GapMind for catabolism of small carbon sources

 

L-fucose catabolism in Pseudomonas fluorescens FW300-N2C3

Best path

HSERO_RS05250, HSERO_RS05255, HSERO_RS05260, fucU, fdh, fuconolactonase, fucD, fucDH, KDF-hydrolase

Also see fitness data for the top candidates

Rules

Overview: Fucose degradation in GapMind is based on the MetaCyc pathway via L-fuculose (link) or the oxidative pathway via 2,4-diketo-3-deoxy-L-fuconate (KDF) hydrolase (PMC6336799).

23 steps (13 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
HSERO_RS05250 ABC transporter for L-fucose, ATPase component AO356_00965 AO356_23205
HSERO_RS05255 ABC transporter for L-fucose, permease component AO356_00960 AO356_23210
HSERO_RS05260 ABC transporter for L-fucose, substrate-binding component
fucU L-fucose mutarotase FucU
fdh L-fucose 1-dehydrogenase AO356_29900 AO356_23595
fuconolactonase L-fucono-1,5-lactonase
fucD L-fuconate dehydratase AO356_27460 AO356_24195
fucDH 2-keto-3-deoxy-L-fuconate 4-dehydrogenase AO356_28740 AO356_20240
KDF-hydrolase 2,4-diketo-3-deoxy-L-fuconate hydrolase AO356_28735 AO356_27435
Alternative steps:
aldA lactaldehyde dehydrogenase AO356_26145 AO356_28685
BPHYT_RS34240 ABC transporter for L-fucose, permease component AO356_20255 AO356_00960
BPHYT_RS34245 ABC transporter for L-fucose, ATPase component AO356_00965 AO356_23205
BPHYT_RS34250 ABC transporter for L-fucose, substrate-binding component
fucA L-fuculose-phosphate aldolase FucA
fucI L-fucose isomerase FucI
fucK L-fuculose kinase FucK
fucO L-lactaldehyde reductase AO356_25665 AO356_04765
fucP L-fucose:H+ symporter FucP
SM_b21103 ABC transporter for L-fucose, substrate-binding component
SM_b21104 ABC transporter for L-fucose, permease component 1
SM_b21105 ABC transporter for L-fucose, permease component 2 AO356_27680 AO356_28580
SM_b21106 ABC transporter for L-fucose, ATPase component AO356_00010 AO356_27685
tpi triose-phosphate isomerase AO356_07440 AO356_13690

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory