Align acetoacetate-CoA ligase (EC 6.2.1.16) (characterized)
to candidate AO356_00425 AO356_00425 acetoacetyl-CoA synthetase
Query= BRENDA::Q9Z3R3
(650 letters)
>FitnessBrowser__pseudo5_N2C3_1:AO356_00425
Length = 651
Score = 686 bits (1769), Expect = 0.0
Identities = 353/649 (54%), Positives = 442/649 (68%), Gaps = 7/649 (1%)
Query: 7 LWVPDREIVERSPMAEFIDWCGERFGRSFADYDAFHDWSVSERGAFWTAVWEHCKV-IGE 65
LW P E + +S M F + R DY H WS+ +R AFW A+ + ++
Sbjct: 5 LWQPSPERIAQSRMNAFRRFVNARHHLQLNDYPTLHAWSIDQREAFWQAIVDFFEIRFHH 64
Query: 66 SGEKALVDGDRMLDARFFPEARLNFAENLLRKTGSGDALIFRGEDKVSYRLTWDELRALV 125
+ + LV+G +M A++FP A LNFAE+LLR+ A++ GE+ +LTW EL A V
Sbjct: 65 APDAVLVEGAQMPSAQWFPGATLNFAEHLLRRRDDAVAVVALGENGGQEQLTWAELAAHV 124
Query: 126 SRLQQALRAQGIGAGDRVAAMMPNMPETIALMLATASVGAIWSSCSPDFGEQGVLDRFGQ 185
+ LQ++L+A G+ GDRVAA MPN +T+ MLA+ S+GAIWS SPDFG QGV+DRFGQ
Sbjct: 125 AGLQKSLQAAGVTRGDRVAACMPNTWQTLVGMLASTSLGAIWSCSSPDFGTQGVIDRFGQ 184
Query: 186 IAPKLFIVCDGYWYNGKRQDVDSKVRAVAKSLGA--PTVIVPYAGDSAALAP--TVEGGV 241
I PK+ I C GY Y GK D +KV + + L + +++PYA A L T
Sbjct: 185 IEPKVLITCAGYRYAGKVIDQRAKVNEILERLPSLQQLIVLPYAQPDARLEDFKTPAAIA 244
Query: 242 TLADFIAGFQAGPLVFERLPFGHPLYILFSSGTTGVPKCIVHSAGGTLLQHLKEHRFHCG 301
DF + G F +PF HPLYIL+SSGTTGVPKCIVH GG LLQH+KEH H
Sbjct: 245 LWNDFYQ--RGGEPAFVSVPFDHPLYILYSSGTTGVPKCIVHGTGGVLLQHVKEHGLHVD 302
Query: 302 LRDGERLFYFTTCGWMMWNWLASGLAVGATLCLYDGSPFCPDGNVLFDYAAAERFAVFGT 361
L +RLFY+TTCGWMMWNWL S LAVG+ + LYDGSPF P L D +R +VFGT
Sbjct: 303 LGPEDRLFYYTTCGWMMWNWLVSALAVGSAVVLYDGSPFHPGPERLLDLIDQQRISVFGT 362
Query: 362 SAKYIDAVRKGGFTPARTHDLSSLRLMTSTGSPLSPEGFSFVYEGIKPDVQLASISGGTD 421
S K++ A+ G P +TH L SL+ + STGS L+P + +VY +KPDV LAS+SGGTD
Sbjct: 363 SPKFLAALESEGLKPRQTHRLDSLKTLLSTGSALAPHSYDYVYRDVKPDVCLASMSGGTD 422
Query: 422 IVSCFVLGNPLKPVWRGEIQGPGLGLAVDVWNDEGKPVRGEKGELVCTRAFPSMPVMFWN 481
IVSCFV GNPL PV RGE+QG LG+AV VWN+ G PV GEKGELVCTR FP+MP+ WN
Sbjct: 423 IVSCFVNGNPLSPVRRGEMQGKSLGMAVQVWNEAGNPVIGEKGELVCTRHFPAMPIGLWN 482
Query: 482 DPDGAKYRAAYFDRFDNVWCHGDFAEWTPHGGIVIHGRSDATLNPGGVRIGTAEIYNQVE 541
DP K RA+YF +F VW GD+AE PHGG++IHGRSDA LNPGGVRIGTAEIY QVE
Sbjct: 483 DPRQEKLRASYFAQFPGVWAQGDYAEELPHGGLLIHGRSDAVLNPGGVRIGTAEIYRQVE 542
Query: 542 QMDEVAEALCIGQDWEDDVRVVLFVRLARGVELTEALTREIKNRIRSGASPRHVPAKIIA 601
++++V +++ IGQ W+ DVRVVLFVRL GV L +AL + I+ IR+ +PRHVPAKI+A
Sbjct: 543 KVEQVLDSIAIGQQWQGDVRVVLFVRLRDGVTLDQALEQRIRQVIRTHTTPRHVPAKILA 602
Query: 602 VADIPRTKSGKIVELAVRDVVHGRPVKNKEALANPEALDLFAGLEELKS 650
V+DIPRT SGK+VELAVR+VVHG VKN +ALANPEAL+ F EL S
Sbjct: 603 VSDIPRTISGKVVELAVRNVVHGEVVKNTDALANPEALEQFRDRPELAS 651
Lambda K H
0.322 0.139 0.441
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1380
Number of extensions: 72
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 650
Length of database: 651
Length adjustment: 38
Effective length of query: 612
Effective length of database: 613
Effective search space: 375156
Effective search space used: 375156
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 54 (25.4 bits)
Align candidate AO356_00425 AO356_00425 (acetoacetyl-CoA synthetase)
to HMM TIGR01217 (acetoacetate-CoA ligase (EC 6.2.1.16))
# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file: ../tmp/path.carbon/TIGR01217.hmm
# target sequence database: /tmp/gapView.17219.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Query: TIGR01217 [M=652]
Accession: TIGR01217
Description: ac_ac_CoA_syn: acetoacetate-CoA ligase
Scores for complete sequences (score includes all domains):
--- full sequence --- --- best 1 domain --- -#dom-
E-value score bias E-value score bias exp N Sequence Description
------- ------ ----- ------- ------ ----- ---- -- -------- -----------
2.3e-268 877.6 0.0 2.5e-268 877.5 0.0 1.0 1 lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_00425 AO356_00425 acetoacetyl-CoA synt
Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_00425 AO356_00425 acetoacetyl-CoA synthetase
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 877.5 0.0 2.5e-268 2.5e-268 5 650 .. 4 649 .. 1 651 [] 0.98
Alignments for each domain:
== domain 1 score: 877.5 bits; conditional E-value: 2.5e-268
TIGR01217 5 vlwepdaervkdarlarfraavgerfGaalgdydalyrwsvdeldafwkavwefsdvvfssae 67
lw+p er++++r++ fr +v r+ + l dy l+ ws+d+++afw+a+++f++++f++a
lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_00425 4 LLWQPSPERIAQSRMNAFRRFVNARHHLQLNDYPTLHAWSIDQREAFWQAIVDFFEIRFHHAP 66
69*********************************************************9877 PP
TIGR01217 68 kev.vddskmlaarffpgarlnyaenllrkkgsedallyvdeekesakvtfeelrrqvaslaa 129
++v v++ +m++a++fpga+ln+ae+llr+++ a++ +e+ + ++t++el + va l++
lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_00425 67 DAVlVEGAQMPSAQWFPGATLNFAEHLLRRRDDAVAVVALGENGGQEQLTWAELAAHVAGLQK 129
7652899******************************************************** PP
TIGR01217 130 alralGvkkGdrvagylpnipeavaallatasvGaiwsscspdfGargvldrfsqiepkllfs 192
+l+a Gv +Gdrva+ +pn+ ++++ +la++s+Gaiws +spdfG++gv+drf+qiepk+l++
lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_00425 130 SLQAAGVTRGDRVAACMPNTWQTLVGMLASTSLGAIWSCSSPDFGTQGVIDRFGQIEPKVLIT 192
*************************************************************** PP
TIGR01217 193 vdgyvynGkehdrrekvrevakelpdlravvlipyvgdreklap.kvegalt.ledllaaaqa 253
+ gy+y Gk d+r kv+e+ lp+l++ +++py+ + ++l+ k ++a+ ++d+
lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_00425 193 CAGYRYAGKVIDQRAKVNEILERLPSLQQLIVLPYAQPDARLEDfKTPAAIAlWNDFYQR--G 253
*************************************99987666999998637999876..9 PP
TIGR01217 254 aelvfeqlpfdhplyilfssGttGvpkaivhsaGGtlvqhlkehvlhcdltdgdrllyyttvG 316
+e+ f +pfdhplyil+ssGttGvpk+ivh +GG+l+qh+keh+lh dl++ drl+yytt+G
lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_00425 254 GEPAFVSVPFDHPLYILYSSGTTGVPKCIVHGTGGVLLQHVKEHGLHVDLGPEDRLFYYTTCG 316
9************************************************************** PP
TIGR01217 317 wmmwnflvsglatGatlvlydGsplvpatnvlfdlaeregitvlGtsakyvsavrkkglkpar 379
wmmwn+lvs+la+G+++vlydGsp+ p ++ l dl+++++i+v+Gts k++ a++ +glkp +
lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_00425 317 WMMWNWLVSALAVGSAVVLYDGSPFHPGPERLLDLIDQQRISVFGTSPKFLAALESEGLKPRQ 379
*************************************************************** PP
TIGR01217 380 thdlsalrlvastGsplkpegfeyvyeeikadvllasisGGtdivscfvganpslpvykGeiq 442
th l++l++++stGs l p++++yvy+ +k+dv las+sGGtdivscfv +np+ pv +Ge+q
lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_00425 380 THRLDSLKTLLSTGSALAPHSYDYVYRDVKPDVCLASMSGGTDIVSCFVNGNPLSPVRRGEMQ 442
*************************************************************** PP
TIGR01217 443 apglGlaveawdeeGkpvtgekGelvvtkplpsmpvrfwndedGskyrkayfdkypgvwahGd 505
+++lG+av++w+e G+pv gekGelv+t+ +p+mp+ +wnd+ k r++yf ++pgvwa Gd
lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_00425 443 GKSLGMAVQVWNEAGNPVIGEKGELVCTRHFPAMPIGLWNDPRQEKLRASYFAQFPGVWAQGD 505
*************************************************************** PP
TIGR01217 506 yieltprGgivihGrsdatlnpnGvrlGsaeiynaverldeveeslvigqeqedgeervvlfv 568
y+e p+Gg++ihGrsda lnp+Gvr+G+aeiy +ve++++v +s+ igq+++ g++rvvlfv
lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_00425 506 YAEELPHGGLLIHGRSDAVLNPGGVRIGTAEIYRQVEKVEQVLDSIAIGQQWQ-GDVRVVLFV 567
***************************************************98.5******** PP
TIGR01217 569 klasGatldealvkeikdairaglsprhvpskiievagiprtlsGkkvevavkdvvaGkpven 631
+l G+tld+al ++i++ ir+ +prhvp+ki++v++iprt+sGk ve+av++vv+G+ v+n
lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_00425 568 RLRDGVTLDQALEQRIRQVIRTHTTPRHVPAKILAVSDIPRTISGKVVELAVRNVVHGEVVKN 630
*************************************************************** PP
TIGR01217 632 kgalsnpealdlyeeleel 650
+al+npeal+ +++ +el
lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_00425 631 TDALANPEALEQFRDRPEL 649
**************98887 PP
Internal pipeline statistics summary:
-------------------------------------
Query model(s): 1 (652 nodes)
Target sequences: 1 (651 residues searched)
Passed MSV filter: 1 (1); expected 0.0 (0.02)
Passed bias filter: 1 (1); expected 0.0 (0.02)
Passed Vit filter: 1 (1); expected 0.0 (0.001)
Passed Fwd filter: 1 (1); expected 0.0 (1e-05)
Initial search space (Z): 1 [actual number of targets]
Domain search space (domZ): 1 [number of targets reported over threshold]
# CPU time: 0.04u 0.02s 00:00:00.06 Elapsed: 00:00:00.05
# Mc/sec: 8.42
//
[ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory