GapMind for catabolism of small carbon sources

 

Alignments for a candidate for uxuL in Pseudomonas fluorescens FW300-N2E2

Align SMP-30/Gluconolaconase/LRE domain protein (characterized, see rationale)
to candidate Pf6N2E2_5966 L-arabinolactonase (EC 3.1.1.15)

Query= uniprot:B2UIY8
         (300 letters)



>FitnessBrowser__pseudo6_N2E2:Pf6N2E2_5966
          Length = 291

 Score =  131 bits (330), Expect = 2e-35
 Identities = 94/296 (31%), Positives = 138/296 (46%), Gaps = 45/296 (15%)

Query: 19  VGESPVWHAGEQALYWTDIPGRTLWRWNFFSGQTNNWPLPEMAGCIAMAPNGWAMA-MET 77
           +GE P W    QALYW DI G+   R          W +PE         +G A+  + +
Sbjct: 13  LGEGPFWDEPTQALYWVDIAGKQALR--LIGANVQIWQMPEHVSAFIPTQSGDALVTLSS 70

Query: 78  GIFLAPPPSPGIELGPLQRLATVAHARPDM--RFNDGRCDRQGRFWAGTM---VLDTSLG 132
           G++     SPG+E     RL  +  A P +  R N+ RCD  G+ W GTM   + + +  
Sbjct: 71  GVYRLDLDSPGLE----PRLTLLCMADPQLGNRANEARCDALGQLWLGTMQNNIGENAED 126

Query: 133 LPL----GKLYRLDAAAARTGRVDAVIDDLIVPNGLAFSPEGKTMYLSDSHASRQTVWAF 188
           LP+    G L+R+       GRV  ++  L +PN L +SP+G T+Y  +S          
Sbjct: 127 LPIERRSGGLFRVSG----DGRVMPLLRGLGIPNTLLWSPDGTTVYFGES---------- 172

Query: 189 DYDIDTGTPHNRRVFIDMNAYP----------GRPDGAAVDADGCYWICGNDAGFVHRFT 238
              +D GT +   +  D +  P          G PDG+A+DA G  W    D   + R  
Sbjct: 173 ---LD-GTLYRHFIHPDGSLAPAEVWFGPHPRGGPDGSAMDARGYIWNARWDGSCLLRLN 228

Query: 239 PDGRLDRSIAIPTSKPAMCAFGGPGLDTLFVTSIRIG-DDPLSGATFAVRPGVTGL 293
           P G++DR I +P S+P  C FGG  L TL++TS       PL GA  ++R  + G+
Sbjct: 229 PQGQVDRVIELPVSRPTSCVFGGDDLKTLYITSAASPLGHPLDGAVLSMRVDIPGV 284


Lambda     K      H
   0.322    0.140    0.466 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 377
Number of extensions: 25
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 300
Length of database: 291
Length adjustment: 26
Effective length of query: 274
Effective length of database: 265
Effective search space:    72610
Effective search space used:    72610
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory