Align L-2-aminoadipate reductase; Alpha-aminoadipate reductase; Alpha-AR; L-aminoadipate-semialdehyde dehydrogenase; EC 1.2.1.31; EC 1.2.1.95 (characterized)
to candidate AO356_28210 AO356_28210 hypothetical protein
Query= SwissProt::P40976
(1419 letters)
>FitnessBrowser__pseudo5_N2C3_1:AO356_28210
Length = 10307
Score = 252 bits (644), Expect = 1e-69
Identities = 191/660 (28%), Positives = 316/660 (47%), Gaps = 102/660 (15%)
Query: 315 YRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPPA 374
YR+++ SN LAH+L K G+E V + RG+++VV ++ +LKAG + +DPAYP
Sbjct: 5968 YRELNGLSNRLAHYLRKQGVEPDSRVAICVERGIEMVVGLLAILKAGGGYVPLDPAYPLD 6027
Query: 375 RQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKTYVPALKLAKDGSLTGGSVSKG 434
R L + P A++V + ++L +T +P + L K G +
Sbjct: 6028 RIAYMLEDSAPAAILV------------QGTTRNLLGETAIPVIDLDK-----GIWQDEA 6070
Query: 435 ADDILQHVLHLKSEQTGVVVGPDSTPTLSFTSGSEGIPKGVKGRHFSLAYYFDWMAQEFN 494
A + V L S V+ +TSGS G+PKGV H + + W + F+
Sbjct: 6071 APN--PQVPGLSSSHLAYVI---------YTSGSTGLPKGVMIEHRNTVNFLTWAHRSFD 6119
Query: 495 LSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPTAEDIGTPGQLAQWANKYKVTVTHL 554
+ + + D + F PL G S+ V T L ++ +T+ +
Sbjct: 6120 AQILSKTLFSTSLNFDLAVYECFAPLTSGGSIEVVT-------NVLELQQGEHDITLINT 6172
Query: 555 TPAMGQLLAAQAD--EPIPSLHHAFFVGDILTKRDCLRLQVLANNVNVVNMYGTTETQRS 612
P+ + L E + +++ A G+ L + L + N+YG +ET
Sbjct: 6173 VPSALKALLESGGLGEGVDTVNVA---GEALKRSLVETLFEQTQVKRLCNLYGPSETTTY 6229
Query: 613 VSYFVVPARSQDQTFLESQKDVIPA--GRGMKNVQLLVINRFDTNKICGIGEVGEIYLRA 670
S+ + +++D A G+ + N Q +++ + + +G GEIY+
Sbjct: 6230 SSWVSM-----------AREDGFAAHIGKPVANTQFYLLD--EHKQPVPLGVPGEIYIGG 6276
Query: 671 GGLAEGYLGNDELTSKKFLKSWFADPSKFVDRTPENAPWKPYWFGIRDRMYRSGDLGRYL 730
G+A GYL D+LT+++FLK+ F+ T NA RMYR+GDLGRYL
Sbjct: 6277 AGVARGYLNRDDLTAERFLKNPFS--------TAPNA-----------RMYRTGDLGRYL 6317
Query: 731 PTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRENITLVRRDKDEEPTLVAYIVP 790
P GN+E GR DDQ+KIRGFRIELGEI L++H ++E + + R D + LVAY
Sbjct: 6318 PDGNIEYLGRNDDQVKIRGFRIELGEIEAKLAQHDALKEAVVVAREDVPGDKRLVAY--- 6374
Query: 791 QGLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKTKLPSYAIPSVIVPLHKMPLNPN 850
F + E + + L R +L+T+LP+Y +P+V V L +PL PN
Sbjct: 6375 -------FTQHSPDETVEIEAL----------RTHLQTQLPAYMVPAVYVRLDALPLTPN 6417
Query: 851 GKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERDIRDIWLRIIPHATDVNKKASFF 910
GK+D+ ALP PD + + + V+ TL +D+ D+ V + FF
Sbjct: 6418 GKLDRKALPAPDLDAVISRGYEAPQGEVETTLAQIWQDLLDL--------QQVGRHDQFF 6469
Query: 911 DIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGLAKEIERMKSGEMISVMDIGKEE 970
++GGHS+LA +LI ++R + V + L +F++P + GLA+ + + ++ + +++
Sbjct: 6470 ELGGHSLLAIQLISQVRLRLGVELGLTELFAQPQLMGLAQAVALAGRSTLPEIVRVSRKD 6529
Score = 252 bits (644), Expect = 1e-69
Identities = 248/963 (25%), Positives = 403/963 (41%), Gaps = 143/963 (14%)
Query: 20 ERLKSQTISH------LPTDYSRPVPS----RLVEAVFERTLPEDAKTALIKVYVAAQAK 69
ER QT++ LPTD RP V V + TL + K +Q
Sbjct: 7791 ERFWRQTLADAPALLTLPTDRPRPAQQDHAGAAVGIVLDETLTQGLKAL-------SQRH 7843
Query: 70 GILVTPFNILLTIFIILVSRMTGDEDISIGTSSENAIPFVLRTFIQPSDSFLDLLAKVCD 129
G T F ++ + ++SR++G D+ IGT N + + I F++ LA D
Sbjct: 7844 G--ATLFMTVMAAWATVLSRLSGQHDVVIGTPVANRMQAEVEGLI---GLFVNTLALRVD 7898
Query: 130 LEKEGSSDAVDFSDLINFLNAKLSKKDD---------PRKTLVHLRFYNAPDAPSENFLS 180
+ E + A+ + L+A+ + P ++L H + A + N
Sbjct: 7899 VADELTVQALLQQVKVRTLDAQAHQDLPFEQVVEVVRPLRSLSHSPVFQAMLSWQNN--E 7956
Query: 181 TTGLDVDLTVLVSVKKPSDQLTSLRSQF-TFPDLQL-------KLIYNQLLFSESRVNIV 232
TGL++ L + +TS ++F D+ L L Y LF + +
Sbjct: 7957 ATGLELGDMSLQGLG-----VTSRTAKFDVLLDMALIDGRLFGALEYATALFDSATMERY 8011
Query: 233 ADQLLKLVVSASKDVTGPIGALDLMTPTQMNVLPDPTVDLDWSGYRGAI--QDIFASNAA 290
L ++ + D + + L+ + L + T++ Y + +F +
Sbjct: 8012 LGYLESVLRAMVADEQTLVAQIPLLGDNERQNLLE-TLNATAVDYPQGLTLHGLFEARVV 8070
Query: 291 KFPDRECIVVTPSVTIDAPVTSYTYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDL 350
PD +V + + +Y Q++E +N +AH L++ GI D V + A R +D+
Sbjct: 8071 ASPD--------AVAVVSGEAQLSYAQLNERANQIAHRLLELGIRPDDRVAICADRSLDM 8122
Query: 351 VVAVMGVLKAGATFSVIDPAYPPARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLE 410
V ++G+LKAGA + +DP YP R L + P +VVL + + SL
Sbjct: 8123 VAGLLGILKAGAAYVPLDPDYPHERLAYMLENSAP--MVVLTQRAL---------QASLP 8171
Query: 411 LKTYVPALKLAKDGSLTGGSVSKGADDILQHVLHLKSEQTGVVVGPDSTPTLSFTSGSEG 470
L G T + + + + H HL + +TSGS G
Sbjct: 8172 ATQARTLLLDEAQGFSTQPTDNPQVEGL--HAHHLAY--------------VIYTSGSTG 8215
Query: 471 IPKGVKGRHFSLAYYFDWMAQEFNLSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPT 530
+PKGV H + WM E+ L+ +D + + D + F PLF GA L++
Sbjct: 8216 LPKGVMNEHGGVVNRLLWMQDEYGLTATDAVLQKTPFSFDVSVWEFFWPLFSGARLVMAR 8275
Query: 531 AEDIGTPGQLAQWANKYKVTVTHLTPAMGQLLAAQAD-EPIPSLHHAFFVGDILTKRDCL 589
P L + +T H P+M + A + L G+ L
Sbjct: 8276 PGGHRDPAYLREVIQAQGITTLHFVPSMLDVFLAHGEASQCAGLVRVMCSGEALPGHLVR 8335
Query: 590 RLQVLANNVNVVNMYGTTETQRSVSYFVVPARSQDQTFLESQKDVIPAGRGMKNVQLLVI 649
R + + + N+YG TE V+ + D P G+ + N ++ ++
Sbjct: 8336 RFKAQLPHTGLYNLYGPTEAAVDVTAWDCAGTDTP--------DSTPIGKPIANTRIYLL 8387
Query: 650 NRFDTNKICGIGEVGEIYLRAGGLAEGYLGNDELTSKKFLKSWFADPSKFVDRTPENAPW 709
+ G+ GEIY+ +A GYL ELT ++FL+ F+ + NA
Sbjct: 8388 DGHQQPVPMGVA--GEIYIGGVQVARGYLNRPELTVERFLRDLFSRAA--------NA-- 8435
Query: 710 KPYWFGIRDRMYRSGDLGRYLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRE 769
RMYR+GDLGRYLP GN+E GR DDQ+KIRGFRIELGEI L++H ++E
Sbjct: 8436 ---------RMYRTGDLGRYLPDGNIEYLGRNDDQVKIRGFRIELGEIEAKLAQHEAIKE 8486
Query: 770 NITLVRRDKDEEPTLVAYIVPQGLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKTK 829
+ L D LVAY G ++ I ++R L+ +
Sbjct: 8487 AVVLALEDVPGAKRLVAYFTVHG--------------------DEHSVEIENLRTQLQAQ 8526
Query: 830 LPSYAIPSVIVPLHKMPLNPNGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERDI 889
LP Y +P+ V L +PL PNGK+D+ ALP PD + + + V+ TL
Sbjct: 8527 LPEYMVPAAYVRLQALPLTPNGKLDRKALPAPDLASVITREYEAPQGAVETTLA------ 8580
Query: 890 RDIWLRIIPHATDVNKKASFFDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGLA 949
+W ++ V + FF++GGHS+LA LI +R+ ++ + ++F +PT+ LA
Sbjct: 8581 -QLWAELL-KVEKVGRHDHFFELGGHSLLAVTLIERMRQA-GLSADVRVLFGQPTLAALA 8637
Query: 950 KEI 952
+
Sbjct: 8638 AAV 8640
Score = 246 bits (629), Expect = 7e-68
Identities = 216/735 (29%), Positives = 331/735 (45%), Gaps = 102/735 (13%)
Query: 225 SESRVNIVADQLLKLVVSASKDVTGPIGALDLMTPTQMNVLPDPTVDLDWSGYRGAIQDI 284
+E N V + LV + + P+ L ++ P + L + W+G A
Sbjct: 4798 AERVCNYVQCVVENLVQALEQSPLAPLSGLKILPPAERRQLLE-----SWNGTGVA---- 4848
Query: 285 FASNAAKFPDRECIVVTP--SVTIDAPVTSYTYRQIDESSNILAHHLVKNGIERGDVVMV 342
FA +A E ++ + + TY +++ +N +AH L+ GI D V +
Sbjct: 4849 FAQDALIHAQFEVWAAAQPEAMAVMFEERTLTYSELNARANQVAHRLLAQGIRPDDRVAI 4908
Query: 343 YAYRGVDLVVAVMGVLKAGATFSVIDPAYPPARQIIYLSVAKPRALVVLEDAGVLSPTVV 402
RG+D++V ++G+LKAGA + +DPAYP R L + P AL+
Sbjct: 4909 CVERGLDMIVGLLGILKAGAGYVPVDPAYPTERIAYMLRDSMPVALLA------------ 4956
Query: 403 EYVEKSLELKTYVPALKLAKDGSLTGGSVSKGADDILQHVLHLKSEQTGVVVGPDSTPTL 462
L +P L+L DGS SV+ V L S V+
Sbjct: 4957 HAATHRLLAGVSMPVLQL-DDGSWQDESVAN------PQVPGLDSSHLAYVI-------- 5001
Query: 463 SFTSGSEGIPKGVKGRHFSLAYYFDWMAQEFNLSESDRFTMLSGIAHDPIQRDIFTPLFL 522
+TSGS G+PKGV H ++A F F+ + D + + A D +I+ L
Sbjct: 5002 -YTSGSTGLPKGVMVEHRNVARLFSATQPWFDFGQQDVWALFHSFAFDFSVWEIWGALTH 5060
Query: 523 GASLIVPTAEDIGTPGQLAQWANKYKVTVTHLTP-AMGQLLAAQADEPIP-SLHHAFFVG 580
G L+V +P + VTV + TP A QL+ AQ + + SL F G
Sbjct: 5061 GGRLLVVPQLVSRSPQDCYALLCETGVTVLNQTPSAFRQLIVAQGESDLRHSLRQVIFGG 5120
Query: 581 DILTKRDCLRLQVLANNVN--VVNMYGTTETQRSVSYFVVPARSQDQTFLESQKDVIPAG 638
+ L N +VNMYG TET V+Y + A T V P G
Sbjct: 5121 EALETGMLKPWYARVGNAGTQLVNMYGITETTVHVTYRALEAADAQST------GVSPIG 5174
Query: 639 RGMKNVQLLVINRFDTNKICGIGEVGEIYLRAGGLAEGYLGNDELTSKKFLKSWFADPSK 698
+ + ++QL V++ + +G VGE+Y+ G++ GYL DELT+++FL + F+
Sbjct: 5175 KRIPDLQLYVLDA--QREPAPVGVVGEMYVGGAGVSRGYLNRDELTAERFLDNPFS---- 5228
Query: 699 FVDRTPE-NAPWKPYWFGIRDRMYRSGDLGRYLPTGNVECSGRADDQIKIRGFRIELGEI 757
PE NA RMYR+GDLGR+L G++E GR DDQ+KIRGFRIELGEI
Sbjct: 5229 -----PEPNA-----------RMYRTGDLGRWLADGSIEYLGRNDDQVKIRGFRIELGEI 5272
Query: 758 NTHLSRHPNVRENITLVRRDKDEEPTLVAYIVPQGLNKDDFDSATESEDIVVNGLKKYRK 817
L+ V E + + R D+ + LVAY++ ATE +
Sbjct: 5273 EATLAACEGVSEAVVIAREDEPGDKRLVAYVI-----------ATEGA----------QP 5311
Query: 818 LIHDIREYLKTKLPSYAIPSVIVPLHKMPLNPNGKIDKPALPFPDTSQLAAASRSHSKHG 877
D+R L L + +PS V L PL NGK+D+ ALP PD S + + +
Sbjct: 5312 SAVDLRTQLLRSLAEHMVPSAFVILDAFPLTTNGKLDRKALPAPDQSAVISREYEAPQGE 5371
Query: 878 VDETLTATERDIRDIWLRIIPHATDVNKKASFFDIGGHSILATRLIFELRKKFAVNVPLG 937
V+ T+ +D+ + V + +FF++GGHS+LA +LI E ++ + +
Sbjct: 5372 VETTIARIWQDLLKL--------GRVGRHDNFFELGGHSLLAVKLI-ERMRQVDLAADVR 5422
Query: 938 LVFSEPTIEGLAKEI 952
++F +PT+ LA +
Sbjct: 5423 VLFGQPTLAALAAAV 5437
Score = 241 bits (616), Expect = 2e-66
Identities = 213/724 (29%), Positives = 325/724 (44%), Gaps = 99/724 (13%)
Query: 236 LLKLVVSASKDVTGPIGALDLMTPTQMNVLPDP--TVDLDWSGYRGAIQDIFASNAAKFP 293
L LV++ + P+ AL ++ + L + T D+ ++ I F AA P
Sbjct: 3719 LENLVMALEQAPQTPLHALAILPSAERRQLLETWNTTDVTYAD-DALIHRQFEGWAASQP 3777
Query: 294 DRECIVVTPSVTIDAPVTSYTYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVA 353
D +V + TY +++ +N LAH LV G+ D V + RG+D+++
Sbjct: 3778 DTVAVVHEEQ--------ALTYGELNARANQLAHSLVALGVRPDDRVAICVERGLDMIIG 3829
Query: 354 VMGVLKAGATFSVIDPAYPPARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKT 413
++G+LKAGA + +DP YPP R L + P A+V P +
Sbjct: 3830 LLGILKAGAGYVPLDPTYPPERIAYMLHDSSPVAVVTQSQWRDSLPVLP----------- 3878
Query: 414 YVPALKLAKDGSLTGGSVSKGADDILQHVLHLKSEQTGVVVGPDSTPTLSFTSGSEGIPK 473
VP L L + + D+ L + V+ +TSGS G+PK
Sbjct: 3879 -VPTLVLDPTDAGEMNQQPRHNPDVAD----LNASHLAYVI---------YTSGSTGLPK 3924
Query: 474 GVKGRHFSLAYYFDWMAQEFNLSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPTAED 533
GV H ++A F F D + + A D +I+ L G L+V
Sbjct: 3925 GVMVEHRNVARLFSATQPWFEFGPQDVWALFHSFAFDFSVWEIWGALTHGGRLLVVPQLV 3984
Query: 534 IGTPGQLAQWANKYKVTVTHLTP-AMGQLLAAQADEPIP-SLHHAFFVGDILTKRDCLRL 591
+P + VT+ + TP A QL+AAQ + + SL F G+ L
Sbjct: 3985 SRSPQYCYALLCEAGVTILNQTPSAFRQLIAAQGESDLSHSLRQVIFGGEALETGILKPW 4044
Query: 592 QVLANNVN--VVNMYGTTETQRSVSYFVVPARSQDQTFLESQKDVIPAGRGMKNVQLLVI 649
N +VNMYG TET V+Y + A V P G+ + ++QL V+
Sbjct: 4045 YARQANARTQLVNMYGITETTVHVTYRPLDAADAQLA------GVSPIGKRIPDLQLYVL 4098
Query: 650 NRFDTNKICGIGEVGEIYLRAGGLAEGYLGNDELTSKKFLKSWFADPSKFVDRTPE-NAP 708
+ + +G GE+Y+ GG++ GYL DELT+++FL + F+ PE NA
Sbjct: 4099 DT--QREPVPVGVTGEMYVGGGGVSRGYLNRDELTAERFLDNPFS---------PEPNA- 4146
Query: 709 WKPYWFGIRDRMYRSGDLGRYLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVR 768
RMYR+GDLGR+L G++E GR DDQ+KIRGFRIELGEI L+ VR
Sbjct: 4147 ----------RMYRTGDLGRWLADGSIEYLGRNDDQVKIRGFRIELGEIEAKLAACNGVR 4196
Query: 769 ENITLVRRDKDEEPTLVAYIVPQGLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKT 828
E + + R+D + LVAY++ + + + SA E +R+ L
Sbjct: 4197 EAVVVARQDTPGDQRLVAYVIAE---EGEPPSAAE------------------LRDELLG 4235
Query: 829 KLPSYAIPSVIVPLHKMPLNPNGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERD 888
L Y +PS V L PL NGK+D+ ALP PD S + + V E
Sbjct: 4236 SLAEYMVPSAFVMLTVFPLTTNGKLDRKALPVPDQSSVVTREYEAPQGEV-------EIA 4288
Query: 889 IRDIWLRIIPHATDVNKKASFFDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGL 948
I IW ++ H V + FF++GGHS+LA +L+ E ++ + + ++F +PT+ L
Sbjct: 4289 IARIWQDLL-HVERVGRHDHFFEMGGHSLLAVKLV-ERMRQIDLAADVRVLFGQPTLAAL 4346
Query: 949 AKEI 952
A +
Sbjct: 4347 AAAV 4350
Score = 239 bits (610), Expect = 1e-65
Identities = 193/662 (29%), Positives = 307/662 (46%), Gaps = 117/662 (17%)
Query: 314 TYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPP 373
+Y +++E +N LAH+L K G++ V + RG+D+VV ++ +LKAG + +DPAYP
Sbjct: 1646 SYGELNERANRLAHYLRKQGVKPDSRVAICVERGIDMVVGLLAILKAGGGYVPLDPAYPL 1705
Query: 374 ARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKTYVPALKLAKDGSLTGGSVSK 433
R I Y+ A V+ +DA V L D S+ ++
Sbjct: 1706 DR-IAYMLEDSAPAAVLAQDATV----------------------GLLSDASMAVINLDS 1742
Query: 434 G--ADDILQH--VLHLKSEQTGVVVGPDSTPTLSFTSGSEGIPKGVKGRHFSLAYYFDWM 489
G D+ +Q+ V L S V+ +TSGS G+PKGV H + + W
Sbjct: 1743 GDWQDESVQNPEVTELTSSHLAYVI---------YTSGSTGLPKGVMIEHRNTVNFLTWA 1793
Query: 490 AQEFNLSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPTAEDIGTPGQLAQWANKYKV 549
+ F+ + + + D + F PL G S+ V T L ++ +
Sbjct: 1794 HRSFDSQTLAKTLFSTSLNFDLAVYECFAPLTSGGSIEVVT-------NVLELQQGEHDI 1846
Query: 550 TVTHLTPAMGQLLAAQAD--EPIPSLHHAFFVGDILTKRDCLRLQVLANNVNVVNMYGTT 607
T+ + P+ + L E + +++ A G+ L + L + N+YG +
Sbjct: 1847 TLINTVPSALKALLESGGLGEGVDTVNVA---GEALKRSLVETLFEQTQVKRLCNLYGPS 1903
Query: 608 ETQRSVSYFVVPARSQDQTFLESQKDVIPA--GRGMKNVQLLVINRFDTNKICGIGEVGE 665
ET S+ + +++D A G+ + N Q +++ + + +G GE
Sbjct: 1904 ETTTYSSWVSM-----------AREDGFAAHIGKPVANTQFYLLD--EHKQPVPLGVPGE 1950
Query: 666 IYLRAGGLAEGYLGNDELTSKKFLKSWFADPSKFVDRTPENAPWKPYWFGIRDRMYRSGD 725
IY+ G+A GYL D+LT+++FLK F+ T NA RMY++GD
Sbjct: 1951 IYIGGAGVARGYLNRDDLTAERFLKDPFS--------TAPNA-----------RMYKTGD 1991
Query: 726 LGRYLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRENITLVRRDKDEEPTLV 785
LGRYLP GN+E GR DDQ+KIRGFRIELGEI L++ PN++E + L R D + LV
Sbjct: 1992 LGRYLPDGNIEYLGRNDDQVKIRGFRIELGEIEAKLAQAPNIKETVVLAREDVPGDKRLV 2051
Query: 786 AYIVPQGLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKTKLPSYAIPSVIVPLHKM 845
AY T+ + + L R +L+ +LP+Y +P V L +
Sbjct: 2052 AYF-------------TQHSPVDIEAL----------RTHLQAQLPAYMVPVAYVRLEAL 2088
Query: 846 PLNPNGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERDIRDIWLRIIPHATDVNK 905
PL PNGK+D+ ALP PD + G + E + IW ++ V +
Sbjct: 2089 PLTPNGKLDRKALPAPDLDAVIT-------RGYEAPQGEVEISLAQIWQDLL-KVERVGR 2140
Query: 906 KASFFDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGLAKEIERMKSGEMISVMD 965
FF++GGHS+LA LI +R + ++ + ++FS+PT+ LA + SG + V D
Sbjct: 2141 HDHFFELGGHSLLAVSLIGRMR-QLGLSADVRVLFSQPTLAALAAAV---GSGGEVVVPD 2196
Query: 966 IG 967
G
Sbjct: 2197 NG 2198
Score = 236 bits (603), Expect = 7e-65
Identities = 195/656 (29%), Positives = 305/656 (46%), Gaps = 113/656 (17%)
Query: 314 TYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPP 373
+YR+++E +N AH+L K G+E V + R +D+VV ++ +LKAG + +DPAYP
Sbjct: 577 SYRELNERANRQAHYLRKQGVEPDSRVAICVERSIDMVVGLLAILKAGGGYVPLDPAYPL 636
Query: 374 ARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLEL--KTYVPALKLAKDGSLTGGSV 431
R I Y+ LED+ +P V +LEL +P + L + G SV
Sbjct: 637 DR-IAYM----------LEDS---APAAVLTQTATLELLAAASMPVINL-ESGDWQDESV 681
Query: 432 SKGADDILQHVLHLKSEQTGVVVGPDSTPTLSFTSGSEGIPKGVKGRHFSLAYYFDWMAQ 491
V L S V+ +TSGS G+PKGV H + + W +
Sbjct: 682 QN------PEVTELTSSHLAYVI---------YTSGSTGLPKGVMIEHRNTVNFLTWAHR 726
Query: 492 EFNLSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPTAEDIGTPGQLAQWANKYKVTV 551
F+ + + + D + F PL G S+ V T L ++ +T+
Sbjct: 727 SFDAQTLSKTLFSTSLNFDLAVYECFAPLTSGGSIEVVT-------NVLELQQGEHDITL 779
Query: 552 THLTPAMGQLLAAQAD--EPIPSLHHAFFVGDILTKRDCLRLQVLANNVNVVNMYGTTET 609
+ P+ + L E + +++ A G+ L + L + N+YG +ET
Sbjct: 780 INTVPSALKALLESGGLGEGVDTVNVA---GEALKRSLVETLFEQTQVKRLCNLYGPSET 836
Query: 610 QRSVSYFVVPARSQDQTFLESQKDVIPA--GRGMKNVQLLVINRFDTNKICGIGEVGEIY 667
S+ + +++D A G+ + N Q +++ + + +G GEIY
Sbjct: 837 TTYSSWVSM-----------AREDGFAAHIGKPVANTQFYLLD--EHKQPVPLGVPGEIY 883
Query: 668 LRAGGLAEGYLGNDELTSKKFLKSWFADPSKFVDRTPENAPWKPYWFGIRDRMYRSGDLG 727
+ G+A GYL D+LT+++FLK F+ T NA RMYR+GDLG
Sbjct: 884 IGGAGVARGYLNRDDLTAERFLKDPFS--------TAPNA-----------RMYRTGDLG 924
Query: 728 RYLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRENITLVRRDKDEEPTLVAY 787
RYLP GN+E GR DDQ+KIRGFRIELGEI LS+H ++E + + R D + LVAY
Sbjct: 925 RYLPDGNIEYLGRNDDQVKIRGFRIELGEIEAKLSQHDALKEAVVVAREDVPGDKRLVAY 984
Query: 788 IVPQGLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKTKLPSYAIPSVIVPLHKMPL 847
T+ + I +R +L+ +LP+Y +P V L +PL
Sbjct: 985 F-------------TQHSPVD----------IETLRTHLQAQLPAYMVPVAYVRLEALPL 1021
Query: 848 NPNGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERDIRDIWLRIIPHATDVNKKA 907
PNGK+D+ ALP PD + G + TE + IW ++ V +
Sbjct: 1022 TPNGKLDRKALPAPDLDAVIT-------RGYEAPQGETETTLAQIWQDLL-KVERVGRHD 1073
Query: 908 SFFDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGLAKEIERMKSGEMISV 963
FF++GGHS+LA LI +R+ ++ + ++F +PT+ LA + SG+ ISV
Sbjct: 1074 HFFELGGHSLLAVTLIERMRQA-GLSADVRVLFGQPTLAALAAAV---GSGKEISV 1125
Score = 229 bits (585), Expect = 8e-63
Identities = 255/980 (26%), Positives = 405/980 (41%), Gaps = 156/980 (15%)
Query: 30 LPTDYSRPVPSRLVEAVFERTLPEDAKTALIKVYVAAQAKGILVTPFNILLTIFIILVSR 89
LPTD +RP A D TA +K +Q G VT + ++ + L+SR
Sbjct: 6737 LPTDRARPAQQDYAGATLPVVFDADL-TASLKAL--SQRHG--VTLYMTMMAAWGALLSR 6791
Query: 90 MTGDEDISIGT--------SSENAIPFVLRTFI-------QPSDSFLDLLAKVCDLEKEG 134
++G +++ IG+ E I F + T P+ L K L +
Sbjct: 6792 LSGQDEVVIGSPVANRTRSEVEGLIGFFVNTLAVRIDLSGAPTVETLLARVKAQALGAQA 6851
Query: 135 SSDAVDFSDLINFLNAKLSKKDDPRKTLVHLRFYNAPDAPSENFLSTTGLDVDLTVLVSV 194
D + F ++ L P ++L H + A LS D VL +
Sbjct: 6852 HQD-LPFEQVVEVLK--------PVRSLAHSPLFQA-------MLSWQTHDNTELVLGDL 6895
Query: 195 KKPSDQLTSLRSQFTFPDLQLKLIYNQL---------LFSESRVNIVA---DQLLKLVVS 242
K S ++F L+L I Q+ LF E V +LL+ +V+
Sbjct: 6896 KLEGLGAASNVAKFDV-SLELSEIQGQMFGALEYATALFDEGTVQRYLGYFQRLLRAMVA 6954
Query: 243 ASKDVTGPIGALDLMTPTQMNVLPDPTVDLDWSGYRGAIQDIFASNAAKFPDRECIVVTP 302
+ + I LD + ++L I +F + PD
Sbjct: 6955 SDQTRIERIALLD--NAEREHLLAGLNATQAAYPREQTIHQLFEAQVEARPD-------- 7004
Query: 303 SVTIDAPVTSYTYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGA 362
++ + +Y +++ +N +AHHL+ GI D V + RG D+++ ++ +LKAGA
Sbjct: 7005 AIAVAFGDERLSYAELNRQANQMAHHLIGLGIRPDDRVAICVERGADMLIGLLAILKAGA 7064
Query: 363 TFSVIDPAYPPARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKTYVPALKLAK 422
+ +DPA+P R L+ + P AL+ G L + + VP + L +
Sbjct: 7065 GYVPLDPAFPAERLAYMLADSAPVALL---SQGELLDRLPAFT---------VPVVLLDR 7112
Query: 423 DGSLTGGSVSKGADDILQHVLHLKSEQTGVVVGPDSTPTLSFTSGSEGIPKGVKGRHFSL 482
+ G ++ G DD +G + +TSGS G PKGV H L
Sbjct: 7113 AERVRCG-IAAGRDD----------NPLVASLGVRHLAYVIYTSGSTGNPKGVMIEHRGL 7161
Query: 483 AYYFDWMAQEFNLSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPTAEDIGTPGQLAQ 542
Y + F L+ D + + D +IF L GA+L PT + G+ Q
Sbjct: 7162 VNYCVDAVRLFGLTPDDTVLQQNTLNFDLSVEEIFPALLAGATL-APTRQLFGSAE--LQ 7218
Query: 543 WANKYKVTVTHLTPAMGQLLAA-------QADEPIPSLHHAFFVGDILTKRDCLRLQ--- 592
+ T HLT A LAA QA + + + GD L+ + +LQ
Sbjct: 7219 QNGDIRPTFLHLTAAHWHTLAAEWHNSPAQARDYLRDVRLINVTGDALSTQ---KLQMWD 7275
Query: 593 -VLANNVNVVNMYGTTETQRSVSYFVVPARSQDQTFLESQKDVIPAGRGMKNVQLLVINR 651
V + +VN YG TE S + A + ++ GR M N ++ +++
Sbjct: 7276 AVRPAHTRLVNTYGPTEATVSCT-----AAYMEHDAVDGILGNASIGRPMANTRIYLLDA 7330
Query: 652 FDTNKICGIGEVGEIYLRAGGLAEGYLGNDELTSKKFLKSWFADPSKFVDRTPENAPWKP 711
+ G GEI++ G+A GYL D + +++FL F+D
Sbjct: 7331 H--RQPVPFGVAGEIFIGGDGVARGYLNLDAINAERFLADPFSDQPDA------------ 7376
Query: 712 YWFGIRDRMYRSGDLGRYLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRENI 771
R+Y++GDL RY P G +E GR D Q+K+RGFRIELGEI L V+E
Sbjct: 7377 -------RLYKTGDLARYRPDGRLEYLGRNDFQVKVRGFRIELGEIEARLGDCAGVKEAA 7429
Query: 772 TLVRRDKDEEPTLVAYIVPQ-GLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKTKL 830
+VR D + LVAY+VPQ G+N + +R L +L
Sbjct: 7430 VIVREDTPGDKRLVAYVVPQAGVNLN----------------------AASLRAQLAVQL 7467
Query: 831 PSYAIPSVIVPLHKMPLNPNGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERDIR 890
Y +P V L +PL PN K+D+ ALP PD A ASR+H + TE +
Sbjct: 7468 AEYMLPGAFVSLAALPLTPNRKLDRQALPAPDAE--AYASRTH-----EAPQGHTEIVLA 7520
Query: 891 DIWLRIIPHATDVNKKASFFDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGLAK 950
IW ++ V + FF++GGHS+L RLI ++R++ V + L VF++P + LA+
Sbjct: 7521 QIWQSLL-KVESVGRHDHFFELGGHSLLVMRLIAQVREQLGVELNLRDVFAQPQLSALAQ 7579
Query: 951 EIERMKSGEMISVMDIGKEE 970
+ + ++ + +E+
Sbjct: 7580 VLSQAAHSTRPGIVPVSREQ 7599
Score = 220 bits (561), Expect = 5e-60
Identities = 200/739 (27%), Positives = 320/739 (43%), Gaps = 114/739 (15%)
Query: 229 VNIVADQLLKLVVSASKDVTGPIGALDLMTPTQMN-VLPDPTVDLDWSGYRGAIQDIFAS 287
+NI + L++ + A + P+ +L ++ P + +L D + +F +
Sbjct: 2641 MNIALEHLVEALEQAPQT---PLHSLSILPPAERRQLLVDFNATTRSYPQDRTVHGLFEA 2697
Query: 288 NAAKFPDRECIVVTPSVTIDAPVTSYTYRQIDESSNILAHHLVKNGIERGDVVMVYAYRG 347
A + P+ +V S TY +++ +N L HL+ G++ GD V + R
Sbjct: 2698 RAKELPEATAVVHNGH--------SLTYAELNRRANCLTRHLIGLGVQPGDRVAILLERS 2749
Query: 348 VDLVVAVMGVLKAGATFSVIDPAYPPARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVE- 406
V L+V+ + +LK A + +D + P RQ + + R L+ L V P + V+
Sbjct: 2750 VGLLVSQLAILKCAAVYVPLDVSAPLERQDFMVRDSGARTLLTLSAMAV--PEGIARVDL 2807
Query: 407 KSLELKTYVPALKLAKDGSLTGGSVSKGADDILQHVLHLKSEQTGVVVGPDSTPTLSFTS 466
S+ L+T H L L + +S + +TS
Sbjct: 2808 DSVVLETEA------------------------DHDLRL-------IQSTESVAYIMYTS 2836
Query: 467 GSEGIPKGVKGRHFSL-AYYFDWMAQEFNLSESDRFTMLSGIAHDPIQRDIFTPLFLGAS 525
GS G PKGV H ++ + EFN DR S A D D++ PL G
Sbjct: 2837 GSTGTPKGVLVPHRAINRLVINNGYAEFNAQ--DRVAFASNPAFDASTLDVWAPLLNGGC 2894
Query: 526 LIVPTAEDIGTPGQLAQWANKYKVTVTHLTPAMGQLLAAQADEPIPSLHHAFFVGDILTK 585
++V + + T + A + V+V +T + AA L + GD+L
Sbjct: 2895 VVVVDQDVLLTQERFAALLQEQSVSVLWMTAGLFHQYAAGLMSVFAQLRYLIVGGDVLDP 2954
Query: 586 RDCLRLQVLANNVNVVNMYGTTETQRSVSYFVVPARSQDQTFLESQKDVIPAGRGMKNVQ 645
R+ +++N YG TE + + + + IP GR + N +
Sbjct: 2955 AVIGRVLKEGAPQHLLNGYGPTEATTFTTTHEIKSVGEGG---------IPIGRPIGNTR 3005
Query: 646 LLVINRFDTNKICGIGEVGEIYLRAGGLAEGYLGNDELTSKKFLKSWF-ADPSKFVDRTP 704
+ V++ + IG GE+Y+ G+A+GYL EL+++KF+ F ADP
Sbjct: 3006 VYVLDA--NRQPVPIGVAGELYIGGDGVAKGYLNRPELSAEKFVADPFNADPGAL----- 3058
Query: 705 ENAPWKPYWFGIRDRMYRSGDLGRYLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRH 764
+YR+GDL R+ G V+ GR DDQ+KIRGFRIELGEI L
Sbjct: 3059 ---------------LYRTGDLARWRADGAVDYLGRNDDQVKIRGFRIELGEIEARLGEC 3103
Query: 765 PNVRENITLVRRDKDEEPTLVAYIVPQGLNKDDFDSATESEDIVVNGLKKYRKLIHDIRE 824
P V++ + L R+D LV Y++P+ T S +H +RE
Sbjct: 3104 PGVKDAVVLARQDDAGPKRLVGYVIPE-------PGVTLS--------------VHALRE 3142
Query: 825 YLKTKLPSYAIPSVIVPLHKMPLNPNGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTA 884
L + L Y +PS V L PL NGK+D+ LP PD A ASR + + L
Sbjct: 3143 QLSSVLADYMLPSAFVVLPAFPLTANGKLDRRELPAPDAE--AYASREY-----EAPLGK 3195
Query: 885 TERDIRDIWLRIIPHATDVNKKASFFDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPT 944
E+ + IW ++ V + FF++GGHS+LA LI +R+ ++ + ++FS+PT
Sbjct: 3196 VEQTLAQIWADVL-KVEQVGRHDHFFELGGHSLLAVTLIERMRQA-GLSADVRVLFSQPT 3253
Query: 945 IEGLAKEIERMKSGEMISV 963
+ LA I SG +SV
Sbjct: 3254 LAALAAAI---GSGREVSV 3269
Score = 202 bits (513), Expect = 2e-54
Identities = 177/644 (27%), Positives = 279/644 (43%), Gaps = 106/644 (16%)
Query: 314 TYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPP 373
TYR++ E +N LAH L++ G++ G V + R +L+++ + +LK A + +D P
Sbjct: 9168 TYRELHERANHLAHCLLERGVQPGSRVAIMLNRSFELIISELAILKCAAVYVPLDHHAPE 9227
Query: 374 ARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKTYVPALKLAKDGSLTGGSVSK 433
RQ L + A VL T + L +P L L
Sbjct: 9228 ERQRFMLQ----------DSAAVLVLTT-----RDRALPEDMPRLDL------------- 9259
Query: 434 GADDILQHVLHLKSEQTGVVVGPDS-TPT-LSFTSGSEGIPKGVKGRHFSLAY------Y 485
D LQ + ++ + V G S TP + +TSGS G PKGV H ++ Y
Sbjct: 9260 ---DTLQLI---QAARPAVFPGQTSDTPAYIMYTSGSTGHPKGVIVPHLAIGRLAINNGY 9313
Query: 486 FDWMAQEFNLSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPTAEDIGTPGQLAQWAN 545
D+ Q DR S A D +++ L G +++ E + +P Q AQ
Sbjct: 9314 ADFGVQ-------DRVAFASNPAFDASTMEVWGALLNGGRVVIIDHETLLSPTQFAQALT 9366
Query: 546 KYKVTVTHLTPAMGQLLAAQADEPIPSLHHAFFVGDILTKRDCLRLQVLANNVNVVNMYG 605
+ +V+V +T AM + + L G+ RL A ++ +V+ YG
Sbjct: 9367 EAEVSVLFVTTAMFNQYLVLIPDALAGLRILLCGGERGDPASFRRLLAHAPSLRLVHCYG 9426
Query: 606 TTETQRSVSYFVVPARSQDQTFLESQKDVIPAGRGMKNVQLLVINRFDTNKICGIGEVGE 665
TET + VV A T +P GR + N ++ +++ + +G GE
Sbjct: 9427 PTETTTFATTHVVSAVPHGATS-------VPIGRPIANTRVYILDA--ARQPVPVGVAGE 9477
Query: 666 IYLRAGGLAEGYLGNDELTSKKFLKSWFADPSKFVDRTPENAPWKPYWFGIRDRMYRSGD 725
I++ G+A GYL +LT ++F+ F+D R+YRSGD
Sbjct: 9478 IHIGGVGVALGYLNRPDLTEERFIADPFSDSGSA-------------------RLYRSGD 9518
Query: 726 LGRYLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRENITLVRRDKDEEPTLV 785
LGR+LP G +E R D Q+KIRGFRIELGEI L VRE + + R D + L+
Sbjct: 9519 LGRWLPDGTIEYLDRNDGQVKIRGFRIELGEIEARLHECHGVREALVVAREDSPGDKCLI 9578
Query: 786 AYIVPQGLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKTKLPSYAIPSVIVPLHKM 845
AY + N D D+ +R L++ LP Y IP+ V L +
Sbjct: 9579 AYYT-EHENGGDLDADV-------------------LRAQLRSVLPEYMIPAAYVRLTAL 9618
Query: 846 PLNPNGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERDIRDIWLRIIPHATDVNK 905
PL NGK++ ALP P+ + + ++ TL D+ + + +
Sbjct: 9619 PLTLNGKVNTGALPVPEMHAYGSQDYEAPQGDLEVTLAGLWADVLKL--------EQIGR 9670
Query: 906 KASFFDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGLA 949
+FF++GGHS+LA RL+ L + + V L +F ++ +A
Sbjct: 9671 HDNFFELGGHSLLAIRLV-GLLAQANLTVSLAELFQHESVASMA 9713
Lambda K H
0.318 0.136 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 30,225
Number of extensions: 1368
Number of successful extensions: 65
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 14
Length of query: 1419
Length of database: 10,307
Length adjustment: 64
Effective length of query: 1355
Effective length of database: 10,243
Effective search space: 13879265
Effective search space used: 13879265
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 67 (30.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory