Alignments for a candidate for ech in Pseudomonas fluorescens FW300-N2C3

GapMind for catabolism of small carbon sources

Alignments for a candidate for ech in Pseudomonas fluorescens FW300-N2C3

Align Peroxisomal bifunctional enzyme; PBE; PBFE; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 (characterized)
to candidate AO356_30295 AO356_30295 fatty-acid oxidation protein subunit alpha

Query= SwissProt::Q08426
         (723 letters)



>FitnessBrowser__pseudo5_N2C3_1:AO356_30295
          Length = 703

 Score =  448 bits (1153), Expect = e-130
 Identities = 274/709 (38%), Positives = 403/709 (56%), Gaps = 36/709 (5%)

Query: 7   LHNALALIRLRNPPVNAISTTLLRDIKEGLQKAVIDHTIKAIVICGAEGKFSAGADIRGF 66
           +   +A+I + NPPVNA++  +   I + +  A  D  + AIV+ G    F AGADIR F
Sbjct: 11  VEGVVAVITVNNPPVNALTPEMRGSIIDAVTHANADPAVAAIVLMGGGQNFIAGADIRQF 70

Query: 67  SAPRTFGLTLGHVVDEIQRNEKPVVAAIQGMAFGGGLELALGCHYRIAHAEAQVGLPEVT 126
              RT  +T       +  + KP VAAI G A GGGLE AL CHYRIA   A++GLPEV 
Sbjct: 71  GKART--ITTSMSAAALDASAKPTVAAIHGYALGGGLEHALACHYRIALPTAKLGLPEVA 128

Query: 127 LGLLPGARGTQLLPRLTGVPAALDLITSGRRILADEALKLGILDKVV----NSDPVEEAI 182
           LGL+PG  GTQ LPRL G   A DL+ S R I A EAL++G++ ++V    N +  + A+
Sbjct: 129 LGLIPGGGGTQRLPRLVGAAVATDLLVSSRHIHAPEALQIGLISQIVPGTQNQELRDAAV 188

Query: 183 RFAQRVSD-QPLESRRLCNKPIQSLPNMDSIFSEALLKMRRQHPGCLAQEACVRAVQAAV 241
           R+A+ V+  QP   R L  +P+ +   MD +   A  +  ++ P   A +  +  ++AA 
Sbjct: 189 RYAKAVAHAQPQRVRDLALEPMDAT-TMD-LLQAARREAAKKSPHNKAPQYAIDCIEAAF 246

Query: 242 QYPYEVGIKKEEELFLYLLQSGQARALQYAFFAERKANKW-STPSGASWKTASARPVSSV 300
             P++  ++ E  LF  L  S  A+AL+YAFFAER+A K    P G  +K A    V+S 
Sbjct: 247 NLPFDEALEVESRLFSELEGSDVAKALRYAFFAEREAAKVPDAPPG--FKPAG---VTSA 301

Query: 301 GVVGLGTMGRGIVISFARARIPVIAVDSDKNQLATANKMITSVLEKEASK----MQQSGH 356
            V+G GTMG GI I FA A I V  +++    L    + I  +   +  +    +Q+   
Sbjct: 302 AVIGAGTMGAGIAICFADAGIVVKLLEASAEALDRGLQRIRDIYAVKVKRGRMTVQEMDA 361

Query: 357 PWSGPKPRLTSSVKELGGVDLVIEAVFEEMSLKKQVFAELSAVCKPEAFLCTNTSALDVD 416
                +P   S  +E+G  D+V+EAVFE + LKK VF +L AV KP A L TN+SA+D++
Sbjct: 362 CLQFVQP--VSHYEEIGDCDVVVEAVFERIDLKKDVFTKLDAVMKPGALLLTNSSAIDIN 419

Query: 417 EIASSTDRPHLVIGTHFFSPAHVMKLLEVIPSQYSSPTTIATVMNLSKKIKKIGVVVGNC 476
            +A+ T RP  V G HFF+PA+ MKL E++    +S  T+   M + + +KK+  V G C
Sbjct: 420 VMANMTGRPQSVAGAHFFAPANAMKLCEIVKGDQTSIETVVRAMKMGRDLKKVCAVSGTC 479

Query: 477 FGFVGNRMLNPYYNQAYFLLEEGSKPEEVDQVLEEFGFKMGPFRVSDLAGLDVGWKSRKG 536
            GF  NR   P   +   LLEEG++PE++D+V+ EFG+ MGPF VSDL+GLDV + +RK 
Sbjct: 480 DGFAANRSRAPLVTEMMLLLEEGARPEQIDRVMVEFGYPMGPFAVSDLSGLDVSYDTRKR 539

Query: 537 QGLTGPTLLPGTPARKRGNRRYCPIPDVLCELGRFGQKTGKGWYQYDKPLGRIHKPDPWL 596
           +    P              R   +PD + ELGR GQK G GWY+Y++   R  + D  +
Sbjct: 540 RAAADP------------QYRKLHVPDRMVELGRKGQKNGAGWYRYEEG-DRTPRHDEEV 586

Query: 597 SKFLSRYRKTHHIEPRTISQDEILERCLYSLINEAFRILGEGIAASPEHIDVVYLHGYGW 656
            + ++          RT +  EIL+R L++ INEA +IL EG A     IDV++L+G+G+
Sbjct: 587 MRIIATVAHELGTPQRTFTDSEILKRLLFASINEACKILDEGKAYRASDIDVMWLYGFGF 646

Query: 657 PRHKGGPMFYASTVGLPTVLEKLQKYYRQNPDIPQLEPSDYLKKLASQG 705
           PRH+GG +++A T+G   +  ++Q+++ Q     +  PS  L K A++G
Sbjct: 647 PRHRGGLLYWADTIGATEIHLQVQQWHEQYG--RRWAPSPLLSKAANEG 693


Lambda     K      H
   0.319    0.136    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1056
Number of extensions: 57
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 723
Length of database: 703
Length adjustment: 39
Effective length of query: 684
Effective length of database: 664
Effective search space:   454176
Effective search space used:   454176
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources