Align Leucine/isoleucine/valine ABC transporter,ATPase component; EC 3.6.3.- (characterized, see rationale)
to candidate Pf6N2E2_1433 Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1)
Query= uniprot:G8ALJ0 (294 letters) >FitnessBrowser__pseudo6_N2E2:Pf6N2E2_1433 Length = 260 Score = 190 bits (483), Expect = 3e-53 Identities = 117/264 (44%), Positives = 161/264 (60%), Gaps = 17/264 (6%) Query: 9 TPLLTVEHLTMRFGGLVAVNDVSFSANNGEITAIIGPNGAGKTTLFNCITGFYTPTVGRL 68 T LL ++H+++ F G+ AV D+SF GEI A+IGPNGAGK++L N I+G Y G + Sbjct: 7 THLLELDHISLSFKGVKAVTDISFRVAAGEICALIGPNGAGKSSLLNVISGVYQAQDGHI 66 Query: 69 TLRHADGKEFLLERMPGYRISQKASVARTFQNIRLFGGMSVLENLIVAQHNKLIRASGFS 128 + + RM + ++ + +ARTFQNI LF GMSVL+N++ ++ K R++ Sbjct: 67 RFDRQERR-----RMRPHDVAVRG-IARTFQNIALFKGMSVLDNVLTGRNLKR-RSTWIE 119 Query: 129 IAGLLGLPSYTRTE----REAVDLAKYWLDRVRLLEFADWEAGNLPYGAQRRLEIARAMC 184 A +G RTE REA + +L L + D G LPYG Q+R+E+ARA+ Sbjct: 120 QALRIGR---ARTEDDRQREAAERVIAFL---HLQPWRDTLVGTLPYGLQKRVELARALA 173 Query: 185 TEPVMLCLDEPAAGLNPRESGELADLLTYIRDEHKIGVLLIEHDMSVVMTISDHVVVLDY 244 EP +L LDEP AG+N E +++ + I E V+LIEHD+ VVM ISDHVVVLDY Sbjct: 174 AEPRLLLLDEPMAGMNADEKQQMSRFIVDINRELGTTVVLIEHDIGVVMDISDHVVVLDY 233 Query: 245 GRKISDGDPAFVKNDPAVIRAYLG 268 GRKI DG P V+ +P VI AYLG Sbjct: 234 GRKIGDGSPEDVRQNPEVISAYLG 257 Lambda K H 0.319 0.137 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 185 Number of extensions: 8 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 294 Length of database: 260 Length adjustment: 25 Effective length of query: 269 Effective length of database: 235 Effective search space: 63215 Effective search space used: 63215 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory