Aligments for a candidate for MFS-glucose in Pseudomonas fluorescens FW300-N2E2

GapMind for catabolism of small carbon sources

Aligments for a candidate for MFS-glucose in Pseudomonas fluorescens FW300-N2E2

Align Myo-Inositol uptake porter, IolT1 (Km=0.2mM) (characterized)
to candidate Pf6N2E2_883 Major myo-inositol transporter IolT

Query= TCDB::Q8NTX0
         (491 letters)



>FitnessBrowser__pseudo6_N2E2:Pf6N2E2_883
          Length = 472

 Score =  427 bits (1097), Expect = e-124
 Identities = 219/442 (49%), Positives = 301/442 (68%), Gaps = 4/442 (0%)

Query: 32  VALVATFGGLLFGYDTGVINGALNPMTRELGLTAFTEGVVTSSLLFGAAAGAMFFGRISD 91
           +  +ATFGGLLFG+DTGVINGAL  M  +LGLT FTEG+V S+LL GA  GA+F GR+SD
Sbjct: 31  ITWIATFGGLLFGFDTGVINGALLYMKDDLGLTPFTEGLVASALLIGAMMGALFSGRLSD 90

Query: 92  NWGRRKTIISLAVAFFVGTMICVFAPSFAVMVVGRVLLGLAVGGASTVVPVYLAELAPFE 151
             GRR+ I+ LAV FF+G + C  AP+  VMV  R  LGLAVGGAS VVP YL+E+AP  
Sbjct: 91  LKGRRRIILFLAVVFFLGALACALAPTLDVMVAARFTLGLAVGGASVVVPAYLSEMAPSS 150

Query: 152 IRGSLAGRNELMIVVGQLAAFVINAIIGNVFGHHDGVWRYMLAIAAIPAIALFFGMLRVP 211
           IRG +  RNELMIV GQ  AF  NA +GN+F   DGVWR+MLA+A +PA+AL+ GML +P
Sbjct: 151 IRGRIITRNELMIVTGQFLAFTTNATLGNLFSDLDGVWRWMLALATLPAVALWLGMLYMP 210

Query: 212 ESPRWLVERGRIDEARAVLETIRPLERAHAEVADVEHLAREEHAVSEKSMGLREILSSKW 271
           ESPRWL  +GR  E   VL+ +R    A AE+  +      E  +  K  G R+ LS K 
Sbjct: 211 ESPRWLATKGRFREGLEVLKLVREEYYAKAEMEAITQQISNERFI--KKGGWRD-LSQKG 267

Query: 272 LVRILLVGIGLGVAQQLTGINSIMYYGQVVLIEAGFSENAALIANVAPGVIAVVGAFIAL 331
             RI L+GIG+ V  QLTG+NSIMY+G  +L EAG  + +ALIANV  G+I++   F+ +
Sbjct: 268 ARRIFLIGIGIAVTSQLTGVNSIMYFGTQILTEAGLEQRSALIANVVNGIISIGATFVGI 327

Query: 332 WMMDRINRRTTLITGYSLTTISHVLIGIASVAFPVGDPLRPYVILTLVVVFVGSMQTFLN 391
            ++DR+ RR  ++ G++ TT+S +LIG+ SV F      R  +IL  + +F+ SMQ  + 
Sbjct: 328 ALLDRVGRRPMMLLGFTGTTLSLLLIGLVSV-FVDPSVTRAMLILGAMAMFLASMQGLIG 386

Query: 392 VATWVMLSELFPLAMRGFAIGISVFFLWIANAFLGLFFPTIMEAVGLTGTFFMFAGIGVV 451
            A WV+L+E+FP+ +RG  +G+++   W+ N  +G+FFP+++  +G+  TFF+F G G++
Sbjct: 387 PAFWVLLAEIFPMRIRGGCMGMAIAAFWLTNVMIGMFFPSLVAMIGIGQTFFVFVGAGLL 446

Query: 452 ALIFIYTQVPETRGRTLEEIDE 473
           +L F+   VPETRG TLEEI++
Sbjct: 447 SLTFVAVWVPETRGSTLEEIEQ 468


Lambda     K      H
   0.327    0.142    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 623
Number of extensions: 20
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 491
Length of database: 472
Length adjustment: 34
Effective length of query: 457
Effective length of database: 438
Effective search space:   200166
Effective search space used:   200166
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources