Align L-2-aminoadipate reductase; Alpha-aminoadipate reductase; Alpha-AR; L-aminoadipate-semialdehyde dehydrogenase; EC 1.2.1.31; EC 1.2.1.95 (characterized)
to candidate AO356_28220 AO356_28220 non-ribosomal peptide synthetase
Query= SwissProt::P40976
(1419 letters)
>FitnessBrowser__pseudo5_N2C3_1:AO356_28220
Length = 8596
Score = 256 bits (653), Expect = 9e-71
Identities = 215/740 (29%), Positives = 332/740 (44%), Gaps = 95/740 (12%)
Query: 215 LKLIYNQLLFSESRVNIVADQLLKLVVSASKDVTGPIGALDLMTPTQMNVLPDPTV-DLD 273
L IYN+ F + +AD+ + ++ +D T PI L T + ++L D
Sbjct: 401 LHWIYNESYFQADEIESMADRFMHVLEQGLRDDTLPIEGFVLPTAAEADMLQAWNASDAR 460
Query: 274 WSGYRGAIQDIFASNAAKFPDRECIVVTPSVTIDAPVTSYTYRQIDESSNILAHHLVKNG 333
+ I +F + PD +V + TY +++ +N +AH L+ G
Sbjct: 461 TYEHDHTIHGLFEAQVRARPD--------AVAVLHEDRCLTYGELNARANQVAHRLLILG 512
Query: 334 IERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPPARQIIYLSVAKPRALVVLED 393
+ D V + RG+D+++ ++G+LK+GA + +DPAYP R L + P AL+
Sbjct: 513 VHPDDRVAICVERGLDMIIGLLGILKSGAGYVPLDPAYPQERLAFMLDDSAPVALLTQST 572
Query: 394 AGVLSPTVVEYVEKSLELKTYVPALKLAKDGSLTGGSVSKGADDILQHVLHLKSEQTGVV 453
V P + VP L L + + + S+ D+ L S V
Sbjct: 573 LQVQLPAL------------QVPVLLLDQAEAAGITAQSRYNPDVRT----LASHHLAYV 616
Query: 454 VGPDSTPTLSFTSGSEGIPKGVKGRHFSLAYYFDWMAQEFNLSESDRFTMLSGIAHDPIQ 513
+ +TSGS G+PKGV H ++A F F D + + A D
Sbjct: 617 I---------YTSGSTGLPKGVMVEHRNVARLFSATQPWFEFGPQDVWALFHSFAFDFSV 667
Query: 514 RDIFTPLFLGASLIVPTAEDIGTPGQLAQWANKYKVTVTHLTP-AMGQLLAAQADEPI-P 571
+I+ L G L+V +P + VTV + TP A QL+AAQ D +
Sbjct: 668 WEIWGALTHGGRLLVVPQLVSRSPQDCYALLCEAGVTVLNQTPSAFRQLIAAQGDSDLCH 727
Query: 572 SLHHAFFVGDILTKRDCLRLQVLANNVN--VVNMYGTTETQRSVSYFVVPARSQDQTFLE 629
SL F G+ L A N +VNMYG TET V+Y + A T
Sbjct: 728 SLRQVIFGGEALETSMLKPWYARATNAGTQLVNMYGITETTVHVTYRALCAADAQLT--- 784
Query: 630 SQKDVIPAGRGMKNVQLLVINRFDTNKICGIGEVGEIYLRAGGLAEGYLGNDELTSKKFL 689
V P G+ + +++L +++++ + +G GE+Y+ G+A GYL +L +FL
Sbjct: 785 ---GVSPIGKRIPDLRLYLLDKY--GQPVPVGVEGELYVGGAGVARGYLNRPQLDETRFL 839
Query: 690 KSWFADPSKFVDRTPENAPWKPYWFGIRDRMYRSGDLGRYLPTGNVECSGRADDQIKIRG 749
P+ G RMYR+GDLGR+L G +E GR D+Q+KIRG
Sbjct: 840 AD-------------------PFDGGAHARMYRTGDLGRWLKNGELEYLGRNDEQVKIRG 880
Query: 750 FRIELGEINTHLSRHPNVRENITLVRRDKDEEPTLVAYIVPQGLNKDDFDSATESEDIVV 809
FRIELGEI L+ VRE + + R D + LVAY+V D+ +
Sbjct: 881 FRIELGEIEAKLAVCAQVREAVVIAREDNPGDKRLVAYVVA--------DAGRQLS---- 928
Query: 810 NGLKKYRKLIHDIREYLKTKLPSYAIPSVIVPLHKMPLNPNGKIDKPALPFPDTSQLAAA 869
+ D+R+ L L Y +PS V L +PL NGK+D+ ALP PD SQ A
Sbjct: 929 ---------VADLRDQLLGLLADYMVPSAFVLLDALPLTTNGKLDRKALPAPD-SQAYAR 978
Query: 870 SRSHSKHGVDETLTATERDIRDIWLRIIPHATDVNKKASFFDIGGHSILATRLIFELRKK 929
+ G ET+ A +W ++ V + FF++GGHS+LA +LI E ++
Sbjct: 979 RSYEAPEGEVETVLAR------LWAELL-GVEQVGRHDRFFELGGHSLLAVKLI-ERMRQ 1030
Query: 930 FAVNVPLGLVFSEPTIEGLA 949
++ +G++F +PT+ LA
Sbjct: 1031 VKLHADVGVLFGQPTLASLA 1050
Score = 247 bits (630), Expect = 4e-68
Identities = 203/726 (27%), Positives = 330/726 (45%), Gaps = 126/726 (17%)
Query: 281 IQDIFASNAAKFPDRECIVVTPSVTIDAPVTSYTYRQIDESSNILAHHLVKNGIERGDVV 340
+ +F A + PD +V + TY +++E +N LAH+L K G+E V
Sbjct: 1561 VHGLFEEQAQRTPD--------AVAVIRGEQRLTYHELNERANRLAHYLRKQGVEPDSRV 1612
Query: 341 MVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPPARQIIYLSVAKPRALVVLEDAGVLSPT 400
+ RG+D+VV ++ +LKAG + +DPAYP R L + P A VL+ T
Sbjct: 1613 AICVERGIDMVVGLLAILKAGGGYVPLDPAYPLDRIAYMLDDSAP--------AAVLAQT 1664
Query: 401 VVEYVEKSLELKTYVPALKLAKDGSLTGGSVSKG--ADDILQH--VLHLKSEQTGVVVGP 456
L+L + S+ ++ G D+ +Q+ V L S V+
Sbjct: 1665 AT---------------LELLAEASMPVINLDSGDWQDESVQNPEVTELTSSHLAYVI-- 1707
Query: 457 DSTPTLSFTSGSEGIPKGVKGRHFSLAYYFDWMAQEFNLSESDRFTMLSGIAHDPIQRDI 516
+TSGS G+PKGV H + + W + F+ + + + D +
Sbjct: 1708 -------YTSGSTGLPKGVMIEHRNTVNFLTWAHRSFDAQTLSKTLFSTSLNFDLAVYEC 1760
Query: 517 FTPLFLGASLIVPTAEDIGTPGQLAQWANKYKVTVTHLTPAMGQLLAAQAD--EPIPSLH 574
F PL G S+ V T L ++ +T+ + P+ + L E + +++
Sbjct: 1761 FAPLTSGGSIEVVT-------NVLELQQGEHDITLINTVPSALKALLESGGLGEGVDTVN 1813
Query: 575 HAFFVGDILTKRDCLRLQVLANNVNVVNMYGTTETQRSVSYFVVPARSQDQTFLESQKDV 634
A G+ L + L + N+YG +ET S+ + +++D
Sbjct: 1814 VA---GEALKRSLVETLFEQTQVKRLCNLYGPSETTTYSSWVSM-----------AREDG 1859
Query: 635 IPA--GRGMKNVQLLVINRFDTNKICGIGEVGEIYLRAGGLAEGYLGNDELTSKKFLKSW 692
A G+ + N Q +++ + + +G GEIY+ G+A GYL D+LT+++FLK
Sbjct: 1860 FAAHIGKPVANTQFYLLD--EHKQPVPLGVPGEIYIGGAGVARGYLNRDDLTAERFLKDP 1917
Query: 693 FADPSKFVDRTPENAPWKPYWFGIRDRMYRSGDLGRYLPTGNVECSGRADDQIKIRGFRI 752
F+ T NA RMY++GDLGRYLP GN+E GR DDQ+KIRGFRI
Sbjct: 1918 FS--------TTPNA-----------RMYKTGDLGRYLPDGNIEYLGRNDDQVKIRGFRI 1958
Query: 753 ELGEINTHLSRHPNVRENITLVRRDKDEEPTLVAYIVPQGLNKDDFDSATESEDIVVNGL 812
ELGEI L++ PN++E + L R D + LVAY F + E + + L
Sbjct: 1959 ELGEIEAKLAQAPNIKETVVLAREDVPGDKRLVAY----------FTQHSPDETVEIEAL 2008
Query: 813 KKYRKLIHDIREYLKTKLPSYAIPSVIVPLHKMPLNPNGKIDKPALPFPDTSQLAAASRS 872
R +L+ +LP+Y +P V L +PL PNGK+D+ ALP PD +
Sbjct: 2009 ----------RTHLQAQLPAYMVPVAYVRLDALPLTPNGKLDRKALPAPDLDAVIT---- 2054
Query: 873 HSKHGVDETLTATERDIRDIWLRIIPHATDVNKKASFFDIGGHSILATRLIFELRKKFAV 932
G + TE + IW ++ V + FF++GGHS+LA LI +R+ +
Sbjct: 2055 ---RGYEAPQGETETTLAQIWQDVL-KVERVGRHDHFFELGGHSLLAVSLIERMRQA-GL 2109
Query: 933 NVPLGLVFSEPTIEGLAKEIERMKSGEMISV----MDIGKEETREPEIEYGKDALDLVDL 988
+ + ++F++PT+ LA + +G ++V + +G E + K + + +D
Sbjct: 2110 SADVRILFNQPTLAALAAAV---GTGNEVTVPANLIPLGCEHITPAMLPLAKLSQEAIDR 2166
Query: 989 IPKEFP 994
I P
Sbjct: 2167 IVSTVP 2172
Score = 243 bits (619), Expect = 8e-67
Identities = 194/647 (29%), Positives = 299/647 (46%), Gaps = 111/647 (17%)
Query: 314 TYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPP 373
TY +++E +N LAH+L K G+E V + RG+D+VV ++ +LKAG + +DPAYP
Sbjct: 4806 TYHELNERANRLAHYLRKQGVEPDSRVAICVERGIDMVVGLLAILKAGGGYVPLDPAYPL 4865
Query: 374 ARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKTYVPALKLAKDGSLTGGSVSK 433
R L + P A VL+ T L+L + S+ ++
Sbjct: 4866 DRIAYMLDDSAP--------AAVLAQTAT---------------LELLAEASMPVINLDS 4902
Query: 434 G--ADDILQH--VLHLKSEQTGVVVGPDSTPTLSFTSGSEGIPKGVKGRHFSLAYYFDWM 489
G D+ +Q+ V L S V+ +TSGS G+PKGV H + + W
Sbjct: 4903 GDWQDESVQNPEVAELTSSHLAYVI---------YTSGSTGLPKGVMIEHRNTVNFLTWA 4953
Query: 490 AQEFNLSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPTAEDIGTPGQLAQWANKYKV 549
+ F+ + + + + D + F PL G S+ V T L ++ +
Sbjct: 4954 HRSFDDATLSKTLFSTSLNFDLAVYECFAPLTSGGSIEVVT-------NVLELQQGEHDI 5006
Query: 550 TVTHLTPAMGQLLAAQAD--EPIPSLHHAFFVGDILTKRDCLRLQVLANNVNVVNMYGTT 607
T+ + P+ + L E + +++ A G+ L + L + N+YG +
Sbjct: 5007 TLINTVPSALKALLESGGLGEGVDTVNVA---GEALKRSLVETLFEQTQVKRLCNLYGPS 5063
Query: 608 ETQRSVSYFVVPARSQDQTFLESQKDVIPA--GRGMKNVQLLVINRFDTNKICGIGEVGE 665
ET S+ + +++D A G+ + N Q +++ + + +G GE
Sbjct: 5064 ETTTYSSWVSM-----------AREDGFAAHIGKPVANTQFYLLD--EHKQPVPLGVPGE 5110
Query: 666 IYLRAGGLAEGYLGNDELTSKKFLKSWFADPSKFVDRTPENAPWKPYWFGIRDRMYRSGD 725
IY+ G+A GYL D+LT+++FLK F RT NA RMY++GD
Sbjct: 5111 IYIGGAGVARGYLNRDDLTAERFLKDPF--------RTAPNA-----------RMYKTGD 5151
Query: 726 LGRYLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRENITLVRRDKDEEPTLV 785
LGRYLP GN+E GR DDQ+KIRGFRIELGEI L++H + E + L R D + LV
Sbjct: 5152 LGRYLPDGNIEYLGRNDDQVKIRGFRIELGEIEAKLAQHAALNETVVLAREDVPGDKRLV 5211
Query: 786 AYIVPQGLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKTKLPSYAIPSVIVPLHKM 845
AY S ES D I +R YL+ LPSY +P V L +
Sbjct: 5212 AYFTQH--------SPDESVD------------IEALRIYLQALLPSYMVPVAYVRLDAL 5251
Query: 846 PLNPNGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERDIRDIWLRIIPHATDVNK 905
PL PNGK+D+ ALP PD L G + E + IW ++ V +
Sbjct: 5252 PLTPNGKLDRKALPAPDLDALIT-------RGYEAPQGEVEISLAQIWQDVL-KVERVGR 5303
Query: 906 KASFFDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGLAKEI 952
FF++GGHS+LA LI +R+ ++ + ++F +PT+ LA +
Sbjct: 5304 HDHFFELGGHSLLAVTLIERMRQA-GLSADVRVLFGQPTLAALAAAV 5349
Score = 242 bits (617), Expect = 1e-66
Identities = 197/645 (30%), Positives = 300/645 (46%), Gaps = 107/645 (16%)
Query: 314 TYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPP 373
TY +++E +N LAH+L K G+E V + RG+D+VV ++ +LKAG + +DPAYP
Sbjct: 2659 TYHELNERANRLAHYLRKQGVEPDSRVAICVERGIDMVVGLLAILKAGGGYVPLDPAYPL 2718
Query: 374 ARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKTYVPALKLAKDGSLTGGSVSK 433
R I Y+ L+D+ +P VV +LEL + D L
Sbjct: 2719 DR-IAYM----------LDDS---APAVVLAQTATLELLAAASMPVIDLDSGLW------ 2758
Query: 434 GADDILQH--VLHLKSEQTGVVVGPDSTPTLSFTSGSEGIPKGVKGRHFSLAYYFDWMAQ 491
D+ +Q+ V L S V+ +TSGS G+PKGV H + + W +
Sbjct: 2759 -QDESVQNPEVAELTSSHLAYVI---------YTSGSTGLPKGVMIEHRNTVNFLTWAHR 2808
Query: 492 EFNLSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPTAEDIGTPGQLAQWANKYKVTV 551
F+ + + + D + F PL G S+ V T L ++ +T+
Sbjct: 2809 SFDSQTLAKTLFSTSLNFDLAVYECFAPLTSGGSIEVVT-------NVLELQQGEHDITL 2861
Query: 552 THLTPAMGQLLAAQAD--EPIPSLHHAFFVGDILTKRDCLRLQVLANNVNVVNMYGTTET 609
+ P+ + L E + +++ A G+ L + L + N+YG +ET
Sbjct: 2862 INTVPSALKALLESGGLGEGVDTVNVA---GEALKRSLVETLFEQTQVKRLCNLYGPSET 2918
Query: 610 QRSVSYFVVPARSQDQTFLESQKDVIPA--GRGMKNVQLLVINRFDTNKICGIGEVGEIY 667
S+ + +++D A G+ + N Q +++ + + +G GEIY
Sbjct: 2919 TTYSSWVSM-----------AREDGFAAHIGKPVANTQFYLLD--EHKQPVPLGVPGEIY 2965
Query: 668 LRAGGLAEGYLGNDELTSKKFLKSWFADPSKFVDRTPENAPWKPYWFGIRDRMYRSGDLG 727
+ G+A GYL D+LT+++FLK DP V RMY++GDLG
Sbjct: 2966 IGGAGVARGYLNRDDLTAERFLK----DPFSAVPNA---------------RMYKTGDLG 3006
Query: 728 RYLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRENITLVRRDKDEEPTLVAY 787
RYLP GN+E GR DDQ+KIRGFRIELGEI L++H + E + L R D + LVAY
Sbjct: 3007 RYLPDGNIEYLGRNDDQVKIRGFRIELGEIEAKLAQHAALNETVVLAREDVPGDKRLVAY 3066
Query: 788 IVPQGLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKTKLPSYAIPSVIVPLHKMPL 847
S ES D I +R YL+ LPSY +P V L +PL
Sbjct: 3067 FTQH--------SPDESVD------------IEALRAYLQALLPSYMVPVAYVRLDALPL 3106
Query: 848 NPNGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERDIRDIWLRIIPHATDVNKKA 907
PNGK+D+ ALP PD A SR G + TE + IW ++ V +
Sbjct: 3107 TPNGKLDRKALPAPDLD--AVISR-----GYEAPQGETETTLAQIWQDLL-GLQQVGRHD 3158
Query: 908 SFFDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGLAKEI 952
FF++GGHS+LA LI +R+ ++ + ++F +PT+ LA +
Sbjct: 3159 HFFELGGHSLLAVTLIERMRQA-GLSADVRILFGQPTLAALAAAV 3202
Score = 242 bits (617), Expect = 1e-66
Identities = 199/661 (30%), Positives = 309/661 (46%), Gaps = 118/661 (17%)
Query: 314 TYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPP 373
TY +++ +N +AH L+ G+ D V + R +++VV ++GVLKAGA + +DPAYP
Sbjct: 3732 TYCELNARANQVAHRLLALGVCPDDRVAICVERSLEMVVGLLGVLKAGAGYVPVDPAYP- 3790
Query: 374 ARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKTYVPALKLAKDGSLTGGSVSK 433
A +I YL L+D+ ++ +V+ V + L VP + L G SVS
Sbjct: 3791 AERIAYL----------LQDSAPVA-VLVQAVTQGLLAAGAVPVINLDNAG-WQDESVSN 3838
Query: 434 GADDILQHVLHLKSEQTGVVVGPDSTPTLSFTSGSEGIPKGVKGRHFSLAYYFDWMAQEF 493
A V L++ V+ +TSGS G+PKGV H +L+ W Q F
Sbjct: 3839 PA------VPGLEARHLAYVI---------YTSGSTGLPKGVMVEHRNLSNLVGWHCQAF 3883
Query: 494 NLSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPTA----EDIGTPGQLAQWANKYKV 549
N+ R + ++G D +I+ L GA+L++P A ED+G L W +
Sbjct: 3884 NVKRGSRTSSVAGFGFDAAAWEIWPSLCAGATLLLPPAHAGSEDVGA---LLDWWQAQAL 3940
Query: 550 TVTHLTPAMGQLLAAQADEPIPSLHHAFF-------VGDILTKRDCLRLQVLANNVNVVN 602
V L P P +AF + +L D LR ++N
Sbjct: 3941 DVCFL--------------PTPIAEYAFGRNLGHDQLRTLLIGGDRLRKLPADLPFELIN 3986
Query: 603 MYGTTETQRSVSYFVVPARSQDQTFLESQKDVIPAGRGMKNVQLLVINRFDTNKICGIGE 662
YG TET VV + +++ + V+ G+ + N Q+ +++ + +G
Sbjct: 3987 NYGPTETT------VVATSGR----IDASQAVLHIGKPVANTQVYLLDAH--LQPVPVGV 4034
Query: 663 VGEIYLRAGGLAEGYLGNDELTSKKFLKSWFADPSKFVDRTPENAPWKPYWFGIRDRMYR 722
GE+Y+ G+A GYL +LT+++F+K DP V RMYR
Sbjct: 4035 AGELYIGGAGVARGYLNRGQLTAERFVK----DPFSLVQDA---------------RMYR 4075
Query: 723 SGDLGRYLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRENITLVRRDKDEEP 782
+GDLGRYLP GN++ GR D Q+KIRG RIELGEI L VRE + + + ++
Sbjct: 4076 TGDLGRYLPDGNIDYLGRNDSQLKIRGLRIELGEIEARLGACAGVREAVVVAVGEAPDDQ 4135
Query: 783 TLVAYIVPQGLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKTKLPSYAIPSVIVPL 842
LVAY D D A ++ +RE L+ LPS+ +P+ + L
Sbjct: 4136 RLVAYYTAH----DTLDQALTAD---------------SLREQLQVHLPSHMVPAAYMCL 4176
Query: 843 HKMPLNPNGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERDIRDIWLRIIPHATD 902
+PL PNGK+D ALP PD S ++S G E + IW ++
Sbjct: 4177 DALPLTPNGKLDHRALPVPD-------SEAYSGRGYAAPQGEVETALARIWSELL-KVEQ 4228
Query: 903 VNKKASFFDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGLAKEIERMKSGEMIS 962
V + +FF++GGHS++A LI E ++ ++ + ++FS+PT+ LA + SG +S
Sbjct: 4229 VGRYDNFFELGGHSLMAVSLI-ERMRQVGLSADVRVLFSQPTLAALAAAV---GSGSEVS 4284
Query: 963 V 963
V
Sbjct: 4285 V 4285
Score = 237 bits (604), Expect = 4e-65
Identities = 191/644 (29%), Positives = 297/644 (46%), Gaps = 91/644 (14%)
Query: 314 TYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPP 373
TY +++ +N LAH L+ GI D V + RG+D++V ++G+LK+GA + +DPA P
Sbjct: 8031 TYGELNAQANQLAHRLLSLGIRPDDRVAICVERGLDMLVGLLGILKSGAGYVPLDPASPA 8090
Query: 374 ARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKTYVPALKLAKDGSLTGGSVSK 433
R L + P A+VV L + E + +EL + PAL+ +
Sbjct: 8091 ERIAYMLEDSAPVAIVVHAATQAL---LAEESVRLIELDS--PALRSQSTAN-------- 8137
Query: 434 GADDILQHVLHLKSEQTGVVVGPDSTPTLSFTSGSEGIPKGVKGRHFSLAYYFDWMAQEF 493
V S Q V+ +TSGS G+PKGV H ++A F F
Sbjct: 8138 ------PQVPGQTSSQLAYVI---------YTSGSTGLPKGVMVEHRNVARLFSATQPWF 8182
Query: 494 NLSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPTAEDIGTPGQLAQWANKYKVTVTH 553
+ D + + A D +I+ L G L+V +P + + VTV +
Sbjct: 8183 EFGQQDVWALFHSFAFDFSVWEIWGALIHGGRLLVVPQLVSRSPQECYALLCQAGVTVLN 8242
Query: 554 LTP-AMGQLLAAQADEPIP-SLHHAFFVGDILTKRDCLR---LQVLANNVNVVNMYGTTE 608
TP A QL+ AQ + + SL F G+ L + L+ +V +VNMYG TE
Sbjct: 8243 QTPSAFRQLIVAQGESDLRHSLRQVIFGGEAL-ETAMLKPWYARVANAGTQLVNMYGITE 8301
Query: 609 TQRSVSYFVVPARSQDQTFLESQKDVIPAGRGMKNVQLLVINRFDTNKICGIGEVGEIYL 668
T V+Y + A T V P G+ + ++QL V++ + +G VGE+Y+
Sbjct: 8302 TTVHVTYRPLEAADAQLT------GVSPIGKRIPDLQLYVLDA--RREPVPVGVVGEMYV 8353
Query: 669 RAGGLAEGYLGNDELTSKKFLKSWFADPSKFVDRTPENAPWKPYWFGIRDRMYRSGDLGR 728
G+A GYL ELT ++F+ F+ G R+YR+GDLGR
Sbjct: 8354 GGAGVARGYLNRPELTQERFIADTFSG-------------------GEGARLYRTGDLGR 8394
Query: 729 YLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRENITLVRRDKDEEPTLVAYI 788
+L G++E GR DDQ+KIRGFRIELGEI L+ V + + + R D + LV Y+
Sbjct: 8395 WLADGSIEYLGRNDDQVKIRGFRIELGEIEATLAACEGVSDALVIAREDAPGDKRLVGYV 8454
Query: 789 VPQGLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKTKLPSYAIPSVIVPLHKMPLN 848
+ D A + L ++R L L Y +PS V L PL
Sbjct: 8455 IAA-------DGA--------------QLLAAELRAQLLASLADYMVPSAFVVLEAFPLT 8493
Query: 849 PNGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERDIRDIWLRIIPHATDVNKKAS 908
NGK+D+ ALP PD S +A + + + A +D+ D+ + + +
Sbjct: 8494 TNGKLDRKALPAPDQSAVATREYEAPQGETETVIAAIWQDLLDL--------ERIGRHDN 8545
Query: 909 FFDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGLAKEI 952
FF++GGHS+LA +L+ E + ++ + ++F +PT+ LA +
Sbjct: 8546 FFELGGHSLLAVKLL-ERMRHVGLSADVRVLFGQPTLAALAAAV 8588
Score = 228 bits (580), Expect = 3e-62
Identities = 185/645 (28%), Positives = 295/645 (45%), Gaps = 104/645 (16%)
Query: 314 TYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPP 373
+Y Q++E +N LAHHL+ G++ D V + R ++L+ + + +LK A + +D
Sbjct: 6956 SYAQLNEQANRLAHHLIGLGVQPDDCVAILLPRSIELLASQLAILKCAAAYVPLDRNASL 7015
Query: 374 ARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKTYVPALKLAKDGSLTGGSVSK 433
RQ L + + L+ + TV E + ++L T L G + ++++
Sbjct: 7016 ERQGFMLDDCQAKCLLTFS-----TETVPEGASR-IDLDT------LDSQGPVHNPALAQ 7063
Query: 434 GADDILQHVLHLKSEQTGVVVGPDSTPTLSFTSGSEGIPKGVKGRHFSLAY------YFD 487
++ I + +TSGS G PKGV H ++ Y D
Sbjct: 7064 SSESIAY---------------------IMYTSGSTGQPKGVLVPHRAINRLVINNGYAD 7102
Query: 488 WMAQEFNLSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPTAEDIGTPGQLAQWANKY 547
+ AQ DR S A D D++ L G ++V E + P + AQ
Sbjct: 7103 FNAQ-------DRIAFASNPAFDASTMDVWGALLNGGQVVVIDHETLLEPSRFAQVLQDS 7155
Query: 548 KVTVTHLTPAMGQLLAAQADEPIPSLHHAFFVGDILTKRDCLRLQVLANNVNVVNMYGTT 607
VTV +T A+ + + L G+ RL LA + +V+ YG T
Sbjct: 7156 GVTVLFVTTAIFNQYVQLIPQALGGLRILLCGGERADVASFRRLLDLAPGLRLVHCYGPT 7215
Query: 608 ETQRSVSYFVVPARSQDQTFLESQKDVIPAGRGMKNVQLLVINRFDTNKICGIGEVGEIY 667
ET + V A + D + +P G + N Q+ V++ ++ +G VGE+Y
Sbjct: 7216 ETTTYATTLEVKAVALD-------AECVPIGGPIGNTQVYVLDA--RQQLAPLGVVGEMY 7266
Query: 668 LRAGGLAEGYLGNDELTSKKFLKSWFADPSKFVDRTPENAPWKPYWFGIRDRMYRSGDLG 727
+ G+A+GYL +LT++KF+ ADP P+ +YR+GDLG
Sbjct: 7267 IGGQGVAKGYLNRPDLTAEKFI----ADP---FSHEPDAL------------LYRTGDLG 7307
Query: 728 RYLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRENITLVRRDKDEEPTLVAY 787
R+LP G++EC GR DDQ+KIRGFRIELGEI L VR+ + LVR D+ E LVAY
Sbjct: 7308 RWLPEGSLECLGRNDDQVKIRGFRIELGEIEAKLVACDGVRDAVVLVRADETGEKRLVAY 7367
Query: 788 IVPQGLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKTKLPSYAIPSVIVPLHKMPL 847
++ Q + V GL RE L + L Y +P+ V L PL
Sbjct: 7368 VIAQ-----------PQVTLSVAGL----------REQLSSTLSEYMVPAAFVMLPAFPL 7406
Query: 848 NPNGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERDIRDIWLRIIPHATDVNKKA 907
NGK+D+ ALP PD A+ + + + +++ L +W ++ + +
Sbjct: 7407 TLNGKVDRKALPAPDAEAYASQAYAAPQGEIEQVLAG-------MWAELL-KVERIGRHD 7458
Query: 908 SFFDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGLAKEI 952
FF++GGHS+LA LI +R+ ++ + ++FS+PT+ LA I
Sbjct: 7459 HFFELGGHSLLAVTLIERMRQA-GLSADVRVLFSQPTLAALAAAI 7502
Score = 226 bits (576), Expect = 8e-62
Identities = 191/654 (29%), Positives = 297/654 (45%), Gaps = 100/654 (15%)
Query: 312 SYTYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAY 371
S TY +++ +N LA HLV G+ GD V + R ++L+V+ + +LK A + +D +
Sbjct: 5877 SLTYAELNHRANRLARHLVGLGVRPGDRVAIALERSLELLVSQLAILKCAAVYVPLDVSA 5936
Query: 372 PPARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKTYVPALKLAKDGSLTGGSV 431
P RQ V A VVL ++P + V+ L T V
Sbjct: 5937 PLERQ--QFMVQDSGAQVVLTSGTAVAPEASQRVD----LDTLV---------------F 5975
Query: 432 SKGADDILQHVLHLKSEQTGVVVGPDSTPTLSFTSGSEGIPKGVKGRHFSL-AYYFDWMA 490
++ AD+ LHL Q+G +S + +TSGS G PKGV H ++ +
Sbjct: 5976 NEAADN-----LHLT--QSG-----ESVAYIMYTSGSTGTPKGVLVPHRAINRLVINNGY 6023
Query: 491 QEFNLSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPTAEDIGTPGQLAQWANKYKVT 550
EFN DR S A D D++ PL G ++V + + T + A + V+
Sbjct: 6024 AEFNAQ--DRVAFASNPAFDASTLDVWAPLLNGGCVVVVDQDVLLTQERFAALLQEQSVS 6081
Query: 551 VTHLTPAMGQLLAAQADEPIPSLHHAFFVGDILTKRDCLRLQVLANNVNVVNMYGTTETQ 610
V +T + AA L + GD+L R+ ++++N YG TE
Sbjct: 6082 VLWMTAGLFHQYAAGLMSVFAQLRYLIVGGDVLDPAVIGRVLKEGAPLHLLNGYGPTEAT 6141
Query: 611 RSVSYFVVPARSQDQTFLESQKDVIPAGRGMKNVQLLVINRFDTNKICGIGEVGEIYLRA 670
+ + + + IP GR + N ++ V++ + IG GE+Y+
Sbjct: 6142 TFTTTHEIKSVGEGG---------IPIGRPIGNTRVYVLDA--NQQPVPIGVAGELYIGG 6190
Query: 671 GGLAEGYLGNDELTSKKFLKSWF-ADPSKFVDRTPENAPWKPYWFGIRDRMYRSGDLGRY 729
G+A+GYL EL+++KF+ F ADP +YR+GDL R+
Sbjct: 6191 DGVAKGYLNRPELSAEKFVADPFNADPGAL--------------------LYRTGDLARW 6230
Query: 730 LPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRENITLVRRDKDEEPTLVAYIV 789
G V+ GR DDQ+KIRGFRIELGEI L +H V++ + LVR D E LVAY
Sbjct: 6231 RADGTVDYLGRNDDQVKIRGFRIELGEIEARLGQHDEVKDVVVLVREDVPGEKRLVAYFT 6290
Query: 790 PQGLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKTKLPSYAIPSVIVPLHKMPLNP 849
P+ D D A I +R +L+ +LP Y IP + L +PL
Sbjct: 6291 PR-----DLDVAPH---------------IETLRTHLQGQLPDYMIPVAYIRLDTLPLTA 6330
Query: 850 NGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERDIRDIWLRIIPHATDVNKKASF 909
NGK+D+ ALP PD+ + + V++ L +W ++ V + +F
Sbjct: 6331 NGKLDRRALPAPDSEAYVSREYEAPQGEVEQLLA-------QLWAELL-RVEQVGRHDNF 6382
Query: 910 FDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGLAKEIERMKSGEMISV 963
F++GGHS+LA LI E ++ ++ + ++F +PT+ LA I SG +SV
Sbjct: 6383 FELGGHSLLAVTLI-ERMRQVGLSADVRVLFGQPTLAALAAAI---GSGREVSV 6432
Lambda K H
0.318 0.136 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 25,409
Number of extensions: 1183
Number of successful extensions: 60
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 8
Length of query: 1419
Length of database: 8596
Length adjustment: 63
Effective length of query: 1356
Effective length of database: 8533
Effective search space: 11570748
Effective search space used: 11570748
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 67 (30.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory