Protein Pf6N2E2_883 in Pseudomonas fluorescens FW300-N2E2
Annotation: FitnessBrowser__pseudo6_N2E2:Pf6N2E2_883
Length: 472 amino acids
Source: pseudo6_N2E2 in FitnessBrowser
Candidate for 21 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| myo-inositol catabolism | iolT | hi | Major myo-inositol transporter IolT (characterized) | 53% | 95% | 471.9 | Probable metabolite transport protein CsbC | 39% | 333.2 |
| D-cellobiose catabolism | MFS-glucose | med | Myo-Inositol uptake porter, IolT1 (Km=0.2mM) (characterized) | 48% | 96% | 430.3 | Major myo-inositol transporter IolT | 53% | 471.9 |
| D-glucose catabolism | MFS-glucose | med | Myo-Inositol uptake porter, IolT1 (Km=0.2mM) (characterized) | 48% | 96% | 430.3 | Major myo-inositol transporter IolT | 53% | 471.9 |
| lactose catabolism | MFS-glucose | med | Myo-Inositol uptake porter, IolT1 (Km=0.2mM) (characterized) | 48% | 96% | 430.3 | Major myo-inositol transporter IolT | 53% | 471.9 |
| D-maltose catabolism | MFS-glucose | med | Myo-Inositol uptake porter, IolT1 (Km=0.2mM) (characterized) | 48% | 96% | 430.3 | Major myo-inositol transporter IolT | 53% | 471.9 |
| sucrose catabolism | MFS-glucose | med | Myo-Inositol uptake porter, IolT1 (Km=0.2mM) (characterized) | 48% | 96% | 430.3 | Major myo-inositol transporter IolT | 53% | 471.9 |
| trehalose catabolism | MFS-glucose | med | Myo-Inositol uptake porter, IolT1 (Km=0.2mM) (characterized) | 48% | 96% | 430.3 | Major myo-inositol transporter IolT | 53% | 471.9 |
| D-xylose catabolism | xylT | lo | D-xylose transporter; D-xylose-proton symporter (characterized) | 36% | 95% | 306.2 | Major myo-inositol transporter IolT | 53% | 471.9 |
| L-arabinose catabolism | araE | lo | Arabinose-proton symporter; Arabinose transporter (characterized) | 34% | 99% | 283.5 | Major myo-inositol transporter IolT | 53% | 471.9 |
| D-galactose catabolism | galP | lo | Arabinose-proton symporter; Arabinose transporter (characterized) | 34% | 99% | 283.5 | Major myo-inositol transporter IolT | 53% | 471.9 |
| D-fructose catabolism | glcP | lo | D-fructose transporter, sugar porter family (characterized) | 34% | 93% | 245 | Major myo-inositol transporter IolT | 53% | 471.9 |
| sucrose catabolism | glcP | lo | D-fructose transporter, sugar porter family (characterized) | 34% | 93% | 245 | Major myo-inositol transporter IolT | 53% | 471.9 |
| glycerol catabolism | PLT5 | lo | polyol transporter 5 (characterized) | 31% | 85% | 241.1 | Major myo-inositol transporter IolT | 53% | 471.9 |
| D-mannitol catabolism | PLT5 | lo | polyol transporter 5 (characterized) | 31% | 85% | 241.1 | Major myo-inositol transporter IolT | 53% | 471.9 |
| D-ribose catabolism | PLT5 | lo | polyol transporter 5 (characterized) | 31% | 85% | 241.1 | Major myo-inositol transporter IolT | 53% | 471.9 |
| D-sorbitol (glucitol) catabolism | SOT | lo | polyol transporter 5 (characterized) | 31% | 85% | 241.1 | Major myo-inositol transporter IolT | 53% | 471.9 |
| xylitol catabolism | PLT5 | lo | polyol transporter 5 (characterized) | 31% | 85% | 241.1 | Major myo-inositol transporter IolT | 53% | 471.9 |
| D-fructose catabolism | Slc2a5 | lo | The fructose/xylose:H+ symporter, PMT1 (polyol monosaccharide transporter-1). Also transports other substrates at lower rates. PMT2 is largely of the same sequence and function. Both are present in pollen and young xylem cells (Klepek et al., 2005). A similar ortholog has been identifed in pollen grains of Petunia hybrida (characterized) | 31% | 96% | 236.1 | Major myo-inositol transporter IolT | 53% | 471.9 |
| sucrose catabolism | Slc2a5 | lo | The fructose/xylose:H+ symporter, PMT1 (polyol monosaccharide transporter-1). Also transports other substrates at lower rates. PMT2 is largely of the same sequence and function. Both are present in pollen and young xylem cells (Klepek et al., 2005). A similar ortholog has been identifed in pollen grains of Petunia hybrida (characterized) | 31% | 96% | 236.1 | Major myo-inositol transporter IolT | 53% | 471.9 |
| myo-inositol catabolism | HMIT | lo | Probable inositol transporter 2 (characterized) | 35% | 60% | 221.1 | Major myo-inositol transporter IolT | 53% | 471.9 |
| D-galacturonate catabolism | gatA | lo | The galacturonic acid (galacturonate) uptake porter, GatA, of 518 aas and 12 TMSs (characterized) | 30% | 91% | 194.5 | Major myo-inositol transporter IolT | 53% | 471.9 |
Sequence Analysis Tools
View Pf6N2E2_883 at FitnessBrowser
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
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Sequence
MTINSYGNTADTSAAYVSPEKHQAQRYLQKITWIATFGGLLFGFDTGVINGALLYMKDDL
GLTPFTEGLVASALLIGAMMGALFSGRLSDLKGRRRIILFLAVVFFLGALACALAPTLDV
MVAARFTLGLAVGGASVVVPAYLSEMAPSSIRGRIITRNELMIVTGQFLAFTTNATLGNL
FSDLDGVWRWMLALATLPAVALWLGMLYMPESPRWLATKGRFREGLEVLKLVREEYYAKA
EMEAITQQISNERFIKKGGWRDLSQKGARRIFLIGIGIAVTSQLTGVNSIMYFGTQILTE
AGLEQRSALIANVVNGIISIGATFVGIALLDRVGRRPMMLLGFTGTTLSLLLIGLVSVFV
DPSVTRAMLILGAMAMFLASMQGLIGPAFWVLLAEIFPMRIRGGCMGMAIAAFWLTNVMI
GMFFPSLVAMIGIGQTFFVFVGAGLLSLTFVAVWVPETRGSTLEEIEQRLYG
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory