Align L-2-aminoadipate reductase; Alpha-aminoadipate reductase; Alpha-AR; L-aminoadipate-semialdehyde dehydrogenase; EC 1.2.1.31; EC 1.2.1.95 (characterized)
to candidate AO356_28220 AO356_28220 non-ribosomal peptide synthetase
Query= SwissProt::P40976
(1419 letters)
>FitnessBrowser__pseudo5_N2C3_1:AO356_28220
Length = 8596
Score = 256 bits (653), Expect = 9e-71
Identities = 215/740 (29%), Positives = 332/740 (44%), Gaps = 95/740 (12%)
Query: 215 LKLIYNQLLFSESRVNIVADQLLKLVVSASKDVTGPIGALDLMTPTQMNVLPDPTV-DLD 273
L IYN+ F + +AD+ + ++ +D T PI L T + ++L D
Sbjct: 401 LHWIYNESYFQADEIESMADRFMHVLEQGLRDDTLPIEGFVLPTAAEADMLQAWNASDAR 460
Query: 274 WSGYRGAIQDIFASNAAKFPDRECIVVTPSVTIDAPVTSYTYRQIDESSNILAHHLVKNG 333
+ I +F + PD +V + TY +++ +N +AH L+ G
Sbjct: 461 TYEHDHTIHGLFEAQVRARPD--------AVAVLHEDRCLTYGELNARANQVAHRLLILG 512
Query: 334 IERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPPARQIIYLSVAKPRALVVLED 393
+ D V + RG+D+++ ++G+LK+GA + +DPAYP R L + P AL+
Sbjct: 513 VHPDDRVAICVERGLDMIIGLLGILKSGAGYVPLDPAYPQERLAFMLDDSAPVALLTQST 572
Query: 394 AGVLSPTVVEYVEKSLELKTYVPALKLAKDGSLTGGSVSKGADDILQHVLHLKSEQTGVV 453
V P + VP L L + + + S+ D+ L S V
Sbjct: 573 LQVQLPAL------------QVPVLLLDQAEAAGITAQSRYNPDVRT----LASHHLAYV 616
Query: 454 VGPDSTPTLSFTSGSEGIPKGVKGRHFSLAYYFDWMAQEFNLSESDRFTMLSGIAHDPIQ 513
+ +TSGS G+PKGV H ++A F F D + + A D
Sbjct: 617 I---------YTSGSTGLPKGVMVEHRNVARLFSATQPWFEFGPQDVWALFHSFAFDFSV 667
Query: 514 RDIFTPLFLGASLIVPTAEDIGTPGQLAQWANKYKVTVTHLTP-AMGQLLAAQADEPI-P 571
+I+ L G L+V +P + VTV + TP A QL+AAQ D +
Sbjct: 668 WEIWGALTHGGRLLVVPQLVSRSPQDCYALLCEAGVTVLNQTPSAFRQLIAAQGDSDLCH 727
Query: 572 SLHHAFFVGDILTKRDCLRLQVLANNVN--VVNMYGTTETQRSVSYFVVPARSQDQTFLE 629
SL F G+ L A N +VNMYG TET V+Y + A T
Sbjct: 728 SLRQVIFGGEALETSMLKPWYARATNAGTQLVNMYGITETTVHVTYRALCAADAQLT--- 784
Query: 630 SQKDVIPAGRGMKNVQLLVINRFDTNKICGIGEVGEIYLRAGGLAEGYLGNDELTSKKFL 689
V P G+ + +++L +++++ + +G GE+Y+ G+A GYL +L +FL
Sbjct: 785 ---GVSPIGKRIPDLRLYLLDKY--GQPVPVGVEGELYVGGAGVARGYLNRPQLDETRFL 839
Query: 690 KSWFADPSKFVDRTPENAPWKPYWFGIRDRMYRSGDLGRYLPTGNVECSGRADDQIKIRG 749
P+ G RMYR+GDLGR+L G +E GR D+Q+KIRG
Sbjct: 840 AD-------------------PFDGGAHARMYRTGDLGRWLKNGELEYLGRNDEQVKIRG 880
Query: 750 FRIELGEINTHLSRHPNVRENITLVRRDKDEEPTLVAYIVPQGLNKDDFDSATESEDIVV 809
FRIELGEI L+ VRE + + R D + LVAY+V D+ +
Sbjct: 881 FRIELGEIEAKLAVCAQVREAVVIAREDNPGDKRLVAYVVA--------DAGRQLS---- 928
Query: 810 NGLKKYRKLIHDIREYLKTKLPSYAIPSVIVPLHKMPLNPNGKIDKPALPFPDTSQLAAA 869
+ D+R+ L L Y +PS V L +PL NGK+D+ ALP PD SQ A
Sbjct: 929 ---------VADLRDQLLGLLADYMVPSAFVLLDALPLTTNGKLDRKALPAPD-SQAYAR 978
Query: 870 SRSHSKHGVDETLTATERDIRDIWLRIIPHATDVNKKASFFDIGGHSILATRLIFELRKK 929
+ G ET+ A +W ++ V + FF++GGHS+LA +LI E ++
Sbjct: 979 RSYEAPEGEVETVLAR------LWAELL-GVEQVGRHDRFFELGGHSLLAVKLI-ERMRQ 1030
Query: 930 FAVNVPLGLVFSEPTIEGLA 949
++ +G++F +PT+ LA
Sbjct: 1031 VKLHADVGVLFGQPTLASLA 1050
Score = 247 bits (630), Expect = 4e-68
Identities = 203/726 (27%), Positives = 330/726 (45%), Gaps = 126/726 (17%)
Query: 281 IQDIFASNAAKFPDRECIVVTPSVTIDAPVTSYTYRQIDESSNILAHHLVKNGIERGDVV 340
+ +F A + PD +V + TY +++E +N LAH+L K G+E V
Sbjct: 1561 VHGLFEEQAQRTPD--------AVAVIRGEQRLTYHELNERANRLAHYLRKQGVEPDSRV 1612
Query: 341 MVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPPARQIIYLSVAKPRALVVLEDAGVLSPT 400
+ RG+D+VV ++ +LKAG + +DPAYP R L + P A VL+ T
Sbjct: 1613 AICVERGIDMVVGLLAILKAGGGYVPLDPAYPLDRIAYMLDDSAP--------AAVLAQT 1664
Query: 401 VVEYVEKSLELKTYVPALKLAKDGSLTGGSVSKG--ADDILQH--VLHLKSEQTGVVVGP 456
L+L + S+ ++ G D+ +Q+ V L S V+
Sbjct: 1665 AT---------------LELLAEASMPVINLDSGDWQDESVQNPEVTELTSSHLAYVI-- 1707
Query: 457 DSTPTLSFTSGSEGIPKGVKGRHFSLAYYFDWMAQEFNLSESDRFTMLSGIAHDPIQRDI 516
+TSGS G+PKGV H + + W + F+ + + + D +
Sbjct: 1708 -------YTSGSTGLPKGVMIEHRNTVNFLTWAHRSFDAQTLSKTLFSTSLNFDLAVYEC 1760
Query: 517 FTPLFLGASLIVPTAEDIGTPGQLAQWANKYKVTVTHLTPAMGQLLAAQAD--EPIPSLH 574
F PL G S+ V T L ++ +T+ + P+ + L E + +++
Sbjct: 1761 FAPLTSGGSIEVVT-------NVLELQQGEHDITLINTVPSALKALLESGGLGEGVDTVN 1813
Query: 575 HAFFVGDILTKRDCLRLQVLANNVNVVNMYGTTETQRSVSYFVVPARSQDQTFLESQKDV 634
A G+ L + L + N+YG +ET S+ + +++D
Sbjct: 1814 VA---GEALKRSLVETLFEQTQVKRLCNLYGPSETTTYSSWVSM-----------AREDG 1859
Query: 635 IPA--GRGMKNVQLLVINRFDTNKICGIGEVGEIYLRAGGLAEGYLGNDELTSKKFLKSW 692
A G+ + N Q +++ + + +G GEIY+ G+A GYL D+LT+++FLK
Sbjct: 1860 FAAHIGKPVANTQFYLLD--EHKQPVPLGVPGEIYIGGAGVARGYLNRDDLTAERFLKDP 1917
Query: 693 FADPSKFVDRTPENAPWKPYWFGIRDRMYRSGDLGRYLPTGNVECSGRADDQIKIRGFRI 752
F+ T NA RMY++GDLGRYLP GN+E GR DDQ+KIRGFRI
Sbjct: 1918 FS--------TTPNA-----------RMYKTGDLGRYLPDGNIEYLGRNDDQVKIRGFRI 1958
Query: 753 ELGEINTHLSRHPNVRENITLVRRDKDEEPTLVAYIVPQGLNKDDFDSATESEDIVVNGL 812
ELGEI L++ PN++E + L R D + LVAY F + E + + L
Sbjct: 1959 ELGEIEAKLAQAPNIKETVVLAREDVPGDKRLVAY----------FTQHSPDETVEIEAL 2008
Query: 813 KKYRKLIHDIREYLKTKLPSYAIPSVIVPLHKMPLNPNGKIDKPALPFPDTSQLAAASRS 872
R +L+ +LP+Y +P V L +PL PNGK+D+ ALP PD +
Sbjct: 2009 ----------RTHLQAQLPAYMVPVAYVRLDALPLTPNGKLDRKALPAPDLDAVIT---- 2054
Query: 873 HSKHGVDETLTATERDIRDIWLRIIPHATDVNKKASFFDIGGHSILATRLIFELRKKFAV 932
G + TE + IW ++ V + FF++GGHS+LA LI +R+ +
Sbjct: 2055 ---RGYEAPQGETETTLAQIWQDVL-KVERVGRHDHFFELGGHSLLAVSLIERMRQA-GL 2109
Query: 933 NVPLGLVFSEPTIEGLAKEIERMKSGEMISV----MDIGKEETREPEIEYGKDALDLVDL 988
+ + ++F++PT+ LA + +G ++V + +G E + K + + +D
Sbjct: 2110 SADVRILFNQPTLAALAAAV---GTGNEVTVPANLIPLGCEHITPAMLPLAKLSQEAIDR 2166
Query: 989 IPKEFP 994
I P
Sbjct: 2167 IVSTVP 2172
Score = 243 bits (619), Expect = 8e-67
Identities = 194/647 (29%), Positives = 299/647 (46%), Gaps = 111/647 (17%)
Query: 314 TYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPP 373
TY +++E +N LAH+L K G+E V + RG+D+VV ++ +LKAG + +DPAYP
Sbjct: 4806 TYHELNERANRLAHYLRKQGVEPDSRVAICVERGIDMVVGLLAILKAGGGYVPLDPAYPL 4865
Query: 374 ARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKTYVPALKLAKDGSLTGGSVSK 433
R L + P A VL+ T L+L + S+ ++
Sbjct: 4866 DRIAYMLDDSAP--------AAVLAQTAT---------------LELLAEASMPVINLDS 4902
Query: 434 G--ADDILQH--VLHLKSEQTGVVVGPDSTPTLSFTSGSEGIPKGVKGRHFSLAYYFDWM 489
G D+ +Q+ V L S V+ +TSGS G+PKGV H + + W
Sbjct: 4903 GDWQDESVQNPEVAELTSSHLAYVI---------YTSGSTGLPKGVMIEHRNTVNFLTWA 4953
Query: 490 AQEFNLSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPTAEDIGTPGQLAQWANKYKV 549
+ F+ + + + + D + F PL G S+ V T L ++ +
Sbjct: 4954 HRSFDDATLSKTLFSTSLNFDLAVYECFAPLTSGGSIEVVT-------NVLELQQGEHDI 5006
Query: 550 TVTHLTPAMGQLLAAQAD--EPIPSLHHAFFVGDILTKRDCLRLQVLANNVNVVNMYGTT 607
T+ + P+ + L E + +++ A G+ L + L + N+YG +
Sbjct: 5007 TLINTVPSALKALLESGGLGEGVDTVNVA---GEALKRSLVETLFEQTQVKRLCNLYGPS 5063
Query: 608 ETQRSVSYFVVPARSQDQTFLESQKDVIPA--GRGMKNVQLLVINRFDTNKICGIGEVGE 665
ET S+ + +++D A G+ + N Q +++ + + +G GE
Sbjct: 5064 ETTTYSSWVSM-----------AREDGFAAHIGKPVANTQFYLLD--EHKQPVPLGVPGE 5110
Query: 666 IYLRAGGLAEGYLGNDELTSKKFLKSWFADPSKFVDRTPENAPWKPYWFGIRDRMYRSGD 725
IY+ G+A GYL D+LT+++FLK F RT NA RMY++GD
Sbjct: 5111 IYIGGAGVARGYLNRDDLTAERFLKDPF--------RTAPNA-----------RMYKTGD 5151
Query: 726 LGRYLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRENITLVRRDKDEEPTLV 785
LGRYLP GN+E GR DDQ+KIRGFRIELGEI L++H + E + L R D + LV
Sbjct: 5152 LGRYLPDGNIEYLGRNDDQVKIRGFRIELGEIEAKLAQHAALNETVVLAREDVPGDKRLV 5211
Query: 786 AYIVPQGLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKTKLPSYAIPSVIVPLHKM 845
AY S ES D I +R YL+ LPSY +P V L +
Sbjct: 5212 AYFTQH--------SPDESVD------------IEALRIYLQALLPSYMVPVAYVRLDAL 5251
Query: 846 PLNPNGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERDIRDIWLRIIPHATDVNK 905
PL PNGK+D+ ALP PD L G + E + IW ++ V +
Sbjct: 5252 PLTPNGKLDRKALPAPDLDALIT-------RGYEAPQGEVEISLAQIWQDVL-KVERVGR 5303
Query: 906 KASFFDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGLAKEI 952
FF++GGHS+LA LI +R+ ++ + ++F +PT+ LA +
Sbjct: 5304 HDHFFELGGHSLLAVTLIERMRQA-GLSADVRVLFGQPTLAALAAAV 5349
Score = 242 bits (617), Expect = 1e-66
Identities = 197/645 (30%), Positives = 300/645 (46%), Gaps = 107/645 (16%)
Query: 314 TYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPP 373
TY +++E +N LAH+L K G+E V + RG+D+VV ++ +LKAG + +DPAYP
Sbjct: 2659 TYHELNERANRLAHYLRKQGVEPDSRVAICVERGIDMVVGLLAILKAGGGYVPLDPAYPL 2718
Query: 374 ARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKTYVPALKLAKDGSLTGGSVSK 433
R I Y+ L+D+ +P VV +LEL + D L
Sbjct: 2719 DR-IAYM----------LDDS---APAVVLAQTATLELLAAASMPVIDLDSGLW------ 2758
Query: 434 GADDILQH--VLHLKSEQTGVVVGPDSTPTLSFTSGSEGIPKGVKGRHFSLAYYFDWMAQ 491
D+ +Q+ V L S V+ +TSGS G+PKGV H + + W +
Sbjct: 2759 -QDESVQNPEVAELTSSHLAYVI---------YTSGSTGLPKGVMIEHRNTVNFLTWAHR 2808
Query: 492 EFNLSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPTAEDIGTPGQLAQWANKYKVTV 551
F+ + + + D + F PL G S+ V T L ++ +T+
Sbjct: 2809 SFDSQTLAKTLFSTSLNFDLAVYECFAPLTSGGSIEVVT-------NVLELQQGEHDITL 2861
Query: 552 THLTPAMGQLLAAQAD--EPIPSLHHAFFVGDILTKRDCLRLQVLANNVNVVNMYGTTET 609
+ P+ + L E + +++ A G+ L + L + N+YG +ET
Sbjct: 2862 INTVPSALKALLESGGLGEGVDTVNVA---GEALKRSLVETLFEQTQVKRLCNLYGPSET 2918
Query: 610 QRSVSYFVVPARSQDQTFLESQKDVIPA--GRGMKNVQLLVINRFDTNKICGIGEVGEIY 667
S+ + +++D A G+ + N Q +++ + + +G GEIY
Sbjct: 2919 TTYSSWVSM-----------AREDGFAAHIGKPVANTQFYLLD--EHKQPVPLGVPGEIY 2965
Query: 668 LRAGGLAEGYLGNDELTSKKFLKSWFADPSKFVDRTPENAPWKPYWFGIRDRMYRSGDLG 727
+ G+A GYL D+LT+++FLK DP V RMY++GDLG
Sbjct: 2966 IGGAGVARGYLNRDDLTAERFLK----DPFSAVPNA---------------RMYKTGDLG 3006
Query: 728 RYLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRENITLVRRDKDEEPTLVAY 787
RYLP GN+E GR DDQ+KIRGFRIELGEI L++H + E + L R D + LVAY
Sbjct: 3007 RYLPDGNIEYLGRNDDQVKIRGFRIELGEIEAKLAQHAALNETVVLAREDVPGDKRLVAY 3066
Query: 788 IVPQGLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKTKLPSYAIPSVIVPLHKMPL 847
S ES D I +R YL+ LPSY +P V L +PL
Sbjct: 3067 FTQH--------SPDESVD------------IEALRAYLQALLPSYMVPVAYVRLDALPL 3106
Query: 848 NPNGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERDIRDIWLRIIPHATDVNKKA 907
PNGK+D+ ALP PD A SR G + TE + IW ++ V +
Sbjct: 3107 TPNGKLDRKALPAPDLD--AVISR-----GYEAPQGETETTLAQIWQDLL-GLQQVGRHD 3158
Query: 908 SFFDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGLAKEI 952
FF++GGHS+LA LI +R+ ++ + ++F +PT+ LA +
Sbjct: 3159 HFFELGGHSLLAVTLIERMRQA-GLSADVRILFGQPTLAALAAAV 3202
Score = 242 bits (617), Expect = 1e-66
Identities = 199/661 (30%), Positives = 309/661 (46%), Gaps = 118/661 (17%)
Query: 314 TYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPP 373
TY +++ +N +AH L+ G+ D V + R +++VV ++GVLKAGA + +DPAYP
Sbjct: 3732 TYCELNARANQVAHRLLALGVCPDDRVAICVERSLEMVVGLLGVLKAGAGYVPVDPAYP- 3790
Query: 374 ARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKTYVPALKLAKDGSLTGGSVSK 433
A +I YL L+D+ ++ +V+ V + L VP + L G SVS
Sbjct: 3791 AERIAYL----------LQDSAPVA-VLVQAVTQGLLAAGAVPVINLDNAG-WQDESVSN 3838
Query: 434 GADDILQHVLHLKSEQTGVVVGPDSTPTLSFTSGSEGIPKGVKGRHFSLAYYFDWMAQEF 493
A V L++ V+ +TSGS G+PKGV H +L+ W Q F
Sbjct: 3839 PA------VPGLEARHLAYVI---------YTSGSTGLPKGVMVEHRNLSNLVGWHCQAF 3883
Query: 494 NLSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPTA----EDIGTPGQLAQWANKYKV 549
N+ R + ++G D +I+ L GA+L++P A ED+G L W +
Sbjct: 3884 NVKRGSRTSSVAGFGFDAAAWEIWPSLCAGATLLLPPAHAGSEDVGA---LLDWWQAQAL 3940
Query: 550 TVTHLTPAMGQLLAAQADEPIPSLHHAFF-------VGDILTKRDCLRLQVLANNVNVVN 602
V L P P +AF + +L D LR ++N
Sbjct: 3941 DVCFL--------------PTPIAEYAFGRNLGHDQLRTLLIGGDRLRKLPADLPFELIN 3986
Query: 603 MYGTTETQRSVSYFVVPARSQDQTFLESQKDVIPAGRGMKNVQLLVINRFDTNKICGIGE 662
YG TET VV + +++ + V+ G+ + N Q+ +++ + +G
Sbjct: 3987 NYGPTETT------VVATSGR----IDASQAVLHIGKPVANTQVYLLDAH--LQPVPVGV 4034
Query: 663 VGEIYLRAGGLAEGYLGNDELTSKKFLKSWFADPSKFVDRTPENAPWKPYWFGIRDRMYR 722
GE+Y+ G+A GYL +LT+++F+K DP V RMYR
Sbjct: 4035 AGELYIGGAGVARGYLNRGQLTAERFVK----DPFSLVQDA---------------RMYR 4075
Query: 723 SGDLGRYLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRENITLVRRDKDEEP 782
+GDLGRYLP GN++ GR D Q+KIRG RIELGEI L VRE + + + ++
Sbjct: 4076 TGDLGRYLPDGNIDYLGRNDSQLKIRGLRIELGEIEARLGACAGVREAVVVAVGEAPDDQ 4135
Query: 783 TLVAYIVPQGLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKTKLPSYAIPSVIVPL 842
LVAY D D A ++ +RE L+ LPS+ +P+ + L
Sbjct: 4136 RLVAYYTAH----DTLDQALTAD---------------SLREQLQVHLPSHMVPAAYMCL 4176
Query: 843 HKMPLNPNGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERDIRDIWLRIIPHATD 902
+PL PNGK+D ALP PD S ++S G E + IW ++
Sbjct: 4177 DALPLTPNGKLDHRALPVPD-------SEAYSGRGYAAPQGEVETALARIWSELL-KVEQ 4228
Query: 903 VNKKASFFDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGLAKEIERMKSGEMIS 962
V + +FF++GGHS++A LI E ++ ++ + ++FS+PT+ LA + SG +S
Sbjct: 4229 VGRYDNFFELGGHSLMAVSLI-ERMRQVGLSADVRVLFSQPTLAALAAAV---GSGSEVS 4284
Query: 963 V 963
V
Sbjct: 4285 V 4285
Score = 237 bits (604), Expect = 4e-65
Identities = 191/644 (29%), Positives = 297/644 (46%), Gaps = 91/644 (14%)
Query: 314 TYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPP 373
TY +++ +N LAH L+ GI D V + RG+D++V ++G+LK+GA + +DPA P
Sbjct: 8031 TYGELNAQANQLAHRLLSLGIRPDDRVAICVERGLDMLVGLLGILKSGAGYVPLDPASPA 8090
Query: 374 ARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKTYVPALKLAKDGSLTGGSVSK 433
R L + P A+VV L + E + +EL + PAL+ +
Sbjct: 8091 ERIAYMLEDSAPVAIVVHAATQAL---LAEESVRLIELDS--PALRSQSTAN-------- 8137
Query: 434 GADDILQHVLHLKSEQTGVVVGPDSTPTLSFTSGSEGIPKGVKGRHFSLAYYFDWMAQEF 493
V S Q V+ +TSGS G+PKGV H ++A F F
Sbjct: 8138 ------PQVPGQTSSQLAYVI---------YTSGSTGLPKGVMVEHRNVARLFSATQPWF 8182
Query: 494 NLSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPTAEDIGTPGQLAQWANKYKVTVTH 553
+ D + + A D +I+ L G L+V +P + + VTV +
Sbjct: 8183 EFGQQDVWALFHSFAFDFSVWEIWGALIHGGRLLVVPQLVSRSPQECYALLCQAGVTVLN 8242
Query: 554 LTP-AMGQLLAAQADEPIP-SLHHAFFVGDILTKRDCLR---LQVLANNVNVVNMYGTTE 608
TP A QL+ AQ + + SL F G+ L + L+ +V +VNMYG TE
Sbjct: 8243 QTPSAFRQLIVAQGESDLRHSLRQVIFGGEAL-ETAMLKPWYARVANAGTQLVNMYGITE 8301
Query: 609 TQRSVSYFVVPARSQDQTFLESQKDVIPAGRGMKNVQLLVINRFDTNKICGIGEVGEIYL 668
T V+Y + A T V P G+ + ++QL V++ + +G VGE+Y+
Sbjct: 8302 TTVHVTYRPLEAADAQLT------GVSPIGKRIPDLQLYVLDA--RREPVPVGVVGEMYV 8353
Query: 669 RAGGLAEGYLGNDELTSKKFLKSWFADPSKFVDRTPENAPWKPYWFGIRDRMYRSGDLGR 728
G+A GYL ELT ++F+ F+ G R+YR+GDLGR
Sbjct: 8354 GGAGVARGYLNRPELTQERFIADTFSG-------------------GEGARLYRTGDLGR 8394
Query: 729 YLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRENITLVRRDKDEEPTLVAYI 788
+L G++E GR DDQ+KIRGFRIELGEI L+ V + + + R D + LV Y+
Sbjct: 8395 WLADGSIEYLGRNDDQVKIRGFRIELGEIEATLAACEGVSDALVIAREDAPGDKRLVGYV 8454
Query: 789 VPQGLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKTKLPSYAIPSVIVPLHKMPLN 848
+ D A + L ++R L L Y +PS V L PL
Sbjct: 8455 IAA-------DGA--------------QLLAAELRAQLLASLADYMVPSAFVVLEAFPLT 8493
Query: 849 PNGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERDIRDIWLRIIPHATDVNKKAS 908
NGK+D+ ALP PD S +A + + + A +D+ D+ + + +
Sbjct: 8494 TNGKLDRKALPAPDQSAVATREYEAPQGETETVIAAIWQDLLDL--------ERIGRHDN 8545
Query: 909 FFDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGLAKEI 952
FF++GGHS+LA +L+ E + ++ + ++F +PT+ LA +
Sbjct: 8546 FFELGGHSLLAVKLL-ERMRHVGLSADVRVLFGQPTLAALAAAV 8588
Score = 228 bits (580), Expect = 3e-62
Identities = 185/645 (28%), Positives = 295/645 (45%), Gaps = 104/645 (16%)
Query: 314 TYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPP 373
+Y Q++E +N LAHHL+ G++ D V + R ++L+ + + +LK A + +D
Sbjct: 6956 SYAQLNEQANRLAHHLIGLGVQPDDCVAILLPRSIELLASQLAILKCAAAYVPLDRNASL 7015
Query: 374 ARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKTYVPALKLAKDGSLTGGSVSK 433
RQ L + + L+ + TV E + ++L T L G + ++++
Sbjct: 7016 ERQGFMLDDCQAKCLLTFS-----TETVPEGASR-IDLDT------LDSQGPVHNPALAQ 7063
Query: 434 GADDILQHVLHLKSEQTGVVVGPDSTPTLSFTSGSEGIPKGVKGRHFSLAY------YFD 487
++ I + +TSGS G PKGV H ++ Y D
Sbjct: 7064 SSESIAY---------------------IMYTSGSTGQPKGVLVPHRAINRLVINNGYAD 7102
Query: 488 WMAQEFNLSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPTAEDIGTPGQLAQWANKY 547
+ AQ DR S A D D++ L G ++V E + P + AQ
Sbjct: 7103 FNAQ-------DRIAFASNPAFDASTMDVWGALLNGGQVVVIDHETLLEPSRFAQVLQDS 7155
Query: 548 KVTVTHLTPAMGQLLAAQADEPIPSLHHAFFVGDILTKRDCLRLQVLANNVNVVNMYGTT 607
VTV +T A+ + + L G+ RL LA + +V+ YG T
Sbjct: 7156 GVTVLFVTTAIFNQYVQLIPQALGGLRILLCGGERADVASFRRLLDLAPGLRLVHCYGPT 7215
Query: 608 ETQRSVSYFVVPARSQDQTFLESQKDVIPAGRGMKNVQLLVINRFDTNKICGIGEVGEIY 667
ET + V A + D + +P G + N Q+ V++ ++ +G VGE+Y
Sbjct: 7216 ETTTYATTLEVKAVALD-------AECVPIGGPIGNTQVYVLDA--RQQLAPLGVVGEMY 7266
Query: 668 LRAGGLAEGYLGNDELTSKKFLKSWFADPSKFVDRTPENAPWKPYWFGIRDRMYRSGDLG 727
+ G+A+GYL +LT++KF+ ADP P+ +YR+GDLG
Sbjct: 7267 IGGQGVAKGYLNRPDLTAEKFI----ADP---FSHEPDAL------------LYRTGDLG 7307
Query: 728 RYLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRENITLVRRDKDEEPTLVAY 787
R+LP G++EC GR DDQ+KIRGFRIELGEI L VR+ + LVR D+ E LVAY
Sbjct: 7308 RWLPEGSLECLGRNDDQVKIRGFRIELGEIEAKLVACDGVRDAVVLVRADETGEKRLVAY 7367
Query: 788 IVPQGLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKTKLPSYAIPSVIVPLHKMPL 847
++ Q + V GL RE L + L Y +P+ V L PL
Sbjct: 7368 VIAQ-----------PQVTLSVAGL----------REQLSSTLSEYMVPAAFVMLPAFPL 7406
Query: 848 NPNGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERDIRDIWLRIIPHATDVNKKA 907
NGK+D+ ALP PD A+ + + + +++ L +W ++ + +
Sbjct: 7407 TLNGKVDRKALPAPDAEAYASQAYAAPQGEIEQVLAG-------MWAELL-KVERIGRHD 7458
Query: 908 SFFDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGLAKEI 952
FF++GGHS+LA LI +R+ ++ + ++FS+PT+ LA I
Sbjct: 7459 HFFELGGHSLLAVTLIERMRQA-GLSADVRVLFSQPTLAALAAAI 7502
Score = 226 bits (576), Expect = 8e-62
Identities = 191/654 (29%), Positives = 297/654 (45%), Gaps = 100/654 (15%)
Query: 312 SYTYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAY 371
S TY +++ +N LA HLV G+ GD V + R ++L+V+ + +LK A + +D +
Sbjct: 5877 SLTYAELNHRANRLARHLVGLGVRPGDRVAIALERSLELLVSQLAILKCAAVYVPLDVSA 5936
Query: 372 PPARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKTYVPALKLAKDGSLTGGSV 431
P RQ V A VVL ++P + V+ L T V
Sbjct: 5937 PLERQ--QFMVQDSGAQVVLTSGTAVAPEASQRVD----LDTLV---------------F 5975
Query: 432 SKGADDILQHVLHLKSEQTGVVVGPDSTPTLSFTSGSEGIPKGVKGRHFSL-AYYFDWMA 490
++ AD+ LHL Q+G +S + +TSGS G PKGV H ++ +
Sbjct: 5976 NEAADN-----LHLT--QSG-----ESVAYIMYTSGSTGTPKGVLVPHRAINRLVINNGY 6023
Query: 491 QEFNLSESDRFTMLSGIAHDPIQRDIFTPLFLGASLIVPTAEDIGTPGQLAQWANKYKVT 550
EFN DR S A D D++ PL G ++V + + T + A + V+
Sbjct: 6024 AEFNAQ--DRVAFASNPAFDASTLDVWAPLLNGGCVVVVDQDVLLTQERFAALLQEQSVS 6081
Query: 551 VTHLTPAMGQLLAAQADEPIPSLHHAFFVGDILTKRDCLRLQVLANNVNVVNMYGTTETQ 610
V +T + AA L + GD+L R+ ++++N YG TE
Sbjct: 6082 VLWMTAGLFHQYAAGLMSVFAQLRYLIVGGDVLDPAVIGRVLKEGAPLHLLNGYGPTEAT 6141
Query: 611 RSVSYFVVPARSQDQTFLESQKDVIPAGRGMKNVQLLVINRFDTNKICGIGEVGEIYLRA 670
+ + + + IP GR + N ++ V++ + IG GE+Y+
Sbjct: 6142 TFTTTHEIKSVGEGG---------IPIGRPIGNTRVYVLDA--NQQPVPIGVAGELYIGG 6190
Query: 671 GGLAEGYLGNDELTSKKFLKSWF-ADPSKFVDRTPENAPWKPYWFGIRDRMYRSGDLGRY 729
G+A+GYL EL+++KF+ F ADP +YR+GDL R+
Sbjct: 6191 DGVAKGYLNRPELSAEKFVADPFNADPGAL--------------------LYRTGDLARW 6230
Query: 730 LPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRENITLVRRDKDEEPTLVAYIV 789
G V+ GR DDQ+KIRGFRIELGEI L +H V++ + LVR D E LVAY
Sbjct: 6231 RADGTVDYLGRNDDQVKIRGFRIELGEIEARLGQHDEVKDVVVLVREDVPGEKRLVAYFT 6290
Query: 790 PQGLNKDDFDSATESEDIVVNGLKKYRKLIHDIREYLKTKLPSYAIPSVIVPLHKMPLNP 849
P+ D D A I +R +L+ +LP Y IP + L +PL
Sbjct: 6291 PR-----DLDVAPH---------------IETLRTHLQGQLPDYMIPVAYIRLDTLPLTA 6330
Query: 850 NGKIDKPALPFPDTSQLAAASRSHSKHGVDETLTATERDIRDIWLRIIPHATDVNKKASF 909
NGK+D+ ALP PD+ + + V++ L +W ++ V + +F
Sbjct: 6331 NGKLDRRALPAPDSEAYVSREYEAPQGEVEQLLA-------QLWAELL-RVEQVGRHDNF 6382
Query: 910 FDIGGHSILATRLIFELRKKFAVNVPLGLVFSEPTIEGLAKEIERMKSGEMISV 963
F++GGHS+LA LI E ++ ++ + ++F +PT+ LA I SG +SV
Sbjct: 6383 FELGGHSLLAVTLI-ERMRQVGLSADVRVLFGQPTLAALAAAI---GSGREVSV 6432
Lambda K H
0.318 0.136 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 25,409
Number of extensions: 1183
Number of successful extensions: 60
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 8
Length of query: 1419
Length of database: 8596
Length adjustment: 63
Effective length of query: 1356
Effective length of database: 8533
Effective search space: 11570748
Effective search space used: 11570748
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 67 (30.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory