Align Imidazolonepropionase (EC 3.5.2.7) (characterized)
to candidate AO356_09595 AO356_09595 imidazolonepropionase
Query= reanno::pseudo5_N2C3_1:AO356_09595 (400 letters) >FitnessBrowser__pseudo5_N2C3_1:AO356_09595 Length = 400 Score = 798 bits (2061), Expect = 0.0 Identities = 400/400 (100%), Positives = 400/400 (100%) Query: 1 MKTLWQNCHAATMAQGVYSIIEDAAIVTQGEHIHWIGPRAELPMGDYPAVNDLKGAWVTP 60 MKTLWQNCHAATMAQGVYSIIEDAAIVTQGEHIHWIGPRAELPMGDYPAVNDLKGAWVTP Sbjct: 1 MKTLWQNCHAATMAQGVYSIIEDAAIVTQGEHIHWIGPRAELPMGDYPAVNDLKGAWVTP 60 Query: 61 GLIDCHTHTVFGGNRSGEFEQRLQGVSYAEIAAAGGGIASTVRATRAASEDELFASAAKR 120 GLIDCHTHTVFGGNRSGEFEQRLQGVSYAEIAAAGGGIASTVRATRAASEDELFASAAKR Sbjct: 61 GLIDCHTHTVFGGNRSGEFEQRLQGVSYAEIAAAGGGIASTVRATRAASEDELFASAAKR 120 Query: 121 LKGLMHDGVTTVEIKSGYGLDLASERKILRVIRRLGAELPVSVRSTCLAAHALPPEYKDR 180 LKGLMHDGVTTVEIKSGYGLDLASERKILRVIRRLGAELPVSVRSTCLAAHALPPEYKDR Sbjct: 121 LKGLMHDGVTTVEIKSGYGLDLASERKILRVIRRLGAELPVSVRSTCLAAHALPPEYKDR 180 Query: 181 ADDYIEHICTEMLPALAAEGLVDAVDAFCEYLAFSPAQVERVFITARQLGLPVKLHAEQL 240 ADDYIEHICTEMLPALAAEGLVDAVDAFCEYLAFSPAQVERVFITARQLGLPVKLHAEQL Sbjct: 181 ADDYIEHICTEMLPALAAEGLVDAVDAFCEYLAFSPAQVERVFITARQLGLPVKLHAEQL 240 Query: 241 SSLHGSSLAARYQALSADHLEFMTEDDAKAMAASGTVAVLLPGAFYFLRETQLPPMDALR 300 SSLHGSSLAARYQALSADHLEFMTEDDAKAMAASGTVAVLLPGAFYFLRETQLPPMDALR Sbjct: 241 SSLHGSSLAARYQALSADHLEFMTEDDAKAMAASGTVAVLLPGAFYFLRETQLPPMDALR 300 Query: 301 KHGVKIAVASDLNPGTSPALSLRLMLNMACTCFRMTPEEALAGATIHAAQALGMAQTHGS 360 KHGVKIAVASDLNPGTSPALSLRLMLNMACTCFRMTPEEALAGATIHAAQALGMAQTHGS Sbjct: 301 KHGVKIAVASDLNPGTSPALSLRLMLNMACTCFRMTPEEALAGATIHAAQALGMAQTHGS 360 Query: 361 LEVGKVADFVAWHIDRPADLAYWLGGDLEKRVVRHGVEID 400 LEVGKVADFVAWHIDRPADLAYWLGGDLEKRVVRHGVEID Sbjct: 361 LEVGKVADFVAWHIDRPADLAYWLGGDLEKRVVRHGVEID 400 Lambda K H 0.320 0.134 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 699 Number of extensions: 15 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 400 Length of database: 400 Length adjustment: 31 Effective length of query: 369 Effective length of database: 369 Effective search space: 136161 Effective search space used: 136161 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 50 (23.9 bits)
Align candidate AO356_09595 AO356_09595 (imidazolonepropionase)
to HMM TIGR01224 (hutI: imidazolonepropionase (EC 3.5.2.7))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01224.hmm # target sequence database: /tmp/gapView.18607.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01224 [M=377] Accession: TIGR01224 Description: hutI: imidazolonepropionase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.8e-150 485.8 0.1 4.4e-150 485.6 0.1 1.0 1 lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09595 AO356_09595 imidazolonepropionas Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09595 AO356_09595 imidazolonepropionase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 485.6 0.1 4.4e-150 4.4e-150 1 375 [. 21 396 .. 21 398 .. 0.99 Alignments for each domain: == domain 1 score: 485.6 bits; conditional E-value: 4.4e-150 TIGR01224 1 iedaailveegkiaaigqkaalpgeeaakiidleGklvvPGlvDpHtHlvfagdRvkefelkl 63 iedaai+ ++++i +ig++a+lp + + dl+G v+PGl+D+HtH vf+g+R+ efe++l lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09595 21 IEDAAIVTQGEHIHWIGPRAELPMGDYPAVNDLKGAWVTPGLIDCHTHTVFGGNRSGEFEQRL 83 5899********************99999********************************** PP TIGR01224 64 qGasYleilaeGgGilstvratraAseeellkeaeerlkkllrsGtttlEvKsGYGLdleaEl 126 qG+sY+ei+a+GgGi stvratraAse+el+++a +rlk l+ G+tt+E+KsGYGLdl +E lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09595 84 QGVSYAEIAAAGGGIASTVRATRAASEDELFASAAKRLKGLMHDGVTTVEIKSGYGLDLASER 146 *************************************************************** PP TIGR01224 127 kmLrvikalkeelpvdvvttflgaHavPkevqededeyvdaileelipkvaeeklaeavDvFc 189 k+Lrvi++l +elpv+v +t+l+aHa+P+e++++ d+y+++i+ e++p+ a e l++avD+Fc lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09595 147 KILRVIRRLGAELPVSVRSTCLAAHALPPEYKDRADDYIEHICTEMLPALAAEGLVDAVDAFC 209 *************************************************************** PP TIGR01224 190 ekgvFsveqsrrilkaaqeaGlavklHaeelkalggaelaaklgavsadHleeasdedikala 252 e +Fs++q++r++ a++ Gl+vklHae+l++l g++laa+ +a+sadHle ++++d+ka+a lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09595 210 EYLAFSPAQVERVFITARQLGLPVKLHAEQLSSLHGSSLAARYQALSADHLEFMTEDDAKAMA 272 *************************************************************** PP TIGR01224 253 eagtvavlLPgtaflLr.eaapparklidekvivalatDlnPgsspllslqlilslavtllkl 314 ++gtvavlLPg++++Lr ++ pp+++l++++v +a+a+DlnPg+sp lsl+l+l++a+t +++ lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09595 273 ASGTVAVLLPGAFYFLReTQLPPMDALRKHGVKIAVASDLNPGTSPALSLRLMLNMACTCFRM 335 *****************9999****************************************** PP TIGR01224 315 taeealaaatvnaAqalglgeekGtleeGkdadlvvlsaesyeeiaYrlgvnvveaviknG 375 t+eeala+at++aAqalg+++++G+le Gk ad+v ++++ ++++aY+lg++ + v+ +G lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09595 336 TPEEALAGATIHAAQALGMAQTHGSLEVGKVADFVAWHIDRPADLAYWLGGDLEKRVVRHG 396 ****************************************************999999887 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (377 nodes) Target sequences: 1 (400 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 10.15 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory