GapMind for catabolism of small carbon sources

 

Aligments for a candidate for ptsB in Pseudomonas fluorescens FW300-N2C3

Align IIB aka PtsB, component of N-Acetylglucosamine (NAG) porter (PtsBC1C2)(also may facilitate xylose transport) (characterized)
to candidate AO356_17535 AO356_17535 PTS N-acetyl-D-glucosamine transporter

Query= TCDB::Q8GBT8
         (95 letters)



>lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_17535 AO356_17535 PTS
           N-acetyl-D-glucosamine transporter
          Length = 571

 Score = 59.7 bits (143), Expect = 4e-14
 Identities = 32/72 (44%), Positives = 46/72 (63%), Gaps = 2/72 (2%)

Query: 22  KAEKIVAGLGGIENIEEVEGCITRLRTEVVDASKVDEAALKAAGAHGVVKMGS--AIQVV 79
           +A   +  LGG +N+  +  C TRLR ++VD +K  +A LKA GA  VV+ G+  ++QVV
Sbjct: 385 RAAAYIQALGGADNLITIGACTTRLRLDMVDRNKASDAQLKALGAMAVVRPGNGGSLQVV 444

Query: 80  IGTDADPIAAEI 91
           +G  AD IA EI
Sbjct: 445 VGPMADSIADEI 456


Lambda     K      H
   0.314    0.130    0.357 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 108
Number of extensions: 6
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 95
Length of database: 571
Length adjustment: 22
Effective length of query: 73
Effective length of database: 549
Effective search space:    40077
Effective search space used:    40077
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 45 (21.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory