Align L-2-aminoadipate reductase; Alpha-aminoadipate reductase; Alpha-AR; L-aminoadipate-semialdehyde dehydrogenase; EC 1.2.1.31; EC 1.2.1.95 (characterized)
to candidate AO356_28210 AO356_28210 hypothetical protein
Query= SwissProt::P40976
(1419 letters)
>FitnessBrowser__pseudo5_N2C3_1:AO356_28210
Length = 10307
Score = 252 bits (644), Expect = 1e-69
Identities = 191/660 (28%), Positives = 316/660 (47%), Gaps = 102/660 (15%)
Query: 315 YRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPPA 374
YR+++ SN LAH+L K G+E V + RG+++VV ++ +LKAG + +DPAYP
Sbjct: 5968 YRELNGLSNRLAHYLRKQGVEPDSRVAICVERGIEMVVGLLAILKAGGGYVPLDPAYPLD 6027
Query: 375 RQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKTYVPALKLAKDGSLTGGSVSKG 434
R L + P A++V + ++L +T +P + L K G +
Sbjct: 6028 RIAYMLEDSAPAAILV------------QGTTRNLLGETAIPVIDLDK-----GIWQDEA 6070
Query: 435 ADDILQHVLHLKSEQTGVVVGPDSTPTLSFTSGSEGIPKGVKGRHFSLAYYFDWMAQEFN 494
A + V L S V+ +TSGS G+PKGV H + + W + F+
Sbjct: 6071 APN--PQVPGLSSSHLAYVI---------YTSGSTGLPKGVMIEHRNTVNFLTWAHRSFD 6119
Query: 495 LSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPTAEDIGTPGQLAQWANKYKVTVTHL 554
+ + + D + F PL G S+ V T L ++ +T+ +
Sbjct: 6120 AQILSKTLFSTSLNFDLAVYECFAPLTSGGSIEVVT-------NVLELQQGEHDITLINT 6172
Query: 555 TPAMGQLLAAQAD--EPIPSLHHAFFVGDILTKRDCLRLQVLANNVNVVNMYGTTETQRS 612
P+ + L E + +++ A G+ L + L + N+YG +ET
Sbjct: 6173 VPSALKALLESGGLGEGVDTVNVA---GEALKRSLVETLFEQTQVKRLCNLYGPSETTTY 6229
Query: 613 VSYFVVPARSQDQTFLESQKDVIPA--GRGMKNVQLLVINRFDTNKICGIGEVGEIYLRA 670
S+ + +++D A G+ + N Q +++ + + +G GEIY+
Sbjct: 6230 SSWVSM-----------AREDGFAAHIGKPVANTQFYLLD--EHKQPVPLGVPGEIYIGG 6276
Query: 671 GGLAEGYLGNDELTSKKFLKSWFADPSKFVDRTPENAPWKPYWFGIRDRMYRSGDLGRYL 730
G+A GYL D+LT+++FLK+ F+ T NA RMYR+GDLGRYL
Sbjct: 6277 AGVARGYLNRDDLTAERFLKNPFS--------TAPNA-----------RMYRTGDLGRYL 6317
Query: 731 PTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRENITLVRRDKDEEPTLVAYIVP 790
P GN+E GR DDQ+KIRGFRIELGEI L++H ++E + + R D + LVAY
Sbjct: 6318 PDGNIEYLGRNDDQVKIRGFRIELGEIEAKLAQHDALKEAVVVAREDVPGDKRLVAY--- 6374
Query: 791 QGLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKTKLPSYAIPSVIVPLHKMPLNPN 850
F + E + + L R +L+T+LP+Y +P+V V L +PL PN
Sbjct: 6375 -------FTQHSPDETVEIEAL----------RTHLQTQLPAYMVPAVYVRLDALPLTPN 6417
Query: 851 GKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERDIRDIWLRIIPHATDVNKKASFF 910
GK+D+ ALP PD + + + V+ TL +D+ D+ V + FF
Sbjct: 6418 GKLDRKALPAPDLDAVISRGYEAPQGEVETTLAQIWQDLLDL--------QQVGRHDQFF 6469
Query: 911 DIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGLAKEIERMKSGEMISVMDIGKEE 970
++GGHS+LA +LI ++R + V + L +F++P + GLA+ + + ++ + +++
Sbjct: 6470 ELGGHSLLAIQLISQVRLRLGVELGLTELFAQPQLMGLAQAVALAGRSTLPEIVRVSRKD 6529
Score = 252 bits (644), Expect = 1e-69
Identities = 248/963 (25%), Positives = 403/963 (41%), Gaps = 143/963 (14%)
Query: 20 ERLKSQTISH------LPTDYSRPVPS----RLVEAVFERTLPEDAKTALIKVYVAAQAK 69
ER QT++ LPTD RP V V + TL + K +Q
Sbjct: 7791 ERFWRQTLADAPALLTLPTDRPRPAQQDHAGAAVGIVLDETLTQGLKAL-------SQRH 7843
Query: 70 GILVTPFNILLTIFIILVSRMTGDEDISIGTSSENAIPFVLRTFIQPSDSFLDLLAKVCD 129
G T F ++ + ++SR++G D+ IGT N + + I F++ LA D
Sbjct: 7844 G--ATLFMTVMAAWATVLSRLSGQHDVVIGTPVANRMQAEVEGLI---GLFVNTLALRVD 7898
Query: 130 LEKEGSSDAVDFSDLINFLNAKLSKKDD---------PRKTLVHLRFYNAPDAPSENFLS 180
+ E + A+ + L+A+ + P ++L H + A + N
Sbjct: 7899 VADELTVQALLQQVKVRTLDAQAHQDLPFEQVVEVVRPLRSLSHSPVFQAMLSWQNN--E 7956
Query: 181 TTGLDVDLTVLVSVKKPSDQLTSLRSQF-TFPDLQL-------KLIYNQLLFSESRVNIV 232
TGL++ L + +TS ++F D+ L L Y LF + +
Sbjct: 7957 ATGLELGDMSLQGLG-----VTSRTAKFDVLLDMALIDGRLFGALEYATALFDSATMERY 8011
Query: 233 ADQLLKLVVSASKDVTGPIGALDLMTPTQMNVLPDPTVDLDWSGYRGAI--QDIFASNAA 290
L ++ + D + + L+ + L + T++ Y + +F +
Sbjct: 8012 LGYLESVLRAMVADEQTLVAQIPLLGDNERQNLLE-TLNATAVDYPQGLTLHGLFEARVV 8070
Query: 291 KFPDRECIVVTPSVTIDAPVTSYTYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDL 350
PD +V + + +Y Q++E +N +AH L++ GI D V + A R +D+
Sbjct: 8071 ASPD--------AVAVVSGEAQLSYAQLNERANQIAHRLLELGIRPDDRVAICADRSLDM 8122
Query: 351 VVAVMGVLKAGATFSVIDPAYPPARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLE 410
V ++G+LKAGA + +DP YP R L + P +VVL + + SL
Sbjct: 8123 VAGLLGILKAGAAYVPLDPDYPHERLAYMLENSAP--MVVLTQRAL---------QASLP 8171
Query: 411 LKTYVPALKLAKDGSLTGGSVSKGADDILQHVLHLKSEQTGVVVGPDSTPTLSFTSGSEG 470
L G T + + + + H HL + +TSGS G
Sbjct: 8172 ATQARTLLLDEAQGFSTQPTDNPQVEGL--HAHHLAY--------------VIYTSGSTG 8215
Query: 471 IPKGVKGRHFSLAYYFDWMAQEFNLSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPT 530
+PKGV H + WM E+ L+ +D + + D + F PLF GA L++
Sbjct: 8216 LPKGVMNEHGGVVNRLLWMQDEYGLTATDAVLQKTPFSFDVSVWEFFWPLFSGARLVMAR 8275
Query: 531 AEDIGTPGQLAQWANKYKVTVTHLTPAMGQLLAAQAD-EPIPSLHHAFFVGDILTKRDCL 589
P L + +T H P+M + A + L G+ L
Sbjct: 8276 PGGHRDPAYLREVIQAQGITTLHFVPSMLDVFLAHGEASQCAGLVRVMCSGEALPGHLVR 8335
Query: 590 RLQVLANNVNVVNMYGTTETQRSVSYFVVPARSQDQTFLESQKDVIPAGRGMKNVQLLVI 649
R + + + N+YG TE V+ + D P G+ + N ++ ++
Sbjct: 8336 RFKAQLPHTGLYNLYGPTEAAVDVTAWDCAGTDTP--------DSTPIGKPIANTRIYLL 8387
Query: 650 NRFDTNKICGIGEVGEIYLRAGGLAEGYLGNDELTSKKFLKSWFADPSKFVDRTPENAPW 709
+ G+ GEIY+ +A GYL ELT ++FL+ F+ + NA
Sbjct: 8388 DGHQQPVPMGVA--GEIYIGGVQVARGYLNRPELTVERFLRDLFSRAA--------NA-- 8435
Query: 710 KPYWFGIRDRMYRSGDLGRYLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRE 769
RMYR+GDLGRYLP GN+E GR DDQ+KIRGFRIELGEI L++H ++E
Sbjct: 8436 ---------RMYRTGDLGRYLPDGNIEYLGRNDDQVKIRGFRIELGEIEAKLAQHEAIKE 8486
Query: 770 NITLVRRDKDEEPTLVAYIVPQGLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKTK 829
+ L D LVAY G ++ I ++R L+ +
Sbjct: 8487 AVVLALEDVPGAKRLVAYFTVHG--------------------DEHSVEIENLRTQLQAQ 8526
Query: 830 LPSYAIPSVIVPLHKMPLNPNGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERDI 889
LP Y +P+ V L +PL PNGK+D+ ALP PD + + + V+ TL
Sbjct: 8527 LPEYMVPAAYVRLQALPLTPNGKLDRKALPAPDLASVITREYEAPQGAVETTLA------ 8580
Query: 890 RDIWLRIIPHATDVNKKASFFDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGLA 949
+W ++ V + FF++GGHS+LA LI +R+ ++ + ++F +PT+ LA
Sbjct: 8581 -QLWAELL-KVEKVGRHDHFFELGGHSLLAVTLIERMRQA-GLSADVRVLFGQPTLAALA 8637
Query: 950 KEI 952
+
Sbjct: 8638 AAV 8640
Score = 246 bits (629), Expect = 7e-68
Identities = 216/735 (29%), Positives = 331/735 (45%), Gaps = 102/735 (13%)
Query: 225 SESRVNIVADQLLKLVVSASKDVTGPIGALDLMTPTQMNVLPDPTVDLDWSGYRGAIQDI 284
+E N V + LV + + P+ L ++ P + L + W+G A
Sbjct: 4798 AERVCNYVQCVVENLVQALEQSPLAPLSGLKILPPAERRQLLE-----SWNGTGVA---- 4848
Query: 285 FASNAAKFPDRECIVVTP--SVTIDAPVTSYTYRQIDESSNILAHHLVKNGIERGDVVMV 342
FA +A E ++ + + TY +++ +N +AH L+ GI D V +
Sbjct: 4849 FAQDALIHAQFEVWAAAQPEAMAVMFEERTLTYSELNARANQVAHRLLAQGIRPDDRVAI 4908
Query: 343 YAYRGVDLVVAVMGVLKAGATFSVIDPAYPPARQIIYLSVAKPRALVVLEDAGVLSPTVV 402
RG+D++V ++G+LKAGA + +DPAYP R L + P AL+
Sbjct: 4909 CVERGLDMIVGLLGILKAGAGYVPVDPAYPTERIAYMLRDSMPVALLA------------ 4956
Query: 403 EYVEKSLELKTYVPALKLAKDGSLTGGSVSKGADDILQHVLHLKSEQTGVVVGPDSTPTL 462
L +P L+L DGS SV+ V L S V+
Sbjct: 4957 HAATHRLLAGVSMPVLQL-DDGSWQDESVAN------PQVPGLDSSHLAYVI-------- 5001
Query: 463 SFTSGSEGIPKGVKGRHFSLAYYFDWMAQEFNLSESDRFTMLSGIAHDPIQRDIFTPLFL 522
+TSGS G+PKGV H ++A F F+ + D + + A D +I+ L
Sbjct: 5002 -YTSGSTGLPKGVMVEHRNVARLFSATQPWFDFGQQDVWALFHSFAFDFSVWEIWGALTH 5060
Query: 523 GASLIVPTAEDIGTPGQLAQWANKYKVTVTHLTP-AMGQLLAAQADEPIP-SLHHAFFVG 580
G L+V +P + VTV + TP A QL+ AQ + + SL F G
Sbjct: 5061 GGRLLVVPQLVSRSPQDCYALLCETGVTVLNQTPSAFRQLIVAQGESDLRHSLRQVIFGG 5120
Query: 581 DILTKRDCLRLQVLANNVN--VVNMYGTTETQRSVSYFVVPARSQDQTFLESQKDVIPAG 638
+ L N +VNMYG TET V+Y + A T V P G
Sbjct: 5121 EALETGMLKPWYARVGNAGTQLVNMYGITETTVHVTYRALEAADAQST------GVSPIG 5174
Query: 639 RGMKNVQLLVINRFDTNKICGIGEVGEIYLRAGGLAEGYLGNDELTSKKFLKSWFADPSK 698
+ + ++QL V++ + +G VGE+Y+ G++ GYL DELT+++FL + F+
Sbjct: 5175 KRIPDLQLYVLDA--QREPAPVGVVGEMYVGGAGVSRGYLNRDELTAERFLDNPFS---- 5228
Query: 699 FVDRTPE-NAPWKPYWFGIRDRMYRSGDLGRYLPTGNVECSGRADDQIKIRGFRIELGEI 757
PE NA RMYR+GDLGR+L G++E GR DDQ+KIRGFRIELGEI
Sbjct: 5229 -----PEPNA-----------RMYRTGDLGRWLADGSIEYLGRNDDQVKIRGFRIELGEI 5272
Query: 758 NTHLSRHPNVRENITLVRRDKDEEPTLVAYIVPQGLNKDDFDSATESEDIVVNGLKKYRK 817
L+ V E + + R D+ + LVAY++ ATE +
Sbjct: 5273 EATLAACEGVSEAVVIAREDEPGDKRLVAYVI-----------ATEGA----------QP 5311
Query: 818 LIHDIREYLKTKLPSYAIPSVIVPLHKMPLNPNGKIDKPALPFPDTSQLAAASRSHSKHG 877
D+R L L + +PS V L PL NGK+D+ ALP PD S + + +
Sbjct: 5312 SAVDLRTQLLRSLAEHMVPSAFVILDAFPLTTNGKLDRKALPAPDQSAVISREYEAPQGE 5371
Query: 878 VDETLTATERDIRDIWLRIIPHATDVNKKASFFDIGGHSILATRLIFELRKKFAVNVPLG 937
V+ T+ +D+ + V + +FF++GGHS+LA +LI E ++ + +
Sbjct: 5372 VETTIARIWQDLLKL--------GRVGRHDNFFELGGHSLLAVKLI-ERMRQVDLAADVR 5422
Query: 938 LVFSEPTIEGLAKEI 952
++F +PT+ LA +
Sbjct: 5423 VLFGQPTLAALAAAV 5437
Score = 241 bits (616), Expect = 2e-66
Identities = 213/724 (29%), Positives = 325/724 (44%), Gaps = 99/724 (13%)
Query: 236 LLKLVVSASKDVTGPIGALDLMTPTQMNVLPDP--TVDLDWSGYRGAIQDIFASNAAKFP 293
L LV++ + P+ AL ++ + L + T D+ ++ I F AA P
Sbjct: 3719 LENLVMALEQAPQTPLHALAILPSAERRQLLETWNTTDVTYAD-DALIHRQFEGWAASQP 3777
Query: 294 DRECIVVTPSVTIDAPVTSYTYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVA 353
D +V + TY +++ +N LAH LV G+ D V + RG+D+++
Sbjct: 3778 DTVAVVHEEQ--------ALTYGELNARANQLAHSLVALGVRPDDRVAICVERGLDMIIG 3829
Query: 354 VMGVLKAGATFSVIDPAYPPARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKT 413
++G+LKAGA + +DP YPP R L + P A+V P +
Sbjct: 3830 LLGILKAGAGYVPLDPTYPPERIAYMLHDSSPVAVVTQSQWRDSLPVLP----------- 3878
Query: 414 YVPALKLAKDGSLTGGSVSKGADDILQHVLHLKSEQTGVVVGPDSTPTLSFTSGSEGIPK 473
VP L L + + D+ L + V+ +TSGS G+PK
Sbjct: 3879 -VPTLVLDPTDAGEMNQQPRHNPDVAD----LNASHLAYVI---------YTSGSTGLPK 3924
Query: 474 GVKGRHFSLAYYFDWMAQEFNLSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPTAED 533
GV H ++A F F D + + A D +I+ L G L+V
Sbjct: 3925 GVMVEHRNVARLFSATQPWFEFGPQDVWALFHSFAFDFSVWEIWGALTHGGRLLVVPQLV 3984
Query: 534 IGTPGQLAQWANKYKVTVTHLTP-AMGQLLAAQADEPIP-SLHHAFFVGDILTKRDCLRL 591
+P + VT+ + TP A QL+AAQ + + SL F G+ L
Sbjct: 3985 SRSPQYCYALLCEAGVTILNQTPSAFRQLIAAQGESDLSHSLRQVIFGGEALETGILKPW 4044
Query: 592 QVLANNVN--VVNMYGTTETQRSVSYFVVPARSQDQTFLESQKDVIPAGRGMKNVQLLVI 649
N +VNMYG TET V+Y + A V P G+ + ++QL V+
Sbjct: 4045 YARQANARTQLVNMYGITETTVHVTYRPLDAADAQLA------GVSPIGKRIPDLQLYVL 4098
Query: 650 NRFDTNKICGIGEVGEIYLRAGGLAEGYLGNDELTSKKFLKSWFADPSKFVDRTPE-NAP 708
+ + +G GE+Y+ GG++ GYL DELT+++FL + F+ PE NA
Sbjct: 4099 DT--QREPVPVGVTGEMYVGGGGVSRGYLNRDELTAERFLDNPFS---------PEPNA- 4146
Query: 709 WKPYWFGIRDRMYRSGDLGRYLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVR 768
RMYR+GDLGR+L G++E GR DDQ+KIRGFRIELGEI L+ VR
Sbjct: 4147 ----------RMYRTGDLGRWLADGSIEYLGRNDDQVKIRGFRIELGEIEAKLAACNGVR 4196
Query: 769 ENITLVRRDKDEEPTLVAYIVPQGLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKT 828
E + + R+D + LVAY++ + + + SA E +R+ L
Sbjct: 4197 EAVVVARQDTPGDQRLVAYVIAE---EGEPPSAAE------------------LRDELLG 4235
Query: 829 KLPSYAIPSVIVPLHKMPLNPNGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERD 888
L Y +PS V L PL NGK+D+ ALP PD S + + V E
Sbjct: 4236 SLAEYMVPSAFVMLTVFPLTTNGKLDRKALPVPDQSSVVTREYEAPQGEV-------EIA 4288
Query: 889 IRDIWLRIIPHATDVNKKASFFDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGL 948
I IW ++ H V + FF++GGHS+LA +L+ E ++ + + ++F +PT+ L
Sbjct: 4289 IARIWQDLL-HVERVGRHDHFFEMGGHSLLAVKLV-ERMRQIDLAADVRVLFGQPTLAAL 4346
Query: 949 AKEI 952
A +
Sbjct: 4347 AAAV 4350
Score = 239 bits (610), Expect = 1e-65
Identities = 193/662 (29%), Positives = 307/662 (46%), Gaps = 117/662 (17%)
Query: 314 TYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPP 373
+Y +++E +N LAH+L K G++ V + RG+D+VV ++ +LKAG + +DPAYP
Sbjct: 1646 SYGELNERANRLAHYLRKQGVKPDSRVAICVERGIDMVVGLLAILKAGGGYVPLDPAYPL 1705
Query: 374 ARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKTYVPALKLAKDGSLTGGSVSK 433
R I Y+ A V+ +DA V L D S+ ++
Sbjct: 1706 DR-IAYMLEDSAPAAVLAQDATV----------------------GLLSDASMAVINLDS 1742
Query: 434 G--ADDILQH--VLHLKSEQTGVVVGPDSTPTLSFTSGSEGIPKGVKGRHFSLAYYFDWM 489
G D+ +Q+ V L S V+ +TSGS G+PKGV H + + W
Sbjct: 1743 GDWQDESVQNPEVTELTSSHLAYVI---------YTSGSTGLPKGVMIEHRNTVNFLTWA 1793
Query: 490 AQEFNLSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPTAEDIGTPGQLAQWANKYKV 549
+ F+ + + + D + F PL G S+ V T L ++ +
Sbjct: 1794 HRSFDSQTLAKTLFSTSLNFDLAVYECFAPLTSGGSIEVVT-------NVLELQQGEHDI 1846
Query: 550 TVTHLTPAMGQLLAAQAD--EPIPSLHHAFFVGDILTKRDCLRLQVLANNVNVVNMYGTT 607
T+ + P+ + L E + +++ A G+ L + L + N+YG +
Sbjct: 1847 TLINTVPSALKALLESGGLGEGVDTVNVA---GEALKRSLVETLFEQTQVKRLCNLYGPS 1903
Query: 608 ETQRSVSYFVVPARSQDQTFLESQKDVIPA--GRGMKNVQLLVINRFDTNKICGIGEVGE 665
ET S+ + +++D A G+ + N Q +++ + + +G GE
Sbjct: 1904 ETTTYSSWVSM-----------AREDGFAAHIGKPVANTQFYLLD--EHKQPVPLGVPGE 1950
Query: 666 IYLRAGGLAEGYLGNDELTSKKFLKSWFADPSKFVDRTPENAPWKPYWFGIRDRMYRSGD 725
IY+ G+A GYL D+LT+++FLK F+ T NA RMY++GD
Sbjct: 1951 IYIGGAGVARGYLNRDDLTAERFLKDPFS--------TAPNA-----------RMYKTGD 1991
Query: 726 LGRYLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRENITLVRRDKDEEPTLV 785
LGRYLP GN+E GR DDQ+KIRGFRIELGEI L++ PN++E + L R D + LV
Sbjct: 1992 LGRYLPDGNIEYLGRNDDQVKIRGFRIELGEIEAKLAQAPNIKETVVLAREDVPGDKRLV 2051
Query: 786 AYIVPQGLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKTKLPSYAIPSVIVPLHKM 845
AY T+ + + L R +L+ +LP+Y +P V L +
Sbjct: 2052 AYF-------------TQHSPVDIEAL----------RTHLQAQLPAYMVPVAYVRLEAL 2088
Query: 846 PLNPNGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERDIRDIWLRIIPHATDVNK 905
PL PNGK+D+ ALP PD + G + E + IW ++ V +
Sbjct: 2089 PLTPNGKLDRKALPAPDLDAVIT-------RGYEAPQGEVEISLAQIWQDLL-KVERVGR 2140
Query: 906 KASFFDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGLAKEIERMKSGEMISVMD 965
FF++GGHS+LA LI +R + ++ + ++FS+PT+ LA + SG + V D
Sbjct: 2141 HDHFFELGGHSLLAVSLIGRMR-QLGLSADVRVLFSQPTLAALAAAV---GSGGEVVVPD 2196
Query: 966 IG 967
G
Sbjct: 2197 NG 2198
Score = 236 bits (603), Expect = 7e-65
Identities = 195/656 (29%), Positives = 305/656 (46%), Gaps = 113/656 (17%)
Query: 314 TYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPP 373
+YR+++E +N AH+L K G+E V + R +D+VV ++ +LKAG + +DPAYP
Sbjct: 577 SYRELNERANRQAHYLRKQGVEPDSRVAICVERSIDMVVGLLAILKAGGGYVPLDPAYPL 636
Query: 374 ARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLEL--KTYVPALKLAKDGSLTGGSV 431
R I Y+ LED+ +P V +LEL +P + L + G SV
Sbjct: 637 DR-IAYM----------LEDS---APAAVLTQTATLELLAAASMPVINL-ESGDWQDESV 681
Query: 432 SKGADDILQHVLHLKSEQTGVVVGPDSTPTLSFTSGSEGIPKGVKGRHFSLAYYFDWMAQ 491
V L S V+ +TSGS G+PKGV H + + W +
Sbjct: 682 QN------PEVTELTSSHLAYVI---------YTSGSTGLPKGVMIEHRNTVNFLTWAHR 726
Query: 492 EFNLSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPTAEDIGTPGQLAQWANKYKVTV 551
F+ + + + D + F PL G S+ V T L ++ +T+
Sbjct: 727 SFDAQTLSKTLFSTSLNFDLAVYECFAPLTSGGSIEVVT-------NVLELQQGEHDITL 779
Query: 552 THLTPAMGQLLAAQAD--EPIPSLHHAFFVGDILTKRDCLRLQVLANNVNVVNMYGTTET 609
+ P+ + L E + +++ A G+ L + L + N+YG +ET
Sbjct: 780 INTVPSALKALLESGGLGEGVDTVNVA---GEALKRSLVETLFEQTQVKRLCNLYGPSET 836
Query: 610 QRSVSYFVVPARSQDQTFLESQKDVIPA--GRGMKNVQLLVINRFDTNKICGIGEVGEIY 667
S+ + +++D A G+ + N Q +++ + + +G GEIY
Sbjct: 837 TTYSSWVSM-----------AREDGFAAHIGKPVANTQFYLLD--EHKQPVPLGVPGEIY 883
Query: 668 LRAGGLAEGYLGNDELTSKKFLKSWFADPSKFVDRTPENAPWKPYWFGIRDRMYRSGDLG 727
+ G+A GYL D+LT+++FLK F+ T NA RMYR+GDLG
Sbjct: 884 IGGAGVARGYLNRDDLTAERFLKDPFS--------TAPNA-----------RMYRTGDLG 924
Query: 728 RYLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRENITLVRRDKDEEPTLVAY 787
RYLP GN+E GR DDQ+KIRGFRIELGEI LS+H ++E + + R D + LVAY
Sbjct: 925 RYLPDGNIEYLGRNDDQVKIRGFRIELGEIEAKLSQHDALKEAVVVAREDVPGDKRLVAY 984
Query: 788 IVPQGLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKTKLPSYAIPSVIVPLHKMPL 847
T+ + I +R +L+ +LP+Y +P V L +PL
Sbjct: 985 F-------------TQHSPVD----------IETLRTHLQAQLPAYMVPVAYVRLEALPL 1021
Query: 848 NPNGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERDIRDIWLRIIPHATDVNKKA 907
PNGK+D+ ALP PD + G + TE + IW ++ V +
Sbjct: 1022 TPNGKLDRKALPAPDLDAVIT-------RGYEAPQGETETTLAQIWQDLL-KVERVGRHD 1073
Query: 908 SFFDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGLAKEIERMKSGEMISV 963
FF++GGHS+LA LI +R+ ++ + ++F +PT+ LA + SG+ ISV
Sbjct: 1074 HFFELGGHSLLAVTLIERMRQA-GLSADVRVLFGQPTLAALAAAV---GSGKEISV 1125
Score = 229 bits (585), Expect = 8e-63
Identities = 255/980 (26%), Positives = 405/980 (41%), Gaps = 156/980 (15%)
Query: 30 LPTDYSRPVPSRLVEAVFERTLPEDAKTALIKVYVAAQAKGILVTPFNILLTIFIILVSR 89
LPTD +RP A D TA +K +Q G VT + ++ + L+SR
Sbjct: 6737 LPTDRARPAQQDYAGATLPVVFDADL-TASLKAL--SQRHG--VTLYMTMMAAWGALLSR 6791
Query: 90 MTGDEDISIGT--------SSENAIPFVLRTFI-------QPSDSFLDLLAKVCDLEKEG 134
++G +++ IG+ E I F + T P+ L K L +
Sbjct: 6792 LSGQDEVVIGSPVANRTRSEVEGLIGFFVNTLAVRIDLSGAPTVETLLARVKAQALGAQA 6851
Query: 135 SSDAVDFSDLINFLNAKLSKKDDPRKTLVHLRFYNAPDAPSENFLSTTGLDVDLTVLVSV 194
D + F ++ L P ++L H + A LS D VL +
Sbjct: 6852 HQD-LPFEQVVEVLK--------PVRSLAHSPLFQA-------MLSWQTHDNTELVLGDL 6895
Query: 195 KKPSDQLTSLRSQFTFPDLQLKLIYNQL---------LFSESRVNIVA---DQLLKLVVS 242
K S ++F L+L I Q+ LF E V +LL+ +V+
Sbjct: 6896 KLEGLGAASNVAKFDV-SLELSEIQGQMFGALEYATALFDEGTVQRYLGYFQRLLRAMVA 6954
Query: 243 ASKDVTGPIGALDLMTPTQMNVLPDPTVDLDWSGYRGAIQDIFASNAAKFPDRECIVVTP 302
+ + I LD + ++L I +F + PD
Sbjct: 6955 SDQTRIERIALLD--NAEREHLLAGLNATQAAYPREQTIHQLFEAQVEARPD-------- 7004
Query: 303 SVTIDAPVTSYTYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGA 362
++ + +Y +++ +N +AHHL+ GI D V + RG D+++ ++ +LKAGA
Sbjct: 7005 AIAVAFGDERLSYAELNRQANQMAHHLIGLGIRPDDRVAICVERGADMLIGLLAILKAGA 7064
Query: 363 TFSVIDPAYPPARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKTYVPALKLAK 422
+ +DPA+P R L+ + P AL+ G L + + VP + L +
Sbjct: 7065 GYVPLDPAFPAERLAYMLADSAPVALL---SQGELLDRLPAFT---------VPVVLLDR 7112
Query: 423 DGSLTGGSVSKGADDILQHVLHLKSEQTGVVVGPDSTPTLSFTSGSEGIPKGVKGRHFSL 482
+ G ++ G DD +G + +TSGS G PKGV H L
Sbjct: 7113 AERVRCG-IAAGRDD----------NPLVASLGVRHLAYVIYTSGSTGNPKGVMIEHRGL 7161
Query: 483 AYYFDWMAQEFNLSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPTAEDIGTPGQLAQ 542
Y + F L+ D + + D +IF L GA+L PT + G+ Q
Sbjct: 7162 VNYCVDAVRLFGLTPDDTVLQQNTLNFDLSVEEIFPALLAGATL-APTRQLFGSAE--LQ 7218
Query: 543 WANKYKVTVTHLTPAMGQLLAA-------QADEPIPSLHHAFFVGDILTKRDCLRLQ--- 592
+ T HLT A LAA QA + + + GD L+ + +LQ
Sbjct: 7219 QNGDIRPTFLHLTAAHWHTLAAEWHNSPAQARDYLRDVRLINVTGDALSTQ---KLQMWD 7275
Query: 593 -VLANNVNVVNMYGTTETQRSVSYFVVPARSQDQTFLESQKDVIPAGRGMKNVQLLVINR 651
V + +VN YG TE S + A + ++ GR M N ++ +++
Sbjct: 7276 AVRPAHTRLVNTYGPTEATVSCT-----AAYMEHDAVDGILGNASIGRPMANTRIYLLDA 7330
Query: 652 FDTNKICGIGEVGEIYLRAGGLAEGYLGNDELTSKKFLKSWFADPSKFVDRTPENAPWKP 711
+ G GEI++ G+A GYL D + +++FL F+D
Sbjct: 7331 H--RQPVPFGVAGEIFIGGDGVARGYLNLDAINAERFLADPFSDQPDA------------ 7376
Query: 712 YWFGIRDRMYRSGDLGRYLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRENI 771
R+Y++GDL RY P G +E GR D Q+K+RGFRIELGEI L V+E
Sbjct: 7377 -------RLYKTGDLARYRPDGRLEYLGRNDFQVKVRGFRIELGEIEARLGDCAGVKEAA 7429
Query: 772 TLVRRDKDEEPTLVAYIVPQ-GLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKTKL 830
+VR D + LVAY+VPQ G+N + +R L +L
Sbjct: 7430 VIVREDTPGDKRLVAYVVPQAGVNLN----------------------AASLRAQLAVQL 7467
Query: 831 PSYAIPSVIVPLHKMPLNPNGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERDIR 890
Y +P V L +PL PN K+D+ ALP PD A ASR+H + TE +
Sbjct: 7468 AEYMLPGAFVSLAALPLTPNRKLDRQALPAPDAE--AYASRTH-----EAPQGHTEIVLA 7520
Query: 891 DIWLRIIPHATDVNKKASFFDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGLAK 950
IW ++ V + FF++GGHS+L RLI ++R++ V + L VF++P + LA+
Sbjct: 7521 QIWQSLL-KVESVGRHDHFFELGGHSLLVMRLIAQVREQLGVELNLRDVFAQPQLSALAQ 7579
Query: 951 EIERMKSGEMISVMDIGKEE 970
+ + ++ + +E+
Sbjct: 7580 VLSQAAHSTRPGIVPVSREQ 7599
Score = 220 bits (561), Expect = 5e-60
Identities = 200/739 (27%), Positives = 320/739 (43%), Gaps = 114/739 (15%)
Query: 229 VNIVADQLLKLVVSASKDVTGPIGALDLMTPTQMN-VLPDPTVDLDWSGYRGAIQDIFAS 287
+NI + L++ + A + P+ +L ++ P + +L D + +F +
Sbjct: 2641 MNIALEHLVEALEQAPQT---PLHSLSILPPAERRQLLVDFNATTRSYPQDRTVHGLFEA 2697
Query: 288 NAAKFPDRECIVVTPSVTIDAPVTSYTYRQIDESSNILAHHLVKNGIERGDVVMVYAYRG 347
A + P+ +V S TY +++ +N L HL+ G++ GD V + R
Sbjct: 2698 RAKELPEATAVVHNGH--------SLTYAELNRRANCLTRHLIGLGVQPGDRVAILLERS 2749
Query: 348 VDLVVAVMGVLKAGATFSVIDPAYPPARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVE- 406
V L+V+ + +LK A + +D + P RQ + + R L+ L V P + V+
Sbjct: 2750 VGLLVSQLAILKCAAVYVPLDVSAPLERQDFMVRDSGARTLLTLSAMAV--PEGIARVDL 2807
Query: 407 KSLELKTYVPALKLAKDGSLTGGSVSKGADDILQHVLHLKSEQTGVVVGPDSTPTLSFTS 466
S+ L+T H L L + +S + +TS
Sbjct: 2808 DSVVLETEA------------------------DHDLRL-------IQSTESVAYIMYTS 2836
Query: 467 GSEGIPKGVKGRHFSL-AYYFDWMAQEFNLSESDRFTMLSGIAHDPIQRDIFTPLFLGAS 525
GS G PKGV H ++ + EFN DR S A D D++ PL G
Sbjct: 2837 GSTGTPKGVLVPHRAINRLVINNGYAEFNAQ--DRVAFASNPAFDASTLDVWAPLLNGGC 2894
Query: 526 LIVPTAEDIGTPGQLAQWANKYKVTVTHLTPAMGQLLAAQADEPIPSLHHAFFVGDILTK 585
++V + + T + A + V+V +T + AA L + GD+L
Sbjct: 2895 VVVVDQDVLLTQERFAALLQEQSVSVLWMTAGLFHQYAAGLMSVFAQLRYLIVGGDVLDP 2954
Query: 586 RDCLRLQVLANNVNVVNMYGTTETQRSVSYFVVPARSQDQTFLESQKDVIPAGRGMKNVQ 645
R+ +++N YG TE + + + + IP GR + N +
Sbjct: 2955 AVIGRVLKEGAPQHLLNGYGPTEATTFTTTHEIKSVGEGG---------IPIGRPIGNTR 3005
Query: 646 LLVINRFDTNKICGIGEVGEIYLRAGGLAEGYLGNDELTSKKFLKSWF-ADPSKFVDRTP 704
+ V++ + IG GE+Y+ G+A+GYL EL+++KF+ F ADP
Sbjct: 3006 VYVLDA--NRQPVPIGVAGELYIGGDGVAKGYLNRPELSAEKFVADPFNADPGAL----- 3058
Query: 705 ENAPWKPYWFGIRDRMYRSGDLGRYLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRH 764
+YR+GDL R+ G V+ GR DDQ+KIRGFRIELGEI L
Sbjct: 3059 ---------------LYRTGDLARWRADGAVDYLGRNDDQVKIRGFRIELGEIEARLGEC 3103
Query: 765 PNVRENITLVRRDKDEEPTLVAYIVPQGLNKDDFDSATESEDIVVNGLKKYRKLIHDIRE 824
P V++ + L R+D LV Y++P+ T S +H +RE
Sbjct: 3104 PGVKDAVVLARQDDAGPKRLVGYVIPE-------PGVTLS--------------VHALRE 3142
Query: 825 YLKTKLPSYAIPSVIVPLHKMPLNPNGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTA 884
L + L Y +PS V L PL NGK+D+ LP PD A ASR + + L
Sbjct: 3143 QLSSVLADYMLPSAFVVLPAFPLTANGKLDRRELPAPDAE--AYASREY-----EAPLGK 3195
Query: 885 TERDIRDIWLRIIPHATDVNKKASFFDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPT 944
E+ + IW ++ V + FF++GGHS+LA LI +R+ ++ + ++FS+PT
Sbjct: 3196 VEQTLAQIWADVL-KVEQVGRHDHFFELGGHSLLAVTLIERMRQA-GLSADVRVLFSQPT 3253
Query: 945 IEGLAKEIERMKSGEMISV 963
+ LA I SG +SV
Sbjct: 3254 LAALAAAI---GSGREVSV 3269
Score = 202 bits (513), Expect = 2e-54
Identities = 177/644 (27%), Positives = 279/644 (43%), Gaps = 106/644 (16%)
Query: 314 TYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPP 373
TYR++ E +N LAH L++ G++ G V + R +L+++ + +LK A + +D P
Sbjct: 9168 TYRELHERANHLAHCLLERGVQPGSRVAIMLNRSFELIISELAILKCAAVYVPLDHHAPE 9227
Query: 374 ARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKTYVPALKLAKDGSLTGGSVSK 433
RQ L + A VL T + L +P L L
Sbjct: 9228 ERQRFMLQ----------DSAAVLVLTT-----RDRALPEDMPRLDL------------- 9259
Query: 434 GADDILQHVLHLKSEQTGVVVGPDS-TPT-LSFTSGSEGIPKGVKGRHFSLAY------Y 485
D LQ + ++ + V G S TP + +TSGS G PKGV H ++ Y
Sbjct: 9260 ---DTLQLI---QAARPAVFPGQTSDTPAYIMYTSGSTGHPKGVIVPHLAIGRLAINNGY 9313
Query: 486 FDWMAQEFNLSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPTAEDIGTPGQLAQWAN 545
D+ Q DR S A D +++ L G +++ E + +P Q AQ
Sbjct: 9314 ADFGVQ-------DRVAFASNPAFDASTMEVWGALLNGGRVVIIDHETLLSPTQFAQALT 9366
Query: 546 KYKVTVTHLTPAMGQLLAAQADEPIPSLHHAFFVGDILTKRDCLRLQVLANNVNVVNMYG 605
+ +V+V +T AM + + L G+ RL A ++ +V+ YG
Sbjct: 9367 EAEVSVLFVTTAMFNQYLVLIPDALAGLRILLCGGERGDPASFRRLLAHAPSLRLVHCYG 9426
Query: 606 TTETQRSVSYFVVPARSQDQTFLESQKDVIPAGRGMKNVQLLVINRFDTNKICGIGEVGE 665
TET + VV A T +P GR + N ++ +++ + +G GE
Sbjct: 9427 PTETTTFATTHVVSAVPHGATS-------VPIGRPIANTRVYILDA--ARQPVPVGVAGE 9477
Query: 666 IYLRAGGLAEGYLGNDELTSKKFLKSWFADPSKFVDRTPENAPWKPYWFGIRDRMYRSGD 725
I++ G+A GYL +LT ++F+ F+D R+YRSGD
Sbjct: 9478 IHIGGVGVALGYLNRPDLTEERFIADPFSDSGSA-------------------RLYRSGD 9518
Query: 726 LGRYLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRENITLVRRDKDEEPTLV 785
LGR+LP G +E R D Q+KIRGFRIELGEI L VRE + + R D + L+
Sbjct: 9519 LGRWLPDGTIEYLDRNDGQVKIRGFRIELGEIEARLHECHGVREALVVAREDSPGDKCLI 9578
Query: 786 AYIVPQGLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKTKLPSYAIPSVIVPLHKM 845
AY + N D D+ +R L++ LP Y IP+ V L +
Sbjct: 9579 AYYT-EHENGGDLDADV-------------------LRAQLRSVLPEYMIPAAYVRLTAL 9618
Query: 846 PLNPNGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERDIRDIWLRIIPHATDVNK 905
PL NGK++ ALP P+ + + ++ TL D+ + + +
Sbjct: 9619 PLTLNGKVNTGALPVPEMHAYGSQDYEAPQGDLEVTLAGLWADVLKL--------EQIGR 9670
Query: 906 KASFFDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGLA 949
+FF++GGHS+LA RL+ L + + V L +F ++ +A
Sbjct: 9671 HDNFFELGGHSLLAIRLV-GLLAQANLTVSLAELFQHESVASMA 9713
Lambda K H
0.318 0.136 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 30,225
Number of extensions: 1368
Number of successful extensions: 65
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 14
Length of query: 1419
Length of database: 10,307
Length adjustment: 64
Effective length of query: 1355
Effective length of database: 10,243
Effective search space: 13879265
Effective search space used: 13879265
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 67 (30.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory