Alignments for a candidate for kauB in Pseudomonas fluorescens FW300-N2E2

GapMind for catabolism of small carbon sources

Alignments for a candidate for kauB in Pseudomonas fluorescens FW300-N2E2

Align gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase (EC 1.2.1.54) (characterized)
to candidate Pf6N2E2_1309 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60)

Query= reanno::pseudo6_N2E2:Pf6N2E2_4383
         (497 letters)



>FitnessBrowser__pseudo6_N2E2:Pf6N2E2_1309
          Length = 486

 Score =  334 bits (857), Expect = 4e-96
 Identities = 188/474 (39%), Positives = 272/474 (57%), Gaps = 19/474 (4%)

Query: 33  SGETFDCLSPVDGRLLGKIASCDVADAQRAVENARATFSSGVWSRLAPSKRKATMIRFAG 92
           S + F   +P  G  +G++AS    +  +AV  A+  F    W+   P+K +A ++R  G
Sbjct: 13  SKDVFVNYNPATGEAIGEVASGGAEEVAQAVAAAKEAFPK--WAN-TPAKERARLMRKLG 69

Query: 93  -LLKQHAEELALLETLDMGKPISDSLNIDVPGAAQALSWSGEAIDKLYDEVAATPHDQLG 151
            L++Q+  +LA LETLD G PI  + N+ +P A+    +  E   ++           L 
Sbjct: 70  ELIEQNVPQLAELETLDTGLPIHQTKNVLIPRASHNFDFFAEVCTRMDGHTYPVDDQMLN 129

Query: 152 LVTREPVGVVGAIVPWNFPLMMACWKLGPALSTGNSVVLKPSEKSPLTALRIAALAIEAG 211
               +PVGV   + PWN P M A WK  P L+ GN+ VLK SE SPLTA  +  LA+EAG
Sbjct: 130 YTLYQPVGVCALVSPWNVPFMTATWKTAPCLALGNTAVLKMSELSPLTANELGRLAVEAG 189

Query: 212 IPKGVLNVLPGYGHTVGKALALHMDVDTLVFTGSTKIAKQLMIYSGESNMKRIWLEAGGK 271
           IP GVLNV+ GYG T G AL  H DV  + FTG T   K++M  +G   +K+  +E GGK
Sbjct: 190 IPNGVLNVIQGYGATAGDALVRHPDVRAISFTGGTATGKKIMQTAG---LKKYSMELGGK 246

Query: 272 SPNIVFADAPDLQAAAESAASAIAFNQGEVCTAGSRLLVERSIKDTFLPLVIEALKGWKP 331
           SP ++F DA DL+ A ++A   I    GE CTAGSR+ ++ S+   F+       K    
Sbjct: 247 SPVLIFEDA-DLERALDAALFTIFSLNGERCTAGSRIFIQESVYPQFVAEFAARAKRLIV 305

Query: 332 GNPLDPATNVGALVDTQQMNTVLSYIEAGHSDGAKLVAGGKRILEE--------TGGTYV 383
           G+P DP T VG+++     + V  YI+ G  +GA L+AGG   LE         + G ++
Sbjct: 306 GDPQDPKTQVGSMITQAHYDKVTGYIKIGLEEGATLLAGG---LERPANLPAHLSRGQFI 362

Query: 384 EPTIFDGVSNAMKIAQEEIFGPVLSVIAFDTAEQAIEIANDTPYGLAAAVWTKDISKAHL 443
           +PT+F  V+N M+IAQEEIFGPV+ +I F    +A+ +ANDT YGLA+ +WT+DI KAH 
Sbjct: 363 QPTVFADVNNKMRIAQEEIFGPVVCLIPFKDEAEALHLANDTEYGLASYIWTQDIGKAHR 422

Query: 444 TAKALRAGSVWVNQYDGGDMTAPFGGFKQSGNGRDKSLHAFDKYTELKSTWIKL 497
            A  + AG V++N  +  D+  PFGG K SG GR+   ++F+ + E+K+  I +
Sbjct: 423 LAHGIEAGMVFINSQNVRDLRQPFGGVKGSGTGREGGQYSFEVFAEIKNVCISM 476


Lambda     K      H
   0.316    0.132    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 589
Number of extensions: 24
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 497
Length of database: 486
Length adjustment: 34
Effective length of query: 463
Effective length of database: 452
Effective search space:   209276
Effective search space used:   209276
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources