Alignments for a candidate for glk in Pseudomonas fluorescens FW300-N2E2

GapMind for catabolism of small carbon sources

Alignments for a candidate for glk in Pseudomonas fluorescens FW300-N2E2

Align Glucokinase (EC 2.7.1.2) (characterized)
to candidate Pf6N2E2_2897 Glucokinase (EC 2.7.1.2)

Query= reanno::WCS417:GFF4431
         (318 letters)



>FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2897
          Length = 319

 Score =  552 bits (1422), Expect = e-162
 Identities = 267/318 (83%), Positives = 293/318 (92%)

Query: 1   MKLALVGDIGGTNARFALWRDQELHSIRVHATADHSSPEDAIKVYLKEEGLEIGDIGAVC 60
           +KLALVGDIGGTNARFALW++  L +I+V ATAD++ PEDAI+VYL   GL+ G IG+VC
Sbjct: 1   LKLALVGDIGGTNARFALWKNHTLENIQVLATADYACPEDAIQVYLSGLGLKQGAIGSVC 60

Query: 61  LSVAGPVSGDEFKFTNNHWRLSKTAFCKTLQVDELLLVNDFSAMALGMTRLKPDEFRVVC 120
           LSVAGPVSGDEF+FTNNHWRLS  AFC+TLQV++LLLVNDFSAMALGMT L+PDE+RVVC
Sbjct: 61  LSVAGPVSGDEFRFTNNHWRLSNLAFCQTLQVEKLLLVNDFSAMALGMTCLRPDEYRVVC 120

Query: 121 EGTPEPLRPAVVIGPGTGLGVGTLLDLGAGRFAALPGEGGHVDLPLSSPRETQLWQHIYT 180
           EGTPEPLRPAVVIGPGTGLGVGTLLDLG GRFAALPGEGGHVDLPLSSPRETQLWQHIY 
Sbjct: 121 EGTPEPLRPAVVIGPGTGLGVGTLLDLGEGRFAALPGEGGHVDLPLSSPRETQLWQHIYN 180

Query: 181 EIGHVSAETALSGGGLPRLYRAICAVDGHTPVLETPEAITAAGLAGDPVAMEVLDQFSIW 240
           EIGHVSAETALSG GLPR+YRAICAVDGH PVL+TPE+ITAAGLAGDP+A+EVL+QF  W
Sbjct: 181 EIGHVSAETALSGSGLPRVYRAICAVDGHVPVLDTPESITAAGLAGDPIALEVLEQFCRW 240

Query: 241 LGRVAGNNVLTTGGRGGVYIVGGVIPRFADFFINSGFAKSFADKGCMSDYFKGIPVWLVT 300
           LGRVAGNNVLT GGRGGVYIVGGV+PRFADFF+ SGFA+ FADKGCMSDYFKGIPVWLVT
Sbjct: 241 LGRVAGNNVLTLGGRGGVYIVGGVVPRFADFFLESGFARCFADKGCMSDYFKGIPVWLVT 300

Query: 301 APYSGLTGAGVALEQAFA 318
           APY GL GAGVALEQ+ A
Sbjct: 301 APYPGLMGAGVALEQSAA 318


Lambda     K      H
   0.320    0.139    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 462
Number of extensions: 10
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 318
Length of database: 319
Length adjustment: 27
Effective length of query: 291
Effective length of database: 292
Effective search space:    84972
Effective search space used:    84972
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 48 (23.1 bits)

Align candidate Pf6N2E2_2897 (Glucokinase (EC 2.7.1.2))
to HMM TIGR00749 (glk: glucokinase (EC 2.7.1.2))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR00749.hmm
# target sequence database:        /tmp/gapView.32675.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00749  [M=315]
Accession:   TIGR00749
Description: glk: glucokinase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                      Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                      -----------
    2.4e-83  266.0   0.0    2.8e-83  265.8   0.0    1.0  1  lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2897  Glucokinase (EC 2.7.1.2)


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2897  Glucokinase (EC 2.7.1.2)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  265.8   0.0   2.8e-83   2.8e-83       1     315 []       5     310 ..       5     310 .. 0.96

  Alignments for each domain:
  == domain 1  score: 265.8 bits;  conditional E-value: 2.8e-83
                                      TIGR00749   1 lvgdiGGtnarlalvevapgeieqvktyssedfpsleavvrvyleeakvelkdpi.kgcfaiat 63 
                                                    lvgdiGGtnar+al       +e+++++ + d+   e++++vyl+    + +  i   c+++a+
  lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2897   5 LVGDIGGTNARFAL--WKNHTLENIQVLATADYACPEDAIQVYLSGLGLKQGA-IgSVCLSVAG 65 
                                                    89************..78899************************98877777.5599****** PP

                                      TIGR00749  64 PiigdfvrltnldWalsieelkqelalaklelindfaavayailalkeedliqlggakveesaa 127
                                                    P+ gd  r+tn++W ls     q+l ++kl l+ndf+a+a++++ l+ ++  ++    +e+  +
  lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2897  66 PVSGDEFRFTNNHWRLSNLAFCQTLQVEKLLLVNDFSAMALGMTCLRPDEYRVVCEGTPEPLRP 129
                                                    **************************************************************** PP

                                      TIGR00749 128 iailGaGtGlGvatliqqsdgrykvlageGghvdfaPrseleillleylrkkygrvsaervlsG 191
                                                      ++G+GtGlGv tl++ ++gr+ +l+geGghvd+   s+ e++l++ + +++g+vsae  lsG
  lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2897 130 AVVIGPGTGLGVGTLLDLGEGRFAALPGEGGHVDLPLSSPRETQLWQHIYNEIGHVSAETALSG 193
                                                    **************************************************************** PP

                                      TIGR00749 192 sGlvliyealskrkgerevsklskeelkekdiseaalegsdvlarralelflsilGalagnlal 255
                                                    sGl  +y+a+   +g  +v  l     +++ i+ a l+g+ ++a + le f+  lG++agn  l
  lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2897 194 SGLPRVYRAICAVDG--HVPVLD----TPESITAAGLAGD-PIALEVLEQFCRWLGRVAGNNVL 250
                                                    ************994..344455....99*********96.89999****************** PP

                                      TIGR00749 256 klgarGGvyvaGGivPrfiellkkssfraafedkGrlkellasiPvqvvlkkkvGllGag 315
                                                    +lg rGGvy++GG+vPrf +++ +s+f   f dkG + ++++ iPv +v    +Gl+Gag
  lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2897 251 TLGGRGGVYIVGGVVPRFADFFLESGFARCFADKGCMSDYFKGIPVWLVTAPYPGLMGAG 310
                                                    **********************************************************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (315 nodes)
Target sequences:                          1  (319 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.00
# Mc/sec: 11.28
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources