Alignments for a candidate for garD in Pseudomonas fluorescens FW300-N2C3

GapMind for catabolism of small carbon sources

Alignments for a candidate for garD in Pseudomonas fluorescens FW300-N2C3

Align Galactarate dehydratase (EC 4.2.1.42) (characterized)
to candidate AO356_07170 AO356_07170 galactarate dehydratase

Query= reanno::WCS417:GFF829
         (517 letters)



>FitnessBrowser__pseudo5_N2C3_1:AO356_07170
          Length = 517

 Score =  990 bits (2560), Expect = 0.0
 Identities = 490/517 (94%), Positives = 505/517 (97%)

Query: 1   MQLIEHADSPRSIRLHERDNVVIVVNDQGVPAGTEFPDGLVTVDFIPQSHKVTLEDIPEG 60
           MQLIEHADSPR IRLHERDNVVIVVNDQGVPAGTEFPDGLVTVDF+PQSHKVTL+D+PEG
Sbjct: 1   MQLIEHADSPRYIRLHERDNVVIVVNDQGVPAGTEFPDGLVTVDFVPQSHKVTLQDVPEG 60

Query: 61  GQIIRYGQTIGYALAPIPRGSWVQEDQLRMPTAPPLDSLPLSTEVPEAQAPLEGFTFEGY 120
           G++IRYGQ IGYAL PIPRGSWV+EDQLRMPTAPPLDSLPLST+VP A APLEG+TFEGY
Sbjct: 61  GEVIRYGQVIGYALQPIPRGSWVKEDQLRMPTAPPLDSLPLSTDVPAADAPLEGYTFEGY 120

Query: 121 RNADGTVGTRNILGITTTVQCVTGVLDHAVKRIKDELLPKYPHVDDVVALTHSYGCGVAI 180
           RNADGTVGTRNILGITTTVQCVTGVLDHAVKRIKDELLPKYP+VDDVVALTHSYGCGVAI
Sbjct: 121 RNADGTVGTRNILGITTTVQCVTGVLDHAVKRIKDELLPKYPNVDDVVALTHSYGCGVAI 180

Query: 181 TATDAYIPIRTVRNLARNPNLGGEALVISLGCEKLQAGQVMHDNDSSVDLSEPWLYRLQD 240
           TATDAYIPIRTVRNLARNPNLGGEALVISLGCEKLQAGQVMH+ DSSVDLSEPWLYRLQD
Sbjct: 181 TATDAYIPIRTVRNLARNPNLGGEALVISLGCEKLQAGQVMHEGDSSVDLSEPWLYRLQD 240

Query: 241 SSHGFTEMIEQIMALAETRLKKLDQRRRETVPASELILGMQCGGSDAFSGITANPALGYA 300
           SSHGFTEMIEQIM LAETRLKKLD RRRETVPASELILGMQCGGSDAFSGITANPALGYA
Sbjct: 241 SSHGFTEMIEQIMELAETRLKKLDLRRRETVPASELILGMQCGGSDAFSGITANPALGYA 300

Query: 301 SDLLLRAGATVMFSEVTEVRDAIYLLTSRAENTQVAQELVREMDWYDRYLAKGEADRSAN 360
           SDLLLRAGATVMFSEVTEVRDAIYLLTSRA+  +VAQELVREMDWYDRYLAKGEADRSAN
Sbjct: 301 SDLLLRAGATVMFSEVTEVRDAIYLLTSRAQTQEVAQELVREMDWYDRYLAKGEADRSAN 360

Query: 361 TTPGNKKGGLSNIVEKSLGSIVKSGSSAINGVLGPGERFKGKGLIFCATPASDFVCGTLQ 420
           TTPGNKKGGLSNIVEKSLGSIVKSGSSAINGVLGPGERFK KGLIFCATPASDFVCGTLQ
Sbjct: 361 TTPGNKKGGLSNIVEKSLGSIVKSGSSAINGVLGPGERFKQKGLIFCATPASDFVCGTLQ 420

Query: 421 LAAGMNLHVFTTGRGTPYGLAMAPVVKVSTRTELAQRWPDLIDIDAGRIATGRATIEELG 480
           LAAGMNLHVFTTGRGTPYGLAMAPVVKVSTRTELAQRWPDLIDIDAGRIATGRATIEELG
Sbjct: 421 LAAGMNLHVFTTGRGTPYGLAMAPVVKVSTRTELAQRWPDLIDIDAGRIATGRATIEELG 480

Query: 481 WELFHFYLDVASGRKQTWAEHHKLHNDITLFNPAPIT 517
           WELFH+YLDVASG+KQTWAE HKL+NDITLFNPAPIT
Sbjct: 481 WELFHYYLDVASGKKQTWAERHKLYNDITLFNPAPIT 517


Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 985
Number of extensions: 17
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 517
Length of database: 517
Length adjustment: 35
Effective length of query: 482
Effective length of database: 482
Effective search space:   232324
Effective search space used:   232324
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

Align candidate AO356_07170 AO356_07170 (galactarate dehydratase)
to HMM TIGR03248 (garD: galactarate dehydratase (EC 4.2.1.42))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR03248.hmm
# target sequence database:        /tmp/gapView.11627.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR03248  [M=507]
Accession:   TIGR03248
Description: galactar-dH20: galactarate dehydratase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                       Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                       -----------
   6.1e-296  967.6   0.0   6.9e-296  967.4   0.0    1.0  1  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_07170  AO356_07170 galactarate dehydrat


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_07170  AO356_07170 galactarate dehydratase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  967.4   0.0  6.9e-296  6.9e-296       2     507 .]      12     517 .]      11     517 .] 1.00

  Alignments for each domain:
  == domain 1  score: 967.4 bits;  conditional E-value: 6.9e-296
                                       TIGR03248   2 yirvneqdnvaivvndkGlpagtefedgltlvekipqghkvalvdlekgdaiirygeviGyav 64 
                                                     yir++e+dnv+ivvnd+G+pagtef+dgl++v+ +pq+hkv+l+d+ +g ++iryg+viGya+
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_07170  12 YIRLHERDNVVIVVNDQGVPAGTEFPDGLVTVDFVPQSHKVTLQDVPEGGEVIRYGQVIGYAL 74 
                                                     9************************************************************** PP

                                       TIGR03248  65 kdiarGswvkeellelpsapaleelplatkvpeklapleGytfeGyrnadGsvGtknilgitt 127
                                                     + i+rGswvke++l++p+ap+l++lpl+t vp++ apleGytfeGyrnadG+vGt+nilgitt
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_07170  75 QPIPRGSWVKEDQLRMPTAPPLDSLPLSTDVPAADAPLEGYTFEGYRNADGTVGTRNILGITT 137
                                                     *************************************************************** PP

                                       TIGR03248 128 svqcvagvvdyavkrikkellpkypnvddvvalnhsyGcGvaidapdaivpirtlrnlalnpn 190
                                                     +vqcv+gv+d+avkrik+ellpkypnvddvval+hsyGcGvai+a+da++pirt+rnla+npn
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_07170 138 TVQCVTGVLDHAVKRIKDELLPKYPNVDDVVALTHSYGCGVAITATDAYIPIRTVRNLARNPN 200
                                                     *************************************************************** PP

                                       TIGR03248 191 lGGealvvglGceklqperllpeelsavelkdaavlrlqdeklGfaemveailelaeerlkkl 253
                                                     lGGealv++lGceklq+ ++++e+ s+v+l++++++rlqd+++Gf+em+e+i+elae+rlkkl
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_07170 201 LGGEALVISLGCEKLQAGQVMHEGDSSVDLSEPWLYRLQDSSHGFTEMIEQIMELAETRLKKL 263
                                                     *************************************************************** PP

                                       TIGR03248 254 narkretvpaselvvGlqcGGsdafsGvtanpavGfaadllvraGatvlfsevtevrdaihll 316
                                                     + r+retvpasel++G+qcGGsdafsG+tanpa+G+a+dll+raGatv+fsevtevrdai+ll
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_07170 264 DLRRRETVPASELILGMQCGGSDAFSGITANPALGYASDLLLRAGATVMFSEVTEVRDAIYLL 326
                                                     *************************************************************** PP

                                       TIGR03248 317 tpraedaevakaliremkwydeylarGeadrsanttpGnkkGGlsnivekalGsivksGssai 379
                                                     t+ra+++eva+ l+rem+wyd+yla+GeadrsanttpGnkkGGlsnivek+lGsivksGssai
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_07170 327 TSRAQTQEVAQELVREMDWYDRYLAKGEADRSANTTPGNKKGGLSNIVEKSLGSIVKSGSSAI 389
                                                     *************************************************************** PP

                                       TIGR03248 380 vevlspGekvkkkGliyaatpasdfvcGtlqlasglnlhvfttGrGtpyGlalvpvikvstrt 442
                                                     ++vl+pGe+ k+kGli++atpasdfvcGtlqla+g+nlhvfttGrGtpyGla++pv+kvstrt
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_07170 390 NGVLGPGERFKQKGLIFCATPASDFVCGTLQLAAGMNLHVFTTGRGTPYGLAMAPVVKVSTRT 452
                                                     *************************************************************** PP

                                       TIGR03248 443 elaerwadlidldaGriatGeatiedvGwelfrlildvasGrkktwaekyklhndlalfnpap 505
                                                     ela+rw dlid+daGriatG+atie++Gwelf+ +ldvasG+k+twae++kl+nd++lfnpap
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_07170 453 ELAQRWPDLIDIDAGRIATGRATIEELGWELFHYYLDVASGKKQTWAERHKLYNDITLFNPAP 515
                                                     *************************************************************** PP

                                       TIGR03248 506 vt 507
                                                     +t
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_07170 516 IT 517
                                                     *8 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (507 nodes)
Target sequences:                          1  (517 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.03u 0.01s 00:00:00.04 Elapsed: 00:00:00.03
# Mc/sec: 7.47
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources