Alignments for a candidate for gloB in Pseudomonas fluorescens FW300-N2C3

GapMind for catabolism of small carbon sources

Alignments for a candidate for gloB in Pseudomonas fluorescens FW300-N2C3

Align hydroxyacylglutathione hydrolase (EC 3.1.2.6) (characterized)
to candidate AO356_24480 AO356_24480 hydroxyacylglutathione hydrolase

Query= BRENDA::Q8ZRM2
         (251 letters)



>FitnessBrowser__pseudo5_N2C3_1:AO356_24480
          Length = 255

 Score =  226 bits (577), Expect = 3e-64
 Identities = 118/255 (46%), Positives = 161/255 (63%), Gaps = 5/255 (1%)

Query: 1   MNLNSIPAFQDNYIWVLTNDEG-RCVIVDPGEAAPVLKAIAEHK-WMPEAIFLTHHHHDH 58
           + ++++PAF DNYIW+L +    RC +VDPG+AAPV   +  H  W+   I +THHHHDH
Sbjct: 2   IQISALPAFTDNYIWLLQDHSTQRCAVVDPGDAAPVQAWLDAHPGWVLSDILITHHHHDH 61

Query: 59  VGGVKELLQHFPQMTVYGPAETQDKGATHLVGDGDTIRVLGEKFTLFATPGHTLGHVCYF 118
           VGGV + L++    TVYGPA          + D D + VLG  F ++A PGHTLGH+ Y+
Sbjct: 62  VGGV-QALKNATNATVYGPASENIPARDQALHDNDQVSVLGWDFDVYAVPGHTLGHIAYY 120

Query: 119 SRPYLFCGDTLFSGGCGRLFEGTPSQMYQSLMKINSLPDDTLICCAHEYTLANIKFALSI 178
               LFCGDTLF+ GCGRLFEGTP+QM+ SL ++ +LP+DTL+ C HEYTL+N+KFA ++
Sbjct: 121 HHGLLFCGDTLFAAGCGRLFEGTPAQMHHSLGRLAALPEDTLVYCTHEYTLSNLKFAAAV 180

Query: 179 LPHDSFINEYYRKVKELRVKKQMTLPVILKNERKINLFLRTEDIDLINEINKET--ILQQ 236
            P +  I     KV   R +  MTLP  L  E+  N FLRT +  +  + ++ +    Q 
Sbjct: 181 EPGNPDIAARLEKVSRQRNEGIMTLPSTLALEKLTNPFLRTTETSVKQKADERSGQRNQT 240

Query: 237 PEARFAWLRSKKDTF 251
           P   FA LR+ KDTF
Sbjct: 241 PSEVFAALRAWKDTF 255


Lambda     K      H
   0.323    0.140    0.436 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 259
Number of extensions: 13
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 251
Length of database: 255
Length adjustment: 24
Effective length of query: 227
Effective length of database: 231
Effective search space:    52437
Effective search space used:    52437
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 46 (22.3 bits)

Align candidate AO356_24480 AO356_24480 (hydroxyacylglutathione hydrolase)
to HMM TIGR03413 (gloB: hydroxyacylglutathione hydrolase (EC 3.1.2.6))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR03413.hmm
# target sequence database:        /tmp/gapView.15627.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR03413  [M=248]
Accession:   TIGR03413
Description: GSH_gloB: hydroxyacylglutathione hydrolase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                       Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                       -----------
   5.3e-109  349.3   0.0     6e-109  349.2   0.0    1.0  1  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_24480  AO356_24480 hydroxyacylglutathio


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_24480  AO356_24480 hydroxyacylglutathione hydrolase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  349.2   0.0    6e-109    6e-109       1     248 []       4     255 .]       4     255 .] 0.98

  Alignments for each domain:
  == domain 1  score: 349.2 bits;  conditional E-value: 6e-109
                                       TIGR03413   1 iiaipalsdNyiwllkdeks.eavvvDpgeaepvlealeek.glkleaillTHhHaDHvggva 61 
                                                     i a+pa++dNyiwll+d+++ +++vvDpg+a+pv+++l+++ g+ l++il+THhH+DHvggv+
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_24480   4 ISALPAFTDNYIWLLQDHSTqRCAVVDPGDAAPVQAWLDAHpGWVLSDILITHHHHDHVGGVQ 66 
                                                     5789**************999*****************99879******************** PP

                                       TIGR03413  62 ellekfpvkvvgpaeeripgltkevkegdevellelevevlevpGHtlgHiayyleeekvlFc 124
                                                     +l+++++++v+gpa+e+ip+ +++++++d+v++l+ +++v++vpGHtlgHiayy +  ++lFc
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_24480  67 ALKNATNATVYGPASENIPARDQALHDNDQVSVLGWDFDVYAVPGHTLGHIAYYHH--GLLFC 127
                                                     ********************************************************..***** PP

                                       TIGR03413 125 gDtLfsaGCGrlfegtaeqmleslqklaaLpeetkvycaHEYtlsNlrFalavepenealker 187
                                                     gDtLf+aGCGrlfegt++qm++sl +laaLpe+t+vyc+HEYtlsNl+Fa+avep n+++++r
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_24480 128 GDTLFAAGCGRLFEGTPAQMHHSLGRLAALPEDTLVYCTHEYTLSNLKFAAAVEPGNPDIAAR 190
                                                     *************************************************************** PP

                                       TIGR03413 188 lkevealrakgkptlPstlaeekatNpFLraeeaevkaalee....ekaeevevfaelRekkd 246
                                                     l++v+++r++g +tlPstla ek tNpFLr+ e++vk++++e    +++++ evfa+lR++kd
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_24480 191 LEKVSRQRNEGIMTLPSTLALEKLTNPFLRTTETSVKQKADErsgqRNQTPSEVFAALRAWKD 253
                                                     ****************************************9999988999************* PP

                                       TIGR03413 247 kf 248
                                                     +f
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_24480 254 TF 255
                                                     98 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (248 nodes)
Target sequences:                          1  (255 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 6.38
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources