Alignments for a candidate for hutU in Pseudomonas fluorescens FW300-N2C3

GapMind for catabolism of small carbon sources

Alignments for a candidate for hutU in Pseudomonas fluorescens FW300-N2C3

Align Urocanate hydratase (EC 4.2.1.49) (characterized)
to candidate AO356_09630 AO356_09630 urocanate hydratase

Query= reanno::pseudo5_N2C3_1:AO356_09630
         (562 letters)



>FitnessBrowser__pseudo5_N2C3_1:AO356_09630
          Length = 562

 Score = 1148 bits (2970), Expect = 0.0
 Identities = 562/562 (100%), Positives = 562/562 (100%)

Query: 1   VTDATRKPEKYRDVEIRAPRGNTLTAKSWLTEAPLRMLMNNLDPEVAENPKELVVYGGIG 60
           VTDATRKPEKYRDVEIRAPRGNTLTAKSWLTEAPLRMLMNNLDPEVAENPKELVVYGGIG
Sbjct: 1   VTDATRKPEKYRDVEIRAPRGNTLTAKSWLTEAPLRMLMNNLDPEVAENPKELVVYGGIG 60

Query: 61  RAARNWECYDKIVESLTNLNDDETLLVQSGKPVGVFKTHSNAPRVLIANSNLVPHWASWE 120
           RAARNWECYDKIVESLTNLNDDETLLVQSGKPVGVFKTHSNAPRVLIANSNLVPHWASWE
Sbjct: 61  RAARNWECYDKIVESLTNLNDDETLLVQSGKPVGVFKTHSNAPRVLIANSNLVPHWASWE 120

Query: 121 HFNELDAKGLAMYGQMTAGSWIYIGSQGIVQGTYETFVEAGRQHYDSNLKGRWVLTAGLG 180
           HFNELDAKGLAMYGQMTAGSWIYIGSQGIVQGTYETFVEAGRQHYDSNLKGRWVLTAGLG
Sbjct: 121 HFNELDAKGLAMYGQMTAGSWIYIGSQGIVQGTYETFVEAGRQHYDSNLKGRWVLTAGLG 180

Query: 181 GMGGAQPLAATLAGACSLNIECQQVSIDFRLKTRYVDEQAKDLDDALARIEKYTAEGKAI 240
           GMGGAQPLAATLAGACSLNIECQQVSIDFRLKTRYVDEQAKDLDDALARIEKYTAEGKAI
Sbjct: 181 GMGGAQPLAATLAGACSLNIECQQVSIDFRLKTRYVDEQAKDLDDALARIEKYTAEGKAI 240

Query: 241 SIALCGNAAEILPEMVRRGVRPDMVTDQTSAHDPLNGYLPAGWTWDEYRARAKTEPAAVV 300
           SIALCGNAAEILPEMVRRGVRPDMVTDQTSAHDPLNGYLPAGWTWDEYRARAKTEPAAVV
Sbjct: 241 SIALCGNAAEILPEMVRRGVRPDMVTDQTSAHDPLNGYLPAGWTWDEYRARAKTEPAAVV 300

Query: 301 KAAKQSMAVHVKAMLAFQKMGVPTFDYGNNIRQMAQEEGVENAFDFPGFVPAYIRPLFCR 360
           KAAKQSMAVHVKAMLAFQKMGVPTFDYGNNIRQMAQEEGVENAFDFPGFVPAYIRPLFCR
Sbjct: 301 KAAKQSMAVHVKAMLAFQKMGVPTFDYGNNIRQMAQEEGVENAFDFPGFVPAYIRPLFCR 360

Query: 361 GIGPFRWAALSGDPQDIYKTDAKVKELIPDDAHLHNWLDMARERISFQGLPARICWVGLG 420
           GIGPFRWAALSGDPQDIYKTDAKVKELIPDDAHLHNWLDMARERISFQGLPARICWVGLG
Sbjct: 361 GIGPFRWAALSGDPQDIYKTDAKVKELIPDDAHLHNWLDMARERISFQGLPARICWVGLG 420

Query: 421 QRAKLGLAFNEMVRSGELSAPIVIGRDHLDSGSVASPNRETESMQDGSDAVSDWPLLNAL 480
           QRAKLGLAFNEMVRSGELSAPIVIGRDHLDSGSVASPNRETESMQDGSDAVSDWPLLNAL
Sbjct: 421 QRAKLGLAFNEMVRSGELSAPIVIGRDHLDSGSVASPNRETESMQDGSDAVSDWPLLNAL 480

Query: 481 LNTASGATWVSLHHGGGVGMGFSQHSGMVIVCDGTDEAAERIARVLHNDPGTGVMRHADA 540
           LNTASGATWVSLHHGGGVGMGFSQHSGMVIVCDGTDEAAERIARVLHNDPGTGVMRHADA
Sbjct: 481 LNTASGATWVSLHHGGGVGMGFSQHSGMVIVCDGTDEAAERIARVLHNDPGTGVMRHADA 540

Query: 541 GYQIAIDCAKEQGLNLPMITGK 562
           GYQIAIDCAKEQGLNLPMITGK
Sbjct: 541 GYQIAIDCAKEQGLNLPMITGK 562


Lambda     K      H
   0.318    0.134    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1266
Number of extensions: 43
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 562
Length of database: 562
Length adjustment: 36
Effective length of query: 526
Effective length of database: 526
Effective search space:   276676
Effective search space used:   276676
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

Align candidate AO356_09630 AO356_09630 (urocanate hydratase)
to HMM TIGR01228 (hutU: urocanate hydratase (EC 4.2.1.49))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR01228.hmm
# target sequence database:        /tmp/gapView.1086.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01228  [M=545]
Accession:   TIGR01228
Description: hutU: urocanate hydratase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                       Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                       -----------
          0 1027.7   0.3          0 1027.5   0.3    1.0  1  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09630  AO356_09630 urocanate hydratase


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09630  AO356_09630 urocanate hydratase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 ! 1027.5   0.3         0         0       2     545 .]      15     558 ..      14     558 .. 1.00

  Alignments for each domain:
  == domain 1  score: 1027.5 bits;  conditional E-value: 0
                                       TIGR01228   2 eiraprGkeleakgweqeaalrllmnnldpevaedpeelvvyGGkGkaarnweafdkiveelk 64 
                                                     eiraprG++l+ak+w +ea+lr+lmnnldpevae+p+elvvyGG+G+aarnwe++dkive+l+
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09630  15 EIRAPRGNTLTAKSWLTEAPLRMLMNNLDPEVAENPKELVVYGGIGRAARNWECYDKIVESLT 77 
                                                     79************************************************************* PP

                                       TIGR01228  65 rleddetllvqsGkpvgvfkthekaprvliansnlvpkwadwekfeeleakGlimyGqmtaGs 127
                                                     +l+ddetllvqsGkpvgvfkth++aprvliansnlvp+wa+we+f+el+akGl+myGqmtaGs
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09630  78 NLNDDETLLVQSGKPVGVFKTHSNAPRVLIANSNLVPHWASWEHFNELDAKGLAMYGQMTAGS 140
                                                     *************************************************************** PP

                                       TIGR01228 128 wiyiGtqGilqGtyetlaelarkhfggslkgklvltaGlGgmGGaqplavtlneavsiavevd 190
                                                     wiyiG+qGi+qGtyet++e++r+h+  +lkg++vltaGlGgmGGaqpla+tl++a+s+++e++
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09630 141 WIYIGSQGIVQGTYETFVEAGRQHYDSNLKGRWVLTAGLGGMGGAQPLAATLAGACSLNIECQ 203
                                                     *************************************************************** PP

                                       TIGR01228 191 eeridkrletkyldektddldealaraeeakaeGkalsigllGnaaevleellergvvpdvvt 253
                                                     +  id+rl+t+y+de+++dld+alar+e+++aeGka+si+l Gnaae+l+e+++rgv+pd+vt
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09630 204 QVSIDFRLKTRYVDEQAKDLDDALARIEKYTAEGKAISIALCGNAAEILPEMVRRGVRPDMVT 266
                                                     *************************************************************** PP

                                       TIGR01228 254 dqtsahdellGyipegytvedadklrdeepeeyvkaakaslakhvrallalqkkGavtfdyGn 316
                                                     dqtsahd+l+Gy+p+g+t+++++ ++++ep+++vkaak+s+a+hv+a+la+qk G+ tfdyGn
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09630 267 DQTSAHDPLNGYLPAGWTWDEYRARAKTEPAAVVKAAKQSMAVHVKAMLAFQKMGVPTFDYGN 329
                                                     *************************************************************** PP

                                       TIGR01228 317 nirqvakeeGvedafdfpGfvpayirdlfceGkGpfrwvalsGdpadiyrtdkavkelfpede 379
                                                     nirq+a+eeGve+afdfpGfvpayir+lfc+G Gpfrw+alsGdp+diy+td++vkel+p+d+
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09630 330 NIRQMAQEEGVENAFDFPGFVPAYIRPLFCRGIGPFRWAALSGDPQDIYKTDAKVKELIPDDA 392
                                                     *************************************************************** PP

                                       TIGR01228 380 elhrwidlakekvafqGlparicwlgygereklalainelvrsGelkapvvigrdhldaGsva 442
                                                     +lh+w+d+a+e+++fqGlparicw+g+g+r+kl+la+ne+vrsGel+ap+vigrdhld+Gsva
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09630 393 HLHNWLDMARERISFQGLPARICWVGLGQRAKLGLAFNEMVRSGELSAPIVIGRDHLDSGSVA 455
                                                     *************************************************************** PP

                                       TIGR01228 443 spnreteamkdGsdavadwpllnallntaaGaswvslhhGGGvglGfslhaglvivadGtdea 505
                                                     spnrete+m+dGsdav+dwpllnallnta+Ga+wvslhhGGGvg+Gfs+h+g+viv+dGtdea
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09630 456 SPNRETESMQDGSDAVSDWPLLNALLNTASGATWVSLHHGGGVGMGFSQHSGMVIVCDGTDEA 518
                                                     *************************************************************** PP

                                       TIGR01228 506 aerlkrvltadpGlGvirhadaGyesaldvakeqgldlpm 545
                                                     aer++rvl +dpG+Gv+rhadaGy+ a+d+akeqgl+lpm
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_09630 519 AERIARVLHNDPGTGVMRHADAGYQIAIDCAKEQGLNLPM 558
                                                     ***************************************8 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (545 nodes)
Target sequences:                          1  (562 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02
# Mc/sec: 12.51
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources