GapMind for catabolism of small carbon sources

 

Aligments for a candidate for artM in Pseudomonas fluorescens FW300-N2E2

Align ABC transporter for L-Arginine, permease component 1 (characterized)
to candidate Pf6N2E2_2960 Histidine ABC transporter, permease protein HisM (TC 3.A.1.3.1)

Query= reanno::BFirm:BPHYT_RS07680
         (237 letters)



>lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2960 Histidine ABC
           transporter, permease protein HisM (TC 3.A.1.3.1)
          Length = 236

 Score =  323 bits (828), Expect = 2e-93
 Identities = 155/232 (66%), Positives = 195/232 (84%)

Query: 1   MIDILNQFWRAFLYWDGQRMSGLAVTLWLLVASVGIGFCAAIPLAVARVSKKRWLSTPVR 60
           MI++L ++WR FLY DG  ++GLA+TLWLL AS+ IGF  +IPL++ARVS K ++  PV+
Sbjct: 1   MIELLQEYWRPFLYSDGVNVTGLAMTLWLLSASLLIGFLVSIPLSIARVSPKFYVRWPVQ 60

Query: 61  LYTYVFRGTPLYVQLLLIYTGMYSLEFVRSHQLLDAFFRSGFHCAILAFALNTCAYTTEI 120
            YTY+FRGTPLY+QLL+ YTG+YS+  VR+  LLD+FFR   +C ILAFALNTCAYTTEI
Sbjct: 61  FYTYLFRGTPLYIQLLICYTGIYSIAAVRAQPLLDSFFRDAMNCTILAFALNTCAYTTEI 120

Query: 121 FAGAIRSTSHGEVEAARAYGMSWFTMYRRIVIPSALRRALPLYSNEVILMLHATTVAFTA 180
           FAGAIRS +HGEVEAA+AYG++ + +Y  +++PSALRR+LP YSNEVILMLH+TTVAFTA
Sbjct: 121 FAGAIRSMNHGEVEAAKAYGLTGWRLYTYVIMPSALRRSLPYYSNEVILMLHSTTVAFTA 180

Query: 181 TVPDILKVARDANSATYQSFDAFGLAALIYLVVSFVLVALFRRAERHWLGYL 232
           TVPD+LKVARDANSAT+ +F +FG+AALIYL V+F LV LFR AER WL +L
Sbjct: 181 TVPDVLKVARDANSATFLTFQSFGIAALIYLTVTFALVGLFRLAERRWLAFL 232


Lambda     K      H
   0.331    0.139    0.437 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 251
Number of extensions: 10
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 237
Length of database: 236
Length adjustment: 23
Effective length of query: 214
Effective length of database: 213
Effective search space:    45582
Effective search space used:    45582
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (22.0 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory