Align CVE1 aka ChvE aka ATU2348 aka AGR_C_4267, component of Multiple sugar (arabinose, xylose, galactose, glucose, fucose) putative porter (characterized)
to candidate Pf6N2E2_1455 Xylose ABC transporter, periplasmic xylose-binding protein XylF
Query= TCDB::P25548 (354 letters) >FitnessBrowser__pseudo6_N2E2:Pf6N2E2_1455 Length = 333 Score = 158 bits (400), Expect = 2e-43 Identities = 110/349 (31%), Positives = 179/349 (51%), Gaps = 22/349 (6%) Query: 1 MKSIISLMAACAIGAASFAAPAFAQDKGSVGIAMPTKSSARWIDDGNNIVKQLQEAGYKT 60 MK+ + A A+ S A A +G ++ RW D + V ++ K Sbjct: 1 MKTFKRTLLAGALALLSLPVMADAAHP-KIGFSIDDLRLERWSRDRDYFVAAAEKLDAKV 59 Query: 61 DLQYADDDIPNQLSQIENMVTKGVKVLVIASIDGTTLSDVLKQAGEQGIKVIAYDRLIRN 120 +Q AD + Q+SQIEN++++GV V+VI + T L++ + +A + GIKV++YDRLI N Sbjct: 60 FVQSADANEQKQISQIENLISRGVDVIVIVPFNATVLTNAVAEAKKAGIKVVSYDRLILN 119 Query: 121 SGDVSYYATFDNFQVGVLQATSITDKLGLKDGKGPFNIELFGGSPDDNNAFFFYDGAMSV 180 + D+ Y +FDN +VG +QA+ G+ N L GG+P DNNA +G M V Sbjct: 120 A-DIDAYISFDNEKVGEMQAS------GVLKAAPKGNYFLLGGAPTDNNAKVLREGQMKV 172 Query: 181 LKPYIDSGKLVVKSGQMGMDKVGTLRWDPATAQARMDNLLSAYYTDAKVDAVLSPYDGLS 240 L+P ID G + + Q W+P A + ++N L+ + K+D +++ D + Sbjct: 173 LQPAIDKGDIKIVGQQW------VKEWNPTEALSIVENALTR--NNNKIDGIVASNDATA 224 Query: 241 IGIISSLKGVGYGTKDQPLPVVSGQDAEVPSVKSIIAGEQYSTIFKDTRELAKVTVNMVN 300 G I +L K +P +SGQDA++ +VK +I G Q T++K + +A + Sbjct: 225 GGAIQALAAQKMAGK---VP-ISGQDADLAAVKRVIDGTQTMTVYKPLKLIASEAAKLSV 280 Query: 301 AVMEGKEPEVNDTKTYENGVKVVPSYLLKPVAVTKENYKQVLVDGGYYK 349 + ++P + Y+NG K V + LL P +TK+N + DG Y K Sbjct: 281 QLARNEKPTF--SSQYDNGSKKVDTILLTPTPLTKDNIDLLEKDGFYTK 327 Lambda K H 0.314 0.133 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 308 Number of extensions: 17 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 354 Length of database: 333 Length adjustment: 29 Effective length of query: 325 Effective length of database: 304 Effective search space: 98800 Effective search space used: 98800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory