Align 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) (characterized)
to candidate AO356_30360 AO356_30360 3-hydroxypropionyl-CoA dehydratase
Query= reanno::pseudo1_N1B4:Pf1N1B4_4790 (356 letters) >FitnessBrowser__pseudo5_N2C3_1:AO356_30360 Length = 245 Score = 93.2 bits (230), Expect = 6e-24 Identities = 65/185 (35%), Positives = 100/185 (54%), Gaps = 7/185 (3%) Query: 1 MDATQNEVLAEVRNHIGHLTLNRPAGLNAITLDMVRSLQQQLDAWAQDPQVHAVVLRGAG 60 +D +E+ E + +TLNR A NA+T+DM+ + L A DPQ++AV+L G+G Sbjct: 3 VDELASELTVERHESLLWITLNRAAKANAMTVDMMERMTAALQAATDDPQINAVMLTGSG 62 Query: 61 EKAFCAGGDIRSLYDSFKSGDTL--HEDFFVEEYALDLAIHHYRKPVLALMDGFVLGGGM 118 E+ FCAG DIR + GDT E + AL A+ KPV+ +++G GGG Sbjct: 63 ERVFCAGVDIR---EQPADGDTARQRERRSLALAALQDAVMDCPKPVVTVLNGTATGGGA 119 Query: 119 GLVQGADLRVVTERSRLAMPEVAIGYFPDVGGSHFLPRVPGE-LGIYLGVSGVQIRAADA 177 L AD V + ++L++PE+ +G G++ L + G L + L SG ++A +A Sbjct: 120 MLALLADACVAVDTAKLSLPEIDLG-IATFSGANILEVIGGRALALDLIQSGRAMKATEA 178 Query: 178 LYCGL 182 L GL Sbjct: 179 LARGL 183 Lambda K H 0.322 0.138 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 180 Number of extensions: 8 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 356 Length of database: 245 Length adjustment: 26 Effective length of query: 330 Effective length of database: 219 Effective search space: 72270 Effective search space used: 72270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory