Aligments for a candidate for glt in Pseudomonas fluorescens FW300-N2E2

GapMind for catabolism of small carbon sources

Aligments for a candidate for glt in Pseudomonas fluorescens FW300-N2E2

Align Amino acid:proton symporter (characterized, see rationale)
to candidate Pf6N2E2_2249 Proton/glutamate symport protein @ Sodium/glutamate symport protein

Query= uniprot:A0A0N9WTL5
         (431 letters)



>lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2249 Proton/glutamate
           symport protein @ Sodium/glutamate symport protein
          Length = 420

 Score =  790 bits (2039), Expect = 0.0
 Identities = 413/420 (98%), Positives = 415/420 (98%)

Query: 12  MGIALGVLVGWACHHFAGSEQSAKEIASYFSMVTDIFLRMIKMIIAPLVFATLVGGIASM 71
           MGIALGVLVGWACHHFAGSEQSAKEIASYFSMVTDIFLRMIKMIIAPLVFATLVGGIASM
Sbjct: 1   MGIALGVLVGWACHHFAGSEQSAKEIASYFSMVTDIFLRMIKMIIAPLVFATLVGGIASM 60

Query: 72  GNSRSVGRIGARAMAWFVTASVVSLLIGMGLVNLFQPGAGLNMDVAQHATAAVPVNTGDF 131
           GNSRSVGRIGARAMAWFVTASVVSLLIGMGLVNLFQPGAGLNMDVAQHATAAVPVNTGDF
Sbjct: 61  GNSRSVGRIGARAMAWFVTASVVSLLIGMGLVNLFQPGAGLNMDVAQHATAAVPVNTGDF 120

Query: 132 SLKAFIGHVFPRSIAEAMANNEILQIVVFSLFFGFALAGVKRAGYTRITDSIEELAKVMF 191
           SLKAFIGHVFPRSIAEAMANNEILQIVVFSLFFGFALAGVKRAGYTRITD IEELAKVMF
Sbjct: 121 SLKAFIGHVFPRSIAEAMANNEILQIVVFSLFFGFALAGVKRAGYTRITDCIEELAKVMF 180

Query: 192 KITDYVMAFAPIGVFAAIASAITTQGLGLLVDYGKLIAEFYLGILILWALLFGAGYLFLG 251
           KITDYVMAFAPIGVFAAIASAITTQGLGLLVDYGKLIAEFYLGILILWALLFGAGYLFLG
Sbjct: 181 KITDYVMAFAPIGVFAAIASAITTQGLGLLVDYGKLIAEFYLGILILWALLFGAGYLFLG 240

Query: 252 RSVFHLGKLIREPILLAFSTASSESAYPKTIEALEKFGAPKRVSSFVLPLGYSFNLDGSM 311
           RSVFHLGKLIREPILLAFSTASSESAYPKTIEALEKFGAPKRVSSFVLPLGYSFNLDGSM
Sbjct: 241 RSVFHLGKLIREPILLAFSTASSESAYPKTIEALEKFGAPKRVSSFVLPLGYSFNLDGSM 300

Query: 312 MYQAFAILFIAQAYNIDLSFTQQLLILLTLMITSKGMAGVARASVVVVAATLPMFNLPEA 371
           MYQAFAILFIAQAYNIDLSFTQQLLILLTLM+TSKGMAGVARASVVVVAATLPMFNLPEA
Sbjct: 301 MYQAFAILFIAQAYNIDLSFTQQLLILLTLMVTSKGMAGVARASVVVVAATLPMFNLPEA 360

Query: 372 GLLLIIGIDQFLDMARTATNVVGNSIATAVVAKSESHEEADEEEGEHAPARSRSEPVPVA 431
           GLLLIIGIDQFLDMARTATNVVGNSIATAVVAKSE HEEADE+EG  AP RSRSEPVPVA
Sbjct: 361 GLLLIIGIDQFLDMARTATNVVGNSIATAVVAKSEPHEEADEDEGAGAPVRSRSEPVPVA 420


Lambda     K      H
   0.325    0.138    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 690
Number of extensions: 16
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 431
Length of database: 420
Length adjustment: 32
Effective length of query: 399
Effective length of database: 388
Effective search space:   154812
Effective search space used:   154812
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources