Alignments for a candidate for gcvT in Pseudomonas fluorescens FW300-N2C3

GapMind for catabolism of small carbon sources

Alignments for a candidate for gcvT in Pseudomonas fluorescens FW300-N2C3

Align Aminomethyltransferase (EC 2.1.2.10) (characterized)
to candidate AO356_18160 AO356_18160 glycine cleavage system protein T

Query= reanno::pseudo6_N2E2:Pf6N2E2_5556
         (374 letters)



>FitnessBrowser__pseudo5_N2C3_1:AO356_18160
          Length = 374

 Score =  723 bits (1866), Expect = 0.0
 Identities = 361/374 (96%), Positives = 371/374 (99%)

Query: 1   MSTEQLLKTPLHALHLELGARMVPFAGYDMPVQYPLGVMKEHQHTRDQAGLFDVSHMGQI 60
           MSTEQLLKTPLHALHLELGARMVPFAGYDMPVQYPLGVMKEHQHTRDQAGLFDVSHMGQI
Sbjct: 1   MSTEQLLKTPLHALHLELGARMVPFAGYDMPVQYPLGVMKEHQHTRDQAGLFDVSHMGQI 60

Query: 61  RLTGAGAAKALETLVPVDIIDLPVGMQRYAMFTNENGGILDDLMVANLGNDELFLVVNAA 120
           RL+GAGAAKALETLVPVDIIDLPVGMQRYAMFTN+NGGILDDLMVANLGNDELFLVVNAA
Sbjct: 61  RLSGAGAAKALETLVPVDIIDLPVGMQRYAMFTNDNGGILDDLMVANLGNDELFLVVNAA 120

Query: 121 CKDQDLAHLRAHIGAQCSIEPLFEARALLALQGPAAVTVLARLAPDVAKMTFMQFQRVTL 180
           CKDQDLAHLRAHIG QC+IEPLFEARALLALQGPAAVTVLARLAPDVAKMTFMQFQRVTL
Sbjct: 121 CKDQDLAHLRAHIGDQCNIEPLFEARALLALQGPAAVTVLARLAPDVAKMTFMQFQRVTL 180

Query: 181 LGMDCYVSRSGYTGEDGFEISVPAAEAEKLARALLAEPEVAAIGLGARDSLRLEAGLCLY 240
           LG+DC+VSRSGYTGEDGFEISVPAA+AEKLARALLAEPEVAAIGLGARDSLRLEAGLCLY
Sbjct: 181 LGVDCFVSRSGYTGEDGFEISVPAADAEKLARALLAEPEVAAIGLGARDSLRLEAGLCLY 240

Query: 241 GHDMNTETTPIEASLLWAISKVRRADGARAGGFPGAETVFAQQQGGVKRKRVGLLPQERT 300
           GHDMNTETTPIEASLLWAISKVRRADG RAGGFPGAE+VFAQQQGGVKRKRVGLLPQERT
Sbjct: 241 GHDMNTETTPIEASLLWAISKVRRADGGRAGGFPGAESVFAQQQGGVKRKRVGLLPQERT 300

Query: 301 PVREGAEIVNEAGEIIGTVCSGGFGPTLGGPLAMGYLDSAYVALDTPVWAIVRGKKVPLL 360
           PVREGAEIVN+AGEIIG+VCSGGFGPTLG PLAMGYLDSAYVALDTPVWAIVRGK+VPLL
Sbjct: 301 PVREGAEIVNDAGEIIGSVCSGGFGPTLGAPLAMGYLDSAYVALDTPVWAIVRGKRVPLL 360

Query: 361 VSKMPFVPQRYYRG 374
           VSKMPFVPQRYYRG
Sbjct: 361 VSKMPFVPQRYYRG 374


Lambda     K      H
   0.321    0.138    0.406 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 669
Number of extensions: 29
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 374
Length of database: 374
Length adjustment: 30
Effective length of query: 344
Effective length of database: 344
Effective search space:   118336
Effective search space used:   118336
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

Align candidate AO356_18160 AO356_18160 (glycine cleavage system protein T)
to HMM TIGR00528 (gcvT: glycine cleavage system T protein (EC 2.1.2.10))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR00528.hmm
# target sequence database:        /tmp/gapView.11509.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00528  [M=362]
Accession:   TIGR00528
Description: gcvT: glycine cleavage system T protein
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                       Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                       -----------
   2.6e-109  351.4   0.0     3e-109  351.2   0.0    1.0  1  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_18160  AO356_18160 glycine cleavage sys


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_18160  AO356_18160 glycine cleavage system protein T
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  351.2   0.0    3e-109    3e-109       2     361 ..       7     368 ..       6     369 .. 0.97

  Alignments for each domain:
  == domain 1  score: 351.2 bits;  conditional E-value: 3e-109
                                       TIGR00528   2 krtpLydlhtelggklvdfaGwelPvqyk.slieeheavrtkaGlfDvshmgkvelsGskslk 63 
                                                      +tpL+ lh elg+++v+faG+ +Pvqy  ++++eh+ +r++aGlfDvshmg+++lsG  + k
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_18160   7 LKTPLHALHLELGARMVPFAGYDMPVQYPlGVMKEHQHTRDQAGLFDVSHMGQIRLSGAGAAK 69 
                                                     79**************************6488******************************* PP

                                       TIGR00528  64 fLqrllanDvdaLtkGkaqysvllnaqGGvvDDlivykegednrfllvvnaataekDlewlke 126
                                                     +L+ l++ D+  L++G  +y+ + n +GG++DDl+v + g+d ++ lvvnaa++++Dl++l+ 
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_18160  70 ALETLVPVDIIDLPVGMQRYAMFTNDNGGILDDLMVANLGND-ELFLVVNAACKDQDLAHLRA 131
                                                     ******************************************.999***************** PP

                                       TIGR00528 127 hleeevtldtlskeisllalqGPkaktiledlldkaveglkefffvqeaelalkkaliartGy 189
                                                     h+ ++ +++ l +  +llalqGP a t+l  l   +v ++  f+  q+  l++ +++++r+Gy
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_18160 132 HIGDQCNIEPLFEARALLALQGPAAVTVLARLAP-DVAKM-TFMQFQRVTLLGVDCFVSRSGY 192
                                                     *****************************99875.66676.47777899************** PP

                                       TIGR00528 190 tGedGfeiavanekavelwkklveaygvkPiGLgarDtLrleagmaLyGqeldeeitPleagl 252
                                                     tGedGfei+v+  +a +l+++l++++ v  iGLgarD+Lrleag++LyG++++ e+tP+ea+l
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_18160 193 TGEDGFEISVPAADAEKLARALLAEPEVAAIGLGARDSLRLEAGLCLYGHDMNTETTPIEASL 255
                                                     *************************************************************** PP

                                       TIGR00528 253 gwvvkker.....kksdfiGravleeqkengtekklvGlemlekgiarnelkvlltngekevG 310
                                                      w+++k r     +   f G + +  q++ g+++k+vGl   e+   r++ +++  ++ +++G
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_18160 256 LWAISKVRradggRAGGFPGAESVFAQQQGGVKRKRVGLLPQERTPVREGAEIVN-DAGEIIG 317
                                                     *****998888888899**************************************.777**** PP

                                       TIGR00528 311 ivtsGtlsPtLgknialayvdkelekiGtklevevrnklvkikvvkklfvr 361
                                                      v sG + PtLg  +a++y+d+ +    t +   vr+k v++ v k++fv+
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_18160 318 SVCSGGFGPTLGAPLAMGYLDSAYVALDTPVWAIVRGKRVPLLVSKMPFVP 368
                                                     *************************************************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (362 nodes)
Target sequences:                          1  (374 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 8.70
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources