Alignments for a candidate for hutF in Pseudomonas fluorescens FW300-N2C3

GapMind for catabolism of small carbon sources

Alignments for a candidate for hutF in Pseudomonas fluorescens FW300-N2C3

Align Formiminoglutamic iminohydrolase (EC 3.5.3.13) (characterized)
to candidate AO356_09645 AO356_09645 N-formimino-L-glutamate deiminase

Query= reanno::pseudo5_N2C3_1:AO356_09645
         (454 letters)



>FitnessBrowser__pseudo5_N2C3_1:AO356_09645
          Length = 454

 Score =  923 bits (2385), Expect = 0.0
 Identities = 454/454 (100%), Positives = 454/454 (100%)

Query: 1   MSAFFAERALLPSGWAHDVRLEVDAKGVLTHIQAGSHADGAERLGGPLLPGMPNLHSHAF 60
           MSAFFAERALLPSGWAHDVRLEVDAKGVLTHIQAGSHADGAERLGGPLLPGMPNLHSHAF
Sbjct: 1   MSAFFAERALLPSGWAHDVRLEVDAKGVLTHIQAGSHADGAERLGGPLLPGMPNLHSHAF 60

Query: 61  QRAMAGLAEVAGNPNDSFWTWRDLMYRLVGKISPDQLGIIARQLYIEMLKAGYTSVAEFH 120
           QRAMAGLAEVAGNPNDSFWTWRDLMYRLVGKISPDQLGIIARQLYIEMLKAGYTSVAEFH
Sbjct: 61  QRAMAGLAEVAGNPNDSFWTWRDLMYRLVGKISPDQLGIIARQLYIEMLKAGYTSVAEFH 120

Query: 121 YVHHDTDGAPYADPAELALRISHAASAAGIGLTLLPVLYSHSGFGGQAPNDGQRRFINST 180
           YVHHDTDGAPYADPAELALRISHAASAAGIGLTLLPVLYSHSGFGGQAPNDGQRRFINST
Sbjct: 121 YVHHDTDGAPYADPAELALRISHAASAAGIGLTLLPVLYSHSGFGGQAPNDGQRRFINST 180

Query: 181 ENYLKLQSRLQPILAGQPAQALGLCFHSLRAVTPGQIQDVLAASDRDCPVHIHIAEQQKE 240
           ENYLKLQSRLQPILAGQPAQALGLCFHSLRAVTPGQIQDVLAASDRDCPVHIHIAEQQKE
Sbjct: 181 ENYLKLQSRLQPILAGQPAQALGLCFHSLRAVTPGQIQDVLAASDRDCPVHIHIAEQQKE 240

Query: 241 VDDCLSWSGARPLQWLYENTEVDQRWCLVHATHANAQEVSLMAKSRAIAGLCLTTEANLG 300
           VDDCLSWSGARPLQWLYENTEVDQRWCLVHATHANAQEVSLMAKSRAIAGLCLTTEANLG
Sbjct: 241 VDDCLSWSGARPLQWLYENTEVDQRWCLVHATHANAQEVSLMAKSRAIAGLCLTTEANLG 300

Query: 301 DGIFPAVDFLAQGGRLGIGSDSHVSLSVVEELRWLEYGQRLRDQRRNRLYRADQPMVGRT 360
           DGIFPAVDFLAQGGRLGIGSDSHVSLSVVEELRWLEYGQRLRDQRRNRLYRADQPMVGRT
Sbjct: 301 DGIFPAVDFLAQGGRLGIGSDSHVSLSVVEELRWLEYGQRLRDQRRNRLYRADQPMVGRT 360

Query: 361 LFDAALEGGAQALGQPVGALDIGKRADWLVLDGNDPYLATANGDGILNRWLFAGGDRQVR 420
           LFDAALEGGAQALGQPVGALDIGKRADWLVLDGNDPYLATANGDGILNRWLFAGGDRQVR
Sbjct: 361 LFDAALEGGAQALGQPVGALDIGKRADWLVLDGNDPYLATANGDGILNRWLFAGGDRQVR 420

Query: 421 DVMVGGRWVVRDGRHAGEEESARAFTQVLRELLD 454
           DVMVGGRWVVRDGRHAGEEESARAFTQVLRELLD
Sbjct: 421 DVMVGGRWVVRDGRHAGEEESARAFTQVLRELLD 454


Lambda     K      H
   0.321    0.137    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 961
Number of extensions: 32
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 454
Length of database: 454
Length adjustment: 33
Effective length of query: 421
Effective length of database: 421
Effective search space:   177241
Effective search space used:   177241
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

Align candidate AO356_09645 AO356_09645 (N-formimino-L-glutamate deiminase)
to HMM TIGR02022 (hutF: formiminoglutamate deiminase (EC 3.5.3.13))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR02022.hmm
# target sequence database:        /tmp/gapView.23587.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR02022  [M=455]
Accession:   TIGR02022
Description: hutF: formiminoglutamate deiminase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                       Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                       -----------
   4.7e-227  740.0   0.4   5.2e-227  739.9   0.4    1.0  1  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09645  AO356_09645 N-formimino-L-glutam


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09645  AO356_09645 N-formimino-L-glutamate deiminase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  739.9   0.4  5.2e-227  5.2e-227       1     454 [.       1     453 [.       1     454 [] 0.99

  Alignments for each domain:
  == domain 1  score: 739.9 bits;  conditional E-value: 5.2e-227
                                       TIGR02022   1 akvyfaerallpdgwaegvrlavaedGrilavetgvsaaedaerlsgvvlpglanlHsHAFqr 63 
                                                     ++++faerallp gwa++vrl+v+++G ++ +++g+  a++aerl+g++lpg++nlHsHAFqr
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09645   1 MSAFFAERALLPSGWAHDVRLEVDAKGVLTHIQAGSH-ADGAERLGGPLLPGMPNLHSHAFQR 62 
                                                     5789****************************99998.************************* PP

                                       TIGR02022  64 alaGlaevagsgaDsFWtWRevmYrlverldPeqleaiarqlyvemlkaGftrvgEFHYlHHa 126
                                                     a+aGlaevag+++DsFWtWR++mYrlv +++P+ql +iarqly+emlkaG+t+v+EFHY+HH+
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09645  63 AMAGLAEVAGNPNDSFWTWRDLMYRLVGKISPDQLGIIARQLYIEMLKAGYTSVAEFHYVHHD 125
                                                     *************************************************************** PP

                                       TIGR02022 127 adGtpYadpaelaeriaaAAadaGigltlLpvlYahagFGgaaanegqrrfiddveaflrlve 189
                                                     +dG+pYadpaela ri++AA+ aGigltlLpvlY+h+gFGg+a+n+gqrrfi+++e++l+l++
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09645 126 TDGAPYADPAELALRISHAASAAGIGLTLLPVLYSHSGFGGQAPNDGQRRFINSTENYLKLQS 188
                                                     *************************************************************** PP

                                       TIGR02022 190 alrkelaaqeearlGlaiHslRAvtaeelaavlqaserqlPvHiHvaEqqkEvddClaasgrr 252
                                                     +l++ la+q+++ lGl++HslRAvt+ ++++vl+as+r +PvHiH+aEqqkEvddCl +sg r
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09645 189 RLQPILAGQPAQALGLCFHSLRAVTPGQIQDVLAASDRDCPVHIHIAEQQKEVDDCLSWSGAR 251
                                                     *************************************************************** PP

                                       TIGR02022 253 PvewLldhaevdarwclvHatHltdeevkllaksgavaglCpttEanLgDGifpaadfvaagg 315
                                                     P++wL++++evd+rwclvHatH +++ev+l+aks+a+aglC+ttEanLgDGifpa+df+a+gg
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09645 252 PLQWLYENTEVDQRWCLVHATHANAQEVSLMAKSRAIAGLCLTTEANLGDGIFPAVDFLAQGG 314
                                                     *************************************************************** PP

                                       TIGR02022 316 rlgiGsDshvvvdvleElRllEygqRLrdraRnvlaageeasvaralydaAlaggaqalGlae 378
                                                     rlgiGsDshv+++v+eElR+lEygqRLrd++Rn+l++ ++++v+r+l+daAl ggaqalG+  
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09645 315 RLGIGSDSHVSLSVVEELRWLEYGQRLRDQRRNRLYRADQPMVGRTLFDAALEGGAQALGQPV 377
                                                     *************************************************************** PP

                                       TIGR02022 379 geleaGarADlltldledpslagakgdalldsllfaaekaavrdvvvaGrkvvrdgrHaleee 441
                                                     g+l  G+rAD+l+ld++dp+la+a+gd +l+++lfa+++++vrdv+v+Gr+vvrdgrHa eee
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09645 378 GALDIGKRADWLVLDGNDPYLATANGDGILNRWLFAGGDRQVRDVMVGGRWVVRDGRHAGEEE 440
                                                     *************************************************************** PP

                                       TIGR02022 442 ierafakvlrall 454
                                                     ++raf++vlr+ll
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09645 441 SARAFTQVLRELL 453
                                                     **********996 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (455 nodes)
Target sequences:                          1  (454 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 11.47
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources