Alignments for a candidate for glcD in Pseudomonas fluorescens FW300-N2E2

GapMind for catabolism of small carbon sources

Alignments for a candidate for glcD in Pseudomonas fluorescens FW300-N2E2

Align D-lactate oxidase and glycolate oxidase, FAD-linked subunit (EC 1.1.3.15) (characterized)
to candidate Pf6N2E2_137 Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD

Query= reanno::psRCH2:GFF3772
         (499 letters)



>FitnessBrowser__pseudo6_N2E2:Pf6N2E2_137
          Length = 511

 Score =  875 bits (2261), Expect = 0.0
 Identities = 428/499 (85%), Positives = 463/499 (92%)

Query: 1   MNILYDERVDGALPKVDKAALLAELQAQLPDLDILHRSEDLKPYECDGLSAYRTTPLLVV 60
           MNILYDE +DG LPKVDK ALL  LQAQ+PDLDILH+ EDLKPYECDGLSAYRTTPLLV 
Sbjct: 13  MNILYDEHLDGPLPKVDKQALLQALQAQIPDLDILHQEEDLKPYECDGLSAYRTTPLLVA 72

Query: 61  LPERIEQVETLLKLCHQRGVPVVARGAGTGLSGGALPLEQGILLVMARFNKILEVDPAGR 120
           LP R+EQV+ LLKLCH   VPVVARGAGTGLSGGALPLE G+LLVMARFN+IL +DP  R
Sbjct: 73  LPRRVEQVQALLKLCHGLDVPVVARGAGTGLSGGALPLESGVLLVMARFNQILHIDPDAR 132

Query: 121 FARVQPGVRNLAISQAAAPYELYYAPDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNLL 180
            AR+QPGVRNLAISQAAAP+ LYYAPDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNLL
Sbjct: 133 TARLQPGVRNLAISQAAAPFGLYYAPDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNLL 192

Query: 181 KVDILTVEGERMTLGSDALDSPGFDLLALFTGSEGMLGIVTEVTVKLLPKPQVAKVLLAA 240
           K++ILT+EGER+TLGS+ALDSPG DLLALFTGSEG+LG++TEVTVKLLP+PQVAKVLLA+
Sbjct: 193 KLEILTIEGERLTLGSEALDSPGLDLLALFTGSEGLLGVITEVTVKLLPRPQVAKVLLAS 252

Query: 241 FDSVEKAGRAVGDIIAAGIIPGGLEMMDNLSIRAAEDFIHAGYPVDAEAILLCELDGVEA 300
           FDSV+KAGRAV DIIAAGIIPGGLEMMDNL+IRAAEDFIHAGYPV+AEAILLCELDGVEA
Sbjct: 253 FDSVDKAGRAVADIIAAGIIPGGLEMMDNLAIRAAEDFIHAGYPVEAEAILLCELDGVEA 312

Query: 301 DVHDDCARVSEVLKLAGATEVRLAKDEAERVRFWAGRKNAFPAVGRISPDYYCMDGTIPR 360
           DVHDDC RV +VL+ AGATEVR A+DEAER+RFWAGRKNAFPAVGR++PDYYCMDGTIPR
Sbjct: 313 DVHDDCERVRQVLEQAGATEVRQARDEAERLRFWAGRKNAFPAVGRLAPDYYCMDGTIPR 372

Query: 361 RELPGVLKGISDLSEQFGLRVANVFHAGDGNMHPLILFDANQPGELERAEDLGGKILELC 420
           R LP VL+ I+ L  + GLRVANVFHAGDGNMHPLILFDANQPGELERAE LGGKILELC
Sbjct: 373 RALPEVLQRIASLGAEHGLRVANVFHAGDGNMHPLILFDANQPGELERAETLGGKILELC 432

Query: 421 VKVGGSITGEHGVGREKINQMCSQFNADELTLFHAVKAAFDPSGLLNPGKNIPTLHRCAE 480
           V+VGGSITGEHGVGREKINQMC+QFN+DEL LFHAVKAAFDP GLLNPGKNIPTLHRCAE
Sbjct: 433 VQVGGSITGEHGVGREKINQMCTQFNSDELNLFHAVKAAFDPQGLLNPGKNIPTLHRCAE 492

Query: 481 FGRMHIHNGQLPFPELERF 499
           FG MHIH GQLPFPELERF
Sbjct: 493 FGAMHIHGGQLPFPELERF 511


Lambda     K      H
   0.320    0.140    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 968
Number of extensions: 24
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 499
Length of database: 511
Length adjustment: 34
Effective length of query: 465
Effective length of database: 477
Effective search space:   221805
Effective search space used:   221805
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources