Alignments for a candidate for fruI in Pseudomonas fluorescens FW300-N2C3

GapMind for catabolism of small carbon sources

Alignments for a candidate for fruI in Pseudomonas fluorescens FW300-N2C3

Align Phosphoenolpyruvate--protein phosphotransferase (EC 2.7.3.9) (characterized)
to candidate AO356_10350 AO356_10350 phosphoenolpyruvate-protein phosphotransferase

Query= reanno::WCS417:GFF780
         (952 letters)



>FitnessBrowser__pseudo5_N2C3_1:AO356_10350
          Length = 759

 Score =  333 bits (854), Expect = 3e-95
 Identities = 206/565 (36%), Positives = 325/565 (57%), Gaps = 24/565 (4%)

Query: 404 IAAAPGIAIGPAHIQVLQVFDYPLRGESCAI----ERERLHSALADVRRDIQGLIERSQS 459
           +  +PG A+G A + +L   D  +  +        E     +A+  VR D++ L  +  +
Sbjct: 181 VPGSPGAAVGTA-VVMLPPADLDVVPDKTITDINAELGLFKTAIEGVRADMRALSAKLAT 239

Query: 460 KAI---REIFVTHQEMLDDPELTDEVDTRLKQGESAEAAWMSVIEAAAKQQESLQDALLA 516
           +     R +F  +  MLDD  L  EV T +K G+ A+ A   V+     + E + DA L 
Sbjct: 240 QLRPEERALFDVYLMMLDDASLGSEVTTVIKTGQWAQGALRQVVTDHVNRFELMDDAYLR 299

Query: 517 ERAADLRDIGRRVLAQLCGVETSQEPSEPY----ILVMDEVGPSDVARLDPARVAGILTA 572
           ERA+D++D+GRR+LA L   +  ++ +  Y    ILV +E+ P+ +  +   ++AG+++ 
Sbjct: 300 ERASDVKDLGRRLLAYL---QQERQQTLVYPDNTILVSEELTPAMLGEVPEGKLAGLVSV 356

Query: 573 RGGATAHSAIVARALGIPALVGAGPAVLLLAAGTPLLLDGQRGRLHVD-ADAATLQRATV 631
            G   +H AI+ARA+GIP ++G          G  +++DG  G ++ + +D    Q A V
Sbjct: 357 LGSGNSHVAILARAMGIPTVMGLVDLPYSKVDGIQMIVDGYHGEVYTNPSDVLRKQFADV 416

Query: 632 ERDTREQRLQAASAQRHEPALTRDGHAVEVFANIGESAGVASAVEQGAEGIGLLRTELIF 691
             + ++  L    A R  P +T DGH + ++ N G  A VA A ++GAEG+GL RTE+ F
Sbjct: 417 VEEEKQLSL-GLDALRDLPCVTLDGHRMPLWVNTGLLADVARAQKRGAEGVGLYRTEVPF 475

Query: 692 MAHPQAPDEATQEAEYRRVLDGLAGRPLVVRTLDVGGDKPLPYWPIAEEENPFLGVRGIR 751
           M + + P E  Q A YR  L     +P+ +R+LD+GGDK L Y+PI +E+NPFLG RGIR
Sbjct: 476 MINQRFPSEKEQLAIYREQLAAFHPQPVTMRSLDIGGDKSLSYFPI-KEDNPFLGWRGIR 534

Query: 752 LTLQRPQIMEAQLRALLRSADN-RPLRIMFPMVGSVDEWRAARDMTERLRLEI-----PV 805
           +TL  P+I   Q RA+L++++    LRI+ PM+    E   A  +  R   E+      V
Sbjct: 535 VTLDHPEIFLVQARAMLKASEGLNNLRILLPMISGTHELEEALHLIHRAWGEVRDEGTDV 594

Query: 806 ADLQLGIMIEVPSAALLAPVLAKEVDFFSVGTNDLTQYTLAIDRGHPTLSAQADGLHPAV 865
               +G+MIE+P+A      LA++VDF SVG+NDLTQY LA+DR +P ++   D LHPAV
Sbjct: 595 PMPPVGVMIEIPAAVYQTKELARQVDFLSVGSNDLTQYLLAVDRNNPRVADLYDYLHPAV 654

Query: 866 LQLIDITVRAAHAHGKWVGVCGELAADPLAVPVLVGLGVDELSVSARSIPEVKARVREFS 925
           LQ +   VR AHA GK V +CGE+A DP A  +L+ +G D LS++A ++P+VK  +R+ +
Sbjct: 655 LQALQNVVRDAHAEGKPVSICGEMAGDPAAAVLLMAMGFDSLSMNATNLPKVKWMLRQIN 714

Query: 926 LSEAQGLAQKALAVGSPAEVRALVE 950
           LS+AQ L  + + + +P  + + ++
Sbjct: 715 LSKAQELLAEVMTIDNPQVIHSSLQ 739


Lambda     K      H
   0.318    0.135    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1419
Number of extensions: 71
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 952
Length of database: 759
Length adjustment: 42
Effective length of query: 910
Effective length of database: 717
Effective search space:   652470
Effective search space used:   652470
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources