---RGSVISTFFGVLIISVLA 286

Query: 144 RGLASIISTD 153
            GLA I +T+
Sbjct: 287 AGLAQIGATE 296


Lambda     K      H
   0.327    0.142    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 270
Number of extensions: 18
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 340
Length of database: 325
Length adjustment: 28
Effective length of query: 312
Effective length of database: 297
Effective search space:    92664
Effective search space used:    92664
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory