GapMind for catabolism of small carbon sources

 

Finding step livG for L-isoleucine catabolism in Pseudomonas fluorescens FW300-N2E2

4 candidates for livG: L-isoleucine ABC transporter, ATPase component 2 (LivG/BraF)

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other bits
hi Pf6N2E2_2925 Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1) High-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG (characterized, see rationale) 100% 100% 508.4 Putative branched-chain amino acid transport system ATP-binding protein, component of The phenylpropeneoid uptake porter, CouPSTW 44% 209.1
hi Pf6N2E2_3577 Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1) ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale) 48% 91% 275 Putative branched-chain amino acid transport system ATP-binding protein, component of The phenylpropeneoid uptake porter, CouPSTW 43% 206.1
hi Pf6N2E2_1705 Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1) ABC transporter ATP-binding protein (characterized, see rationale) 41% 97% 198 Putative branched-chain amino acid transport system ATP-binding protein, component of The phenylpropeneoid uptake porter, CouPSTW 41% 184.5
med Pf6N2E2_1433 Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1) ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale) 42% 89% 202.6 Putative branched-chain amino acid transport system ATP-binding protein, component of The phenylpropeneoid uptake porter, CouPSTW 44% 198.7

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

Also see fitness data for the candidates

Definition of step livG

Or cluster all characterized livG proteins

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory