GapMind for catabolism of small carbon sources

 

Alignments for a candidate for galdh in Pseudomonas fluorescens FW300-N2C3

Align L-arabinose 1-dehydrogenase / D-galactose 1-dehydrogenase (EC 1.1.1.46; EC 1.1.1.48) (characterized)
to candidate AO356_09100 AO356_09100 3-oxoacyl-ACP reductase

Query= reanno::pseudo6_N2E2:Pf6N2E2_5967
         (272 letters)



>FitnessBrowser__pseudo5_N2C3_1:AO356_09100
          Length = 249

 Score =  132 bits (333), Expect = 6e-36
 Identities = 90/234 (38%), Positives = 126/234 (53%), Gaps = 6/234 (2%)

Query: 17  ERLKNKVVLLTGAAQGIGEAIVAAFASQQARLVISDIQAEKVETVAAHWRERGADVHALK 76
           ++L N+  ++TGA  GIG AI  A+A+Q ARLV++D  AE++   A   R    +V+   
Sbjct: 2   QQLFNRRAVITGAGSGIGAAIARAYAAQGARLVLTDRDAERLSATANDCRALSVEVYECV 61

Query: 77  ADVSNQQDLHAMARHAVERHGRIDVLVNCAGVNVFRDPLEMTEEDWRRCFAIDLDGAWYG 136
           ADV + +   A     VE+ G ID+LVN AG+       E++ E W     +DL   +  
Sbjct: 62  ADVGSVEGAQAGVDACVEQFGGIDILVNNAGMLTQARCTELSIEMWNDMLRVDLTSVFIA 121

Query: 137 CKAVLPQMIEQGVGSIINIASTHSSHIIPGCFPYPVAKHGLLGLTRALGIEYAPKGVRVN 196
            +  LP M+ Q  G IIN+AS            Y  AK G++G T++L +E A   V VN
Sbjct: 122 SQRALPHMLAQRWGRIINVASQLGIKGGAELSHYAAAKAGVIGFTKSLALEVAADNVLVN 181

Query: 197 AIAPGYIETQLNVDYWNGFADPYAERQRALDLHPPRRIGQPIEVAMTAVFLASD 250
           AIAPG IET L      G ++ + + + A +L P RR G   EVA TAV LASD
Sbjct: 182 AIAPGPIETPLVA----GISESWKQAKSA-EL-PLRRFGLATEVAPTAVLLASD 229


Lambda     K      H
   0.321    0.137    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 161
Number of extensions: 12
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 272
Length of database: 249
Length adjustment: 24
Effective length of query: 248
Effective length of database: 225
Effective search space:    55800
Effective search space used:    55800
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory