GapMind for catabolism of small carbon sources

 

Alignments for a candidate for hutX in Pseudomonas fluorescens FW300-N2E2

Align ABC transporter for L-Histidine, periplasmic substrate-binding component (characterized)
to candidate Pf6N2E2_3803 Histidine ABC transporter, histidine-binding protein (TC 3.A.1)

Query= reanno::pseudo5_N2C3_1:AO356_09620
         (322 letters)



>FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3803
          Length = 322

 Score =  647 bits (1669), Expect = 0.0
 Identities = 319/322 (99%), Positives = 319/322 (99%)

Query: 1   MKSNKTLLTTLLSMGLLASAGATQAAGWCESGKPVKFAGLNWESGMLLTDVLQVVLEKGY 60
           MKSNKTLLTTLLSMGLLA AGATQAAGWCESGKPVKFAGLNWESGMLLTDVLQVVLEKGY
Sbjct: 1   MKSNKTLLTTLLSMGLLAGAGATQAAGWCESGKPVKFAGLNWESGMLLTDVLQVVLEKGY 60

Query: 61  DCKTDSLPGNSITMENALSSNDIQVFAEEWVGRSEVWNKAEKAGKVVGVGAPVVGAIEGW 120
            CKTDSLPGNSITMENALSSNDIQVFAEEWVGRSEVWNKAEKAGKVVGVGAPVVGAIEGW
Sbjct: 61  GCKTDSLPGNSITMENALSSNDIQVFAEEWVGRSEVWNKAEKAGKVVGVGAPVVGAIEGW 120

Query: 121 YVPRYVVEGDAKRKLEAKAPGLKNIADLGQYAAVFKDPEEPSKGRFYNCPAGWTCELDNS 180
           YVPRYVVEGDAKRKLEAKAPGLKNIADLGQYAAVFKDPEEPSKGRFYNCPAGWTCELDNS
Sbjct: 121 YVPRYVVEGDAKRKLEAKAPGLKNIADLGQYAAVFKDPEEPSKGRFYNCPAGWTCELDNS 180

Query: 181 EMLKSYGLEKTYTNFRPGTGPALDAAVLSSYKRGEPILFYYWSPTPLMGQVDLVKLEEKP 240
           EMLKSYGLEKTYTNFRPGTGPALDAAVLSSYKRGEPILFYYWSPTPLMGQVDLVKLEEKP
Sbjct: 181 EMLKSYGLEKTYTNFRPGTGPALDAAVLSSYKRGEPILFYYWSPTPLMGQVDLVKLEEKP 240

Query: 241 GVDKSVSIKVGLSKTFHDEAPELVAVLEKVNLPIDILNQNLGRMAKERIESPKLAKIFLK 300
           GVDKSVSIKVGLSKTFHDEAPELV VLEKVNLPIDILNQNLGRMAKERIESPKLAKIFLK
Sbjct: 241 GVDKSVSIKVGLSKTFHDEAPELVTVLEKVNLPIDILNQNLGRMAKERIESPKLAKIFLK 300

Query: 301 EHPEVWHAWVSEDAAKKIDAAL 322
           EHPEVWHAWVSEDAAKKIDAAL
Sbjct: 301 EHPEVWHAWVSEDAAKKIDAAL 322


Lambda     K      H
   0.314    0.133    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 494
Number of extensions: 24
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 322
Length of database: 322
Length adjustment: 28
Effective length of query: 294
Effective length of database: 294
Effective search space:    86436
Effective search space used:    86436
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory