Alignments for a candidate for astD in Pseudomonas fluorescens FW300-N2E2

GapMind for catabolism of small carbon sources

Alignments for a candidate for astD in Pseudomonas fluorescens FW300-N2E2

Align N-succinylglutamate 5-semialdehyde dehydrogenase; EC 1.2.1.71; Succinylglutamic semialdehyde dehydrogenase; SGSD (uncharacterized)
to candidate Pf6N2E2_368 Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16)

Query= curated2:Q1QTQ7
         (489 letters)



>FitnessBrowser__pseudo6_N2E2:Pf6N2E2_368
          Length = 485

 Score =  206 bits (523), Expect = 2e-57
 Identities = 157/463 (33%), Positives = 226/463 (48%), Gaps = 16/463 (3%)

Query: 9   IDGAWVDGD-AARFAKTDPVSGETLWTATAASATQVEHAVAAARQAFPDWARRSFAERQA 67
           +DG W+  D AA     DP +G +L    A    +   A+ AA +A+P W  R  AER A
Sbjct: 18  VDGQWIGADNAATLDVIDPATGHSLARVPAMQGVETRRAIEAAERAWPAWRARPAAERAA 77

Query: 68  VVERFRECLETHREHLATAIAQETGKPLWEARTEVGAMIGKVAISITAYHERTGER-ARD 126
           ++ER+ + +  + + LA  +  E GKPL EA+ E+    G             GE     
Sbjct: 78  LLERWYQAMMDNLDDLALIMTCEQGKPLSEAKGEIRYGAGFAKWFAEEARRVYGETIPAP 137

Query: 127 IGDARAVLRHRPHGVLAVYGPYNFPGHLPNGHIVPALLAGNAVVFKPSEQTPMTADLTLQ 186
            GD R +   +P GV A   P+NFP  +      PAL AG  V+ KPS+ TP++A     
Sbjct: 138 SGDRRLLTLKQPVGVCAAITPWNFPNAMITRKCAPALAAGCPVIVKPSDLTPLSALALAV 197

Query: 187 CWLEAGLPAGVINLVQG-AAEVGQALAGSADIDGLLFTGSAKVGGLLHRQFGGQVDKILA 245
                G+PAGV N+V G  A +G+ L G+  +  + FTGS  VG LL RQ    + K L+
Sbjct: 198 LAERVGIPAGVFNVVTGMPAGIGEELTGNPTVRKISFTGSTAVGRLLMRQSAEHI-KRLS 256

Query: 246 LELGGNNPLVVKDVPDREAAVLSILQSAFASGGQRCTCARRLIVPHGAVGDDLIDALTSA 305
           LELGGN P +V D  D E AV  I+ S F + GQ C CA R++V  G + +     L   
Sbjct: 257 LELGGNAPFIVFDDADLEQAVAGIMLSKFRNAGQTCVCANRILVQDG-IYERFAARLVEE 315

Query: 306 IAELRVAAPFSEPAPFYAGLTSVEAADGLLAAQDDLVARGGRPLSRMRRLQAGTSLLSPG 365
           + +L+V     E       L ++ A + +    DD +++G R L      +  +  + P 
Sbjct: 316 VGKLKVGNGL-EAGVMIGPLINLAAVNKVARHIDDALSQGARLLCG-GVPEGDSQFVQPT 373

Query: 366 LIDV--TGCDVPDEEHFGPLLKVHRYRDWDEAIALANDTRYGLSAGLIGGE-RADWDDFL 422
           ++     G  + +EE FGP+  + R+   ++A+ALAN T YGL A     + R  W  F 
Sbjct: 374 VLGEAHAGMLLANEETFGPVAPLMRFTTEEQALALANATPYGLGAYYFTQDLRRSW-RFG 432

Query: 423 LRIRAGIVNWNRQTTGASS--DAPFGGIGDSGNHRPSAYYAAD 463
             +  G+V  N   TG  S   APFGGI  SG  R  + Y  D
Sbjct: 433 EALEFGMVGLN---TGIISMEVAPFGGIKQSGLGREGSKYGLD 472


Lambda     K      H
   0.319    0.135    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 640
Number of extensions: 37
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 489
Length of database: 485
Length adjustment: 34
Effective length of query: 455
Effective length of database: 451
Effective search space:   205205
Effective search space used:   205205
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources