Alignments for a candidate for iolD in Pseudomonas fluorescens FW300-N2C3

GapMind for catabolism of small carbon sources

Alignments for a candidate for iolD in Pseudomonas fluorescens FW300-N2C3

Align 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione acylhydrolase (ring-opening) (EC 3.7.1.22) (characterized)
to candidate AO356_23185 AO356_23185 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione acylhydrolase (decyclizing)

Query= BRENDA::P42415
         (637 letters)



>FitnessBrowser__pseudo5_N2C3_1:AO356_23185
          Length = 643

 Score =  696 bits (1796), Expect = 0.0
 Identities = 349/639 (54%), Positives = 454/639 (71%), Gaps = 7/639 (1%)

Query: 6   RLTTAQALIKFLNQQYIHVDGKEEPFVEGIFTIFGHGNVLGIGQALEQDAGHLKVYQGKN 65
           RLT AQAL+KFL+ QY+ VDG +  FV G+FTIFGHGNVLG+GQALEQD+G L V+QG+N
Sbjct: 5   RLTMAQALVKFLDNQYVEVDGVQSKFVAGVFTIFGHGNVLGLGQALEQDSGDLVVHQGRN 64

Query: 66  EQGMAHAAMAYSKQMLRRKIYAVSTSVGPGAANLVAAAGTALANNIPVLLIPADTFATRQ 125
           EQGMAHAA+ ++KQ LRRKIYA S+SVGPGAAN++ AA TA AN IP+LL+P D +A+RQ
Sbjct: 65  EQGMAHAAIGFAKQHLRRKIYACSSSVGPGAANMLTAAATATANRIPLLLLPGDVYASRQ 124

Query: 126 PDPVLQQMEQEYSAAITTNDALKPVSRYWDRITRPEQLMSSLLRAFEVMTDPAKAGPATI 185
           PDPVLQQ+EQ +  +I+TNDA K VS+YWDRI RPEQLM++ ++A  V+TDPA+ G  T+
Sbjct: 125 PDPVLQQIEQFHDLSISTNDAFKAVSKYWDRINRPEQLMTAAIQAMRVLTDPAETGAVTL 184

Query: 186 CISQDVEGEAYDFDESFFVKRVHYIDRMQPSERELQGAAELIKSSKKPVILVGGGAKYSG 245
            + QDV+ EAYD+ + F  KRVH IDR   +E  L  A  L+K  ++P+I+ GGG +YSG
Sbjct: 185 ALPQDVQAEAYDYPDYFLQKRVHRIDRRPATEAMLGDARALLKGKRRPLIICGGGVRYSG 244

Query: 246 ARDELVAISEAYNIPLVETQAGKSTVEADFANNLGGMGITGTLAANKAARQADLIIGIGT 305
           A   L A +E ++IP  ETQAGKS V +    N+GG+G TG LAAN  A++ADLIIGIGT
Sbjct: 245 ANAALQAFAERFDIPFAETQAGKSAVVSSHPLNVGGVGETGCLAANLLAKKADLIIGIGT 304

Query: 306 RYTDFATSSKTAFDFDKAKFLNINVSRMQAYKLDAFQVVADAKVTLGKLHGLLEGYESEF 365
           RY+DF T SK  F     +FLN+N+S   A KLD  Q++ADA+  L  L   L  Y +E+
Sbjct: 305 RYSDFTTGSKWLFQHPDVQFLNLNISPCDALKLDGVQLLADARSGLESLSAALGDYRAEW 364

Query: 366 GTTIRELKDEWLAERERLSKVTFKREAFDPEIKNHFSQEVLNEYADALNTELPQTTALLT 425
           G  I + K +  AE +R+ +  ++ E F PEI +H    V  E+ +   + L Q+  L T
Sbjct: 365 GGQIADAKAQLDAEVDRIYQADYQAEEFLPEIDDHMDPAVFREFIELTGSCLTQSRVLGT 424

Query: 426 INETIPEDSVIICSAGSLPGDLQRLWHSNVPNTYHLEYGYSCMGYEVSGTLGLKLAHPDR 485
           +NET+ +D++I+ +AGSLPGDLQR W S   NTYH+EYGYSCMGYEV+  LG+KLA PD+
Sbjct: 425 LNETLADDAIIVAAAGSLPGDLQRAWRSKGVNTYHVEYGYSCMGYEVNAALGVKLAEPDK 484

Query: 486 EVYSIVGDGSFLMLHSELITAIQYNKKINVLLFDNSGFGCINNLQMDHGSGSYYCEFRTD 545
           EVY++VGDGS++MLHSEL T+IQ  +KINV+L DN  FGCINNLQM +G  S+  EFR  
Sbjct: 485 EVYALVGDGSYMMLHSELATSIQERRKINVVLLDNMAFGCINNLQMGNGMDSFGTEFRFR 544

Query: 546 -------DNQILNVDYAKVAEGYGAKTYRANTVEELKAALEDAKKQDVSTLIEMKVLPKT 598
                  D   + VD+A  A  YG KTY+A T+EEL+AAL DA++Q VSTLI++KVLPKT
Sbjct: 545 NPDTGKLDGDFVPVDFAMSAAAYGCKTYKATTLEELRAALADARRQTVSTLIDIKVLPKT 604

Query: 599 MTDGYDSWWHVGVAEVSEQESVQKAYEAKEKKLESAKQY 637
           M   Y SWW VGVA+VS         +   ++L  A+QY
Sbjct: 605 MIHSYLSWWRVGVAQVSTSARTDAVAKTLNERLAKARQY 643


Lambda     K      H
   0.316    0.132    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 975
Number of extensions: 35
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 637
Length of database: 643
Length adjustment: 38
Effective length of query: 599
Effective length of database: 605
Effective search space:   362395
Effective search space used:   362395
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources