GapMind for catabolism of small carbon sources

 

Aligments for a candidate for musK in Pseudomonas fluorescens FW300-N2C3

Align ABC-type maltose transporter (EC 7.5.2.1) (characterized)
to candidate AO356_28585 AO356_28585 ABC transporter

Query= BRENDA::Q8NMV1
         (376 letters)



>lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_28585 AO356_28585 ABC
           transporter
          Length = 379

 Score =  271 bits (692), Expect = 3e-77
 Identities = 144/304 (47%), Positives = 203/304 (66%), Gaps = 16/304 (5%)

Query: 21  VKKFNLEIADGEFLVLVGPSGCGKSTTLRMLAGLENVTDGAIFIGDKDVTHVAPRDRDIA 80
           ++  +LEI+ GEF+V VGPSGCGKST LR++AGL+++  G + I  + V  + PR+R + 
Sbjct: 19  LRDVSLEISAGEFVVFVGPSGCGKSTLLRLIAGLDSICGGDLLIDGRRVNDLEPRERGVG 78

Query: 81  MVFQNYALYPHMTVGENMGFALKIAGKSQDEINKRVDEAAATLGLTEFLERKPKALSGGQ 140
           MVFQ+YALYPHM+V +N+ F LK+A   +  + +RV + A  L L + L+RKP+ LSGGQ
Sbjct: 79  MVFQSYALYPHMSVYDNISFGLKLAKTEKTSLRERVLKTAQILQLDKLLQRKPRELSGGQ 138

Query: 141 RQRVAMGRAIVRNPQVFLMDEPLSNLDAKLRVQTRTQIAALQRKLGVTTVYVTHDQTEAL 200
           RQRVAMGRA+ R P + L DEPLSNLDA LRVQ R +IA L  +LG T +YVTHDQ EA+
Sbjct: 139 RQRVAMGRAMAREPDILLFDEPLSNLDASLRVQMRNEIARLHGRLGSTMIYVTHDQVEAM 198

Query: 201 TMGDRIAVLKDGYLQQVGAPRELYDRPANVFVAGFIGSPAMN-LGTFSVKDGDATS---- 255
           T+ D+I VL  G ++QVG+PRELY+RPA+ FVAGF+GSP MN L  F    G+ +     
Sbjct: 199 TLADKIVVLNGGRIEQVGSPRELYERPASRFVAGFLGSPRMNFLAAFLHTPGETSQVESL 258

Query: 256 --GHARIKLSPETLAAMTPEDNGRITIGFRPEALEIIPEGESTDLSIPIKLDFVEELGSD 313
             G   +      LAA     N ++++G RPE + +    ++   +  I +  VE LGS+
Sbjct: 259 VLGMTSLPFDSSGLAA-----NTQLSLGIRPEHIAL----KAAQGTAGIAVSGVEYLGSE 309

Query: 314 SFLY 317
           ++++
Sbjct: 310 TYVH 313


Lambda     K      H
   0.316    0.135    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 379
Number of extensions: 14
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 376
Length of database: 379
Length adjustment: 30
Effective length of query: 346
Effective length of database: 349
Effective search space:   120754
Effective search space used:   120754
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory