Align Sodium/glutamate symporter; Glutamate permease (characterized)
to candidate Pf6N2E2_4368 Sodium/glutamate symport protein
Query= SwissProt::P0AER8 (401 letters) >FitnessBrowser__pseudo6_N2E2:Pf6N2E2_4368 Length = 402 Score = 537 bits (1383), Expect = e-157 Identities = 277/401 (69%), Positives = 330/401 (82%), Gaps = 2/401 (0%) Query: 1 MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLLVALALLVLKKSMGWEV 60 MF LD TLVAA+L LLLGR LV V FL+ Y IPEPVAGGL+VAL LL L+ + EV Sbjct: 1 MFQLDFYGTLVAASLVLLLGRGLVTRVGFLRAYNIPEPVAGGLVVALVLLALR-GLEVEV 59 Query: 61 NFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVVGLLVMQNAIGIGMASLLG 120 FD SL+ PLMLAFFATIGL+A+ ASL+ GGRVVGIFL+ VVGLLV+QNA+G+G+A++LG Sbjct: 60 RFDTSLQTPLMLAFFATIGLSADFASLKKGGRVVGIFLLAVVGLLVVQNAMGVGLATVLG 119 Query: 121 LDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEVAMACATFGLVLGGLIGGPVA 180 LDPLMGLL GSITL+GGHGTGAAW +F E++G +A+E+AMA ATFGLVLGGLIGGPVA Sbjct: 120 LDPLMGLLTGSITLAGGHGTGAAWGTVFSEKFGLASASELAMASATFGLVLGGLIGGPVA 179 Query: 181 RYLVKHSTTPNGIPDDQ-EVPTAFEKPDVGRMITSLVLIETIALIAICLTVGKIVAQLLA 239 R L+K +P G+ ++ +P FE+P+ R IT IET+ALIA+ L G ++ L Sbjct: 180 RLLIKRVQSPGGLESEKPRIPKGFEQPNKERSITPFSFIETLALIAVSLLAGTLLNGQLH 239 Query: 240 GTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVSVLGNVSLSLFLAMALMGLKLWEL 299 TAFELPTFVCVLFVGV+L NGLS +G Y+VFER VSVLGNVSLSLFLA+ALM LKLW+L Sbjct: 240 DTAFELPTFVCVLFVGVVLRNGLSALGLYQVFEREVSVLGNVSLSLFLAIALMSLKLWDL 299 Query: 300 ASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYDAAVLAAGHCGFGLGATPTAIANMQA 359 ASLALP+ IL QT+ MAL+AIFVT+R+MG +YDAAVLAAGHCGFGLGATPTAIANMQA Sbjct: 300 ASLALPIFIILAAQTLVMALFAIFVTFRIMGSHYDAAVLAAGHCGFGLGATPTAIANMQA 359 Query: 360 ITERFGPSHMAFLVVPMVGAFFIDIVNALVIKLYLMLPIFA 400 +T+R+GPS +AFLVVPMVGAFFIDI+N +VIKLYL LP+FA Sbjct: 360 VTQRYGPSQIAFLVVPMVGAFFIDIINVIVIKLYLALPLFA 400 Lambda K H 0.329 0.143 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 462 Number of extensions: 15 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 401 Length of database: 402 Length adjustment: 31 Effective length of query: 370 Effective length of database: 371 Effective search space: 137270 Effective search space used: 137270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory