Align RhaT, component of Rhamnose porter (Richardson et al., 2004) (Transport activity is dependent on rhamnokinase (RhaK; AAQ92412) activity (Richardson and Oresnik, 2007) This could be an example of group translocation!) (characterized)
to candidate AO356_00965 AO356_00965 sugar ABC transporter ATP-binding protein
Query= TCDB::Q7BSH4 (512 letters) >FitnessBrowser__pseudo5_N2C3_1:AO356_00965 Length = 517 Score = 287 bits (735), Expect = 6e-82 Identities = 175/499 (35%), Positives = 279/499 (55%), Gaps = 12/499 (2%) Query: 16 DAPAILEMRGISQIFPGVKALDNVSIALHPGTVTALIGENGAGKSTLVKILTGIYRPNEG 75 D A+L + GI + + L ++++ L G V AL GENGAGKSTL KI+ G+ P G Sbjct: 5 DPNAVLCVSGIGKTY-AQPVLTDINLTLMRGEVLALTGENGAGKSTLSKIIGGLVTPTTG 63 Query: 76 EILVDGRPTTFASAQAAIDAGVTAIHQETVLFDELTVAENIFLGHAPRTRFRTIDWQTMN 135 ++ G+ S A + GV + QE L L+VAEN+FL + P W + Sbjct: 64 QMQFQGQDYRPGSRTQAEELGVRMVMQELNLLPTLSVAENLFLDNLPSHG----GWISRK 119 Query: 136 SRSKALLTALES----NIDPTIRLKDLSIAQRHLVAIARALSIEARIVIMDEPTAALSRK 191 KA + A+ IDP + +L I + +V IAR L + ++I+DEPTA L+ + Sbjct: 120 QLRKAAIEAMAQVGLDAIDPDTLVGELGIGHQQMVEIARNLIGDCHVLILDEPTAMLTAR 179 Query: 192 EIDDLFRIVRGLKEQGKAILFISHKFDELYEIADDFVVFPRRSRRPVRGVSRKTPQDEIV 251 E++ LF + L+ +G AI++ISH+ +EL +A V + V ++ +++V Sbjct: 180 EVEMLFEQITRLQARGVAIIYISHRLEELARVAQRIAVLRDGNLVCVEPMANYD-SEQLV 238 Query: 252 RMMVGRDVENVFPKIDVAIGGPVLEIRNYSHRTEFRDISFTLRKGEILGVYGLIGAGRSE 311 +MVGR++ IG P L ++ + + RD+SF +R GEI G+ GLIGAGR+E Sbjct: 239 TLMVGRELGEHIDLGPRQIGAPALTVKGLTRSDKVRDVSFEVRSGEIFGISGLIGAGRTE 298 Query: 312 LSQSLFGITKPLSGKMVL--EGQEITIHSPQDAIRAGIVYVPEERGRHGLALPMPIFQNM 369 L + +FG SG + L + ++I SP DA+ GI + E+R GL L I N+ Sbjct: 299 LLRLIFGADPADSGTVALGSPARVVSIRSPSDAVAHGIALITEDRKGEGLLLTQSIAANI 358 Query: 370 TLPSLARTSRRGFLRAANEFALARKYAERLDLRAAALSVPVGTLSGGNQQKVVIGKWLAT 429 L ++ S G + + E ALA++ + + +R+++ + V LSGGNQQKVVIG+WL Sbjct: 359 ALGNMPEISSAGLVNGSAELALAQRQVDAMRIRSSSPTQLVSELSGGNQQKVVIGRWLER 418 Query: 430 APKVIILDEPTKGIDIGSKAAVHGFISELAAEGLSIIMVSSELPEIIGMSDRVLVMKEGL 489 V++ DEPT+GID+G+K ++ + EL +G ++++VSS+L E++ + DR+ V+ G Sbjct: 419 DCAVMLFDEPTRGIDVGAKFDIYALLGELTRQGKALVVVSSDLRELMLICDRIGVLSAGR 478 Query: 490 SAGIFERAELSPEALVRAA 508 FER + + L+ AA Sbjct: 479 LIDTFERDSWTQDDLLAAA 497 Score = 86.7 bits (213), Expect = 2e-21 Identities = 67/223 (30%), Positives = 108/223 (48%), Gaps = 5/223 (2%) Query: 288 DISFTLRKGEILGVYGLIGAGRSELSQSLFGITKPLSGKMVLEGQEITIHSPQDAIRAGI 347 DI+ TL +GE+L + G GAG+S LS+ + G+ P +G+M +GQ+ S A G+ Sbjct: 26 DINLTLMRGEVLALTGENGAGKSTLSKIIGGLVTPTTGQMQFQGQDYRPGSRTQAEELGV 85 Query: 348 VYVPEERGRHGLALPMPIFQNMTLPSLARTSRRGFLRAANEFALARKYAERLDLRAAALS 407 V +E L + + +N+ L +L S G++ A + ++ L A Sbjct: 86 RMVMQELN---LLPTLSVAENLFLDNLP--SHGGWISRKQLRKAAIEAMAQVGLDAIDPD 140 Query: 408 VPVGTLSGGNQQKVVIGKWLATAPKVIILDEPTKGIDIGSKAAVHGFISELAAEGLSIIM 467 VG L G+QQ V I + L V+ILDEPT + + I+ L A G++II Sbjct: 141 TLVGELGIGHQQMVEIARNLIGDCHVLILDEPTAMLTAREVEMLFEQITRLQARGVAIIY 200 Query: 468 VSSELPEIIGMSDRVLVMKEGLSAGIFERAELSPEALVRAATG 510 +S L E+ ++ R+ V+++G + A E LV G Sbjct: 201 ISHRLEELARVAQRIAVLRDGNLVCVEPMANYDSEQLVTLMVG 243 Lambda K H 0.320 0.137 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 616 Number of extensions: 29 Number of successful extensions: 7 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 2 Length of query: 512 Length of database: 517 Length adjustment: 35 Effective length of query: 477 Effective length of database: 482 Effective search space: 229914 Effective search space used: 229914 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory