Aligments for a candidate for pccA1 in Pseudomonas fluorescens FW300-N2C3

GapMind for catabolism of small carbon sources

Aligments for a candidate for pccA1 in Pseudomonas fluorescens FW300-N2C3

Align acyl CoA carboxylase biotin carboxylase subunit (EC 2.1.3.15; EC 6.4.1.3; EC 6.3.4.14) (characterized)
to candidate AO356_08195 AO356_08195 acetyl-CoA carboxylase biotin carboxylase subunit

Query= metacyc::MONOMER-13597
         (509 letters)



>FitnessBrowser__pseudo5_N2C3_1:AO356_08195
          Length = 451

 Score =  379 bits (973), Expect = e-109
 Identities = 202/445 (45%), Positives = 288/445 (64%), Gaps = 6/445 (1%)

Query: 6   RVLVANRGEIATRVLKAIKEMGMTAIAVYSEADKYAVHTKYADEAYYIGKAPALDSYLNI 65
           +VL+ANRGEIA R+L+A KEMG+  +AVYS+ADK  +H   ADE+  IG A A  SYL+I
Sbjct: 7   KVLIANRGEIALRILRACKEMGIKTVAVYSKADKELMHLGLADESVCIGPASAAHSYLHI 66

Query: 66  EHIIDAAEKAHVDAIHPGYGFLSENAEFAEAVEKAGITFIGPSSEVMRKIKDKLDGKRLA 125
             II AAE     AIHPGYGFL+ENA+FAE VE +G  FIGP ++ +R + DK+  K   
Sbjct: 67  PAIIAAAEVTGATAIHPGYGFLAENADFAEQVENSGFAFIGPKADTIRLMGDKVSAKHAM 126

Query: 126 NMAGVPTAPGSDGPVTSIDE-ALKLAEKIGYPIMVKAASGGGGVGITRVDNQDQLMDVWE 184
             AGVPT PGSDGP+   +E AL++  ++GYP+++KAA GGGG G+  V  ++ L+   +
Sbjct: 127 IAAGVPTVPGSDGPLPEDEETALRIGREVGYPVIIKAAGGGGGRGMRVVHKEEDLISSAK 186

Query: 185 RNKRLAYQAFGKADLFIEKYAVNPRHIEFQLIGDKYGNYVVAWERECTIQRRNQKLIEEA 244
             +  A  AFG   +++EK+  NPRH+E Q++ D  G  +   +R+C++QRR+QK++EEA
Sbjct: 187 LTRSEAGAAFGNPMVYLEKFLTNPRHVEVQVLSDGQGQAIHLGDRDCSLQRRHQKVLEEA 246

Query: 245 PSPALKMEERESMFEPIIKFGKLINYFTLGTFETAFSDVSRDFYFLELNKRLQVEHPTTE 304
           P+P +  + R+ +    +K    I Y   GTFE  +   +  FYF+E+N R+QVEHP +E
Sbjct: 247 PAPGIDEQARQEVLARCVKACIDIGYRGAGTFEFLYE--NGRFYFIEMNTRVQVEHPVSE 304

Query: 305 LIFRIDLVKLQIKLAAGEHLPFSQEDLNKRVRGTAIEYRINAEDALNNFTGSSGFVTYYR 364
           ++  ID+VK  + +AAG  L F+Q+D+   +RG A+E RINAED    F  S G V ++ 
Sbjct: 305 MVTGIDIVKEMLSIAAGNKLSFTQDDV--VIRGHALECRINAEDP-KTFMPSPGTVKHFH 361

Query: 365 EPTGPGVRVDSGIESGSYVPPYYDSLVSKLIVYGESREYAIQAGIRALADYKIGGIKTTI 424
            P G GVRVDS + SG  VPP YDSL+ KLI YG +RE A+     AL +  + GIKT I
Sbjct: 362 APGGNGVRVDSHLYSGYAVPPNYDSLIGKLITYGATREEAMARMRNALDEIVVDGIKTNI 421

Query: 425 ELYKWIMQDPDFQEGKFSTSYISQK 449
            L++ +++D  F +G  +  Y+  K
Sbjct: 422 PLHRDLVRDEGFCKGGVNIHYLEHK 446


Lambda     K      H
   0.317    0.135    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 530
Number of extensions: 19
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 509
Length of database: 451
Length adjustment: 34
Effective length of query: 475
Effective length of database: 417
Effective search space:   198075
Effective search space used:   198075
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources