GapMind for catabolism of small carbon sources

 

Protein Pf6N2E2_1455 in Pseudomonas fluorescens FW300-N2E2

Annotation: FitnessBrowser__pseudo6_N2E2:Pf6N2E2_1455

Length: 333 amino acids

Source: pseudo6_N2E2 in FitnessBrowser

Candidate for 9 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
D-xylose catabolism xylF hi D-xylose ABC transporter, periplasmic D-xylose-binding protein (characterized) 60% 100% 392.1 glucose transporter, periplasmic substrate-binding component 40% 228.4
D-cellobiose catabolism mglB med glucose transporter, periplasmic substrate-binding component (characterized) 40% 96% 228.4 D-xylose ABC transporter, periplasmic D-xylose-binding protein 60% 392.1
D-glucose catabolism mglB med glucose transporter, periplasmic substrate-binding component (characterized) 40% 96% 228.4 D-xylose ABC transporter, periplasmic D-xylose-binding protein 60% 392.1
lactose catabolism mglB med glucose transporter, periplasmic substrate-binding component (characterized) 40% 96% 228.4 D-xylose ABC transporter, periplasmic D-xylose-binding protein 60% 392.1
D-maltose catabolism mglB med glucose transporter, periplasmic substrate-binding component (characterized) 40% 96% 228.4 D-xylose ABC transporter, periplasmic D-xylose-binding protein 60% 392.1
sucrose catabolism mglB med glucose transporter, periplasmic substrate-binding component (characterized) 40% 96% 228.4 D-xylose ABC transporter, periplasmic D-xylose-binding protein 60% 392.1
trehalose catabolism mglB med glucose transporter, periplasmic substrate-binding component (characterized) 40% 96% 228.4 D-xylose ABC transporter, periplasmic D-xylose-binding protein 60% 392.1
L-arabinose catabolism chvE lo CVE1 aka ChvE aka ATU2348 aka AGR_C_4267, component of Multiple sugar (arabinose, xylose, galactose, glucose, fucose) putative porter (characterized) 32% 100% 166.8 D-xylose ABC transporter, periplasmic D-xylose-binding protein 60% 392.1
D-galactose catabolism chvE lo CVE1 aka ChvE aka ATU2348 aka AGR_C_4267, component of Multiple sugar (arabinose, xylose, galactose, glucose, fucose) putative porter (characterized) 32% 100% 166.8 D-xylose ABC transporter, periplasmic D-xylose-binding protein 60% 392.1

Sequence Analysis Tools

View Pf6N2E2_1455 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MKTFKRTLLAGALALLSLPVMADAAHPKIGFSIDDLRLERWSRDRDYFVAAAEKLDAKVF
VQSADANEQKQISQIENLISRGVDVIVIVPFNATVLTNAVAEAKKAGIKVVSYDRLILNA
DIDAYISFDNEKVGEMQASGVLKAAPKGNYFLLGGAPTDNNAKVLREGQMKVLQPAIDKG
DIKIVGQQWVKEWNPTEALSIVENALTRNNNKIDGIVASNDATAGGAIQALAAQKMAGKV
PISGQDADLAAVKRVIDGTQTMTVYKPLKLIASEAAKLSVQLARNEKPTFSSQYDNGSKK
VDTILLTPTPLTKDNIDLLEKDGFYTKAQIAGQ

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory