GapMind for catabolism of small carbon sources

 

Alignments for a candidate for natF in Pseudomonas fluorescens FW300-N2E2

Align Extracellular solute-binding protein, family 3 (characterized, see rationale)
to candidate Pf6N2E2_5402 Glutamate Aspartate periplasmic binding protein precursor GltI (TC 3.A.1.3.4)

Query= uniprot:Q31RP1
         (359 letters)



>FitnessBrowser__pseudo6_N2E2:Pf6N2E2_5402
          Length = 343

 Score =  288 bits (737), Expect = 1e-82
 Identities = 146/345 (42%), Positives = 219/345 (63%), Gaps = 5/345 (1%)

Query: 17  RFLLVLLCFLPLAACRSLGGNETESNSRLNQVQARGKLLCGVEGRLPGFSFLDSQGNYSG 76
           + L   L  +  AA   + G   ++ + L+ VQ +G + CGV   LPGFS  DS G   G
Sbjct: 2   KLLKSTLAVMTAAAVLGVSGF-AQAGATLDAVQKKGFVQCGVSDGLPGFSVPDSTGKIVG 60

Query: 77  LDVDICKAIAAALFNDPKAIEYRSLDSVERFPALASGEVDLLSRNTTWTLSRDAKGGNNL 136
           +D D C+A+AAA+F D   +++  L++ ERF AL SGE+D+LSRN+T T SRDA  G  L
Sbjct: 61  IDADFCRAVAAAVFGDATKVKFSQLNAKERFTALQSGEIDMLSRNSTMTSSRDA--GMGL 118

Query: 137 EFAP-TTFYDGQGLMVRRNSGIQSLQDFQGKSICVETGTTSELNLADTMRELGVQYQEIK 195
           +F    T+YDG G +     G++S ++  G +IC++ GTT+ELN++D  R  G++Y  I 
Sbjct: 119 KFPGFITYYDGIGFLANNKLGVKSAKELDGATICIQAGTTTELNVSDYFRANGLKYTPIT 178

Query: 196 FPNSDANYAAYAQGRCEGVTSDRSQLAARRTTLSDADQHQLLDAVISKEPLSPATLNNDS 255
           F  SD +  +   GRC+ +TSD+SQL A+R+ L+    + +L   ISKEPL P   N D 
Sbjct: 179 FDTSDESAKSLESGRCDVLTSDKSQLFAQRSKLASPKDYVVLPETISKEPLGPVVRNGDD 238

Query: 256 PWFDVVKWVVNATIQAEEFGITQANID-QFKTSKNPEIRRFLGLEGELGQQLGLSNDFAY 314
            W  +V+W   A + AEE G+T  N++ + K++KNP++ R LG +GE G+ L L  D+  
Sbjct: 239 EWLAIVRWTGYALLNAEEAGVTSKNVEAEAKSTKNPDVARMLGADGEYGKDLKLPKDWVV 298

Query: 315 RAIKAVGNYGEIYERNVGQQSPLKLNRGLNQLYKNGGLLYSPPFR 359
           + +K VGNYGE++ERN+G+ +PL+++RGLN L+  GG+ Y+PP R
Sbjct: 299 QIVKQVGNYGEMFERNLGKGTPLEIDRGLNALWNAGGIQYAPPVR 343


Lambda     K      H
   0.317    0.134    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 286
Number of extensions: 10
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 359
Length of database: 343
Length adjustment: 29
Effective length of query: 330
Effective length of database: 314
Effective search space:   103620
Effective search space used:   103620
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory