Align Histidine transport system permease protein HisM (characterized)
to candidate AO356_23505 AO356_23505 amino acid ABC transporter permease
Query= SwissProt::P0A2I7 (235 letters) >FitnessBrowser__pseudo5_N2C3_1:AO356_23505 Length = 233 Score = 260 bits (665), Expect = 1e-74 Identities = 121/231 (52%), Positives = 178/231 (77%) Query: 1 MIEIIQEYWKSLLWTDGYRFTGVAITLWLLISSVVMGGLLAVILAVGRVSSNKFIRFPIW 60 MIEI++E+ + LW+DGYR++G+ +TLWLL++S V+G A+ LA+ R N+++ P+ Sbjct: 1 MIEILKEFGPNFLWSDGYRWSGLVVTLWLLVTSAVIGLCAAIPLALVRTYGNRWLALPVQ 60 Query: 61 LFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTDLLNAFFRSGLNCTVLALTLNTCAYTTEI 120 L+T + RGTPL+VQLL+ YSG+ +L +++ + L FFR G++C +LAL L+TCAYT EI Sbjct: 61 LYTLVLRGTPLFVQLLIIYSGLASLSVIRESPSLWWFFRDGMHCVILALALHTCAYTVEI 120 Query: 121 FAGAIRSVPHGEIEAARAYGFSSFKMYRCIILPSALRIALPAYSNEVILMLHSTALAFTA 180 AG +R+ P GEIEAA A G + +++ +++PS LR ALPAYSNEVI +LH+TA+AFTA Sbjct: 121 LAGVLRTTPRGEIEAALALGMTRTQLFLLVLIPSMLRRALPAYSNEVIFVLHATAIAFTA 180 Query: 181 TVPDLLKIARDINSATYQPFTAFGIAAVLYLLISYVLISLFRRAERRWLQH 231 TVPD+LKIA D+N+AT++ F A+GIAA+LY+L++ L+ +FR AERR L + Sbjct: 181 TVPDILKIAADVNAATFKTFQAYGIAALLYMLLACALVGVFRLAERRLLAY 231 Lambda K H 0.330 0.141 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 214 Number of extensions: 4 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 235 Length of database: 233 Length adjustment: 23 Effective length of query: 212 Effective length of database: 210 Effective search space: 44520 Effective search space used: 44520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory