Align BusAA, component of Uptake system for glycine-betaine (high affinity) and proline (low affinity) (OpuAA-OpuABC) or BusAA-ABC of Lactococcus lactis). BusAA, the ATPase subunit, has a C-terminal tandem cystathionine β-synthase (CBS) domain which is the cytoplasmic K+ sensor for osmotic stress (osmotic strength)while the BusABC subunit has the membrane and receptor domains fused to each other (Biemans-Oldehinkel et al., 2006; Mahmood et al., 2006; Gul et al. 2012). An N-terminal amphipathic α-helix of OpuA is necessary for high activity but is not critical for biogenesis or the ionic regulation of transport (characterized)
to candidate AO356_00615 AO356_00615 choline ABC transporter ATP-binding protein
Query= TCDB::Q9RQ06 (407 letters) >FitnessBrowser__pseudo5_N2C3_1:AO356_00615 Length = 407 Score = 288 bits (738), Expect = 2e-82 Identities = 172/391 (43%), Positives = 242/391 (61%), Gaps = 13/391 (3%) Query: 5 VKIEHLTKIFGKRIKTALTMVEQGEPKNEILKKTGATVGVYDTNFEINEGEIFVIMGLSG 64 ++ E + IF R K AL +++QG + EIL+KTG VGV N EIN+GEI V+MGLSG Sbjct: 4 IRFEDVDVIFAARPKPALELLDQGFSRPEILQKTGLIVGVEKANLEINKGEICVLMGLSG 63 Query: 65 SGKSTLLRLLNRLIEPTSGKIFIDDQ----DVATLNKEDLLQVRRKSMSMVFQNFGLFPH 120 SGKS+LLR +N L + GK+F++ + D+A+ +L +R + ++MVFQ F L P Sbjct: 64 SGKSSLLRCINGLNTVSRGKLFVEHEGRQIDIASCTPAELKMMRTQRIAMVFQKFALMPW 123 Query: 121 RTILENTEYGLEVQNVPKEERRKRAEKALDNANLLDFKDQYPKQLSGGMQQRVGLARALA 180 T+ EN +GLE+Q ++E RK ++ L+ L ++++ P +LSGGMQQRVGLARALA Sbjct: 124 LTVRENISFGLEMQGRSEKECRKLVDEKLELVGLTQWRNKKPDELSGGMQQRVGLARALA 183 Query: 181 NDPEILLMDEAFSALDPLIRREMQDELLELQAKFQKTIIFVSHDLNEALRIGDRIAIMKD 240 D +ILLMDE FSALDPLIR+ +QDELL+LQ K KTI+FVSHDL+EAL++G RIAIMKD Sbjct: 184 MDADILLMDEPFSALDPLIRQGLQDELLDLQRKLHKTIVFVSHDLDEALKLGTRIAIMKD 243 Query: 241 GKIMQIGTGEEILTNPANDYVKTFVEDVDRAKVITAENIMIPALTTNIDVDGPSVALKKM 300 GKI+Q T EEI+ NPA+DYV+ FV + V+ A+++M P +D +A + Sbjct: 244 GKIIQYSTPEEIVLNPADDYVRNFVAHTNPLNVLCAKSLMTP-------LDQCVLARDEY 296 Query: 301 KTEEVSSL-MAVDRKRQFRGVVTSEQAIAARKNNQSLK-DVMTTDVGTVTKEMLVRDILP 358 + +L M V+ +R V + N + + D + V +RD L Sbjct: 297 CLDTAQNLWMVVNPQRHLTSVRKGATTAHVQSWNPTERIDTLARQPTCVPPITTMRDALQ 356 Query: 359 IIYDAPTPLAVVDDQGYLKGILIRGIVLEAL 389 I Y+ PL V D+ + GIL G + AL Sbjct: 357 IRYETGHPLIVRDEADTVLGILGDGELYRAL 387 Lambda K H 0.316 0.135 0.364 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 380 Number of extensions: 13 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 1 Length of query: 407 Length of database: 407 Length adjustment: 31 Effective length of query: 376 Effective length of database: 376 Effective search space: 141376 Effective search space used: 141376 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory