GapMind for catabolism of small carbon sources

 

Finding step livH for L-isoleucine catabolism in Pseudomonas fluorescens FW300-N2E2

4 candidates for livH: L-isoleucine ABC transporter, permease component 1 (LivH/BraD)

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other bits
hi Pf6N2E2_2923 High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1) Branched-chain amino acid ABC transporter permease LivH; SubName: Full=Branched-chain amino acid transporter permease subunit LivH; SubName: Full=L-leucine ABC transporter membrane protein /L-isoleucine ABC transporter membrane protein /L-valine ABC transporter membrane protein (characterized, see rationale) 100% 100% 588.6 L-proline and D-alanine ABC transporter, permease component 1 57% 351.7
med Pf6N2E2_3579 High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1) Branched-chain amino acid ABC transporter permease LivH; SubName: Full=Branched-chain amino acid transporter permease subunit LivH; SubName: Full=L-leucine ABC transporter membrane protein /L-isoleucine ABC transporter membrane protein /L-valine ABC transporter membrane protein (characterized, see rationale) 55% 98% 336.3 L-proline and D-alanine ABC transporter, permease component 1 58% 351.7
med Pf6N2E2_1704 High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1) Transmembrane component of a broad range amino acid ABC transporter (characterized, see rationale) 31% 100% 134 ABC amino acid transporter, permease component, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog 31% 119.8
lo Pf6N2E2_3548 Urea ABC transporter, permease protein UrtB ABC transporter permease (characterized, see rationale) 30% 98% 118.6 UrtA, component of The high affinity urea/thiourea/hydroxyurea porter 46% 260.4

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

Also see fitness data for the candidates

Definition of step livH

Or cluster all characterized livH proteins

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory