GapMind for catabolism of small carbon sources

 

Alignments for a candidate for aapM in Pseudomonas fluorescens FW300-N2E2

Align AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized)
to candidate Pf6N2E2_5568 Glutamate Aspartate transport system permease protein GltK (TC 3.A.1.3.4)

Query= TCDB::Q52814
         (384 letters)



>FitnessBrowser__pseudo6_N2E2:Pf6N2E2_5568
          Length = 223

 Score =  107 bits (268), Expect = 2e-28
 Identities = 65/203 (32%), Positives = 107/203 (52%), Gaps = 9/203 (4%)

Query: 171 LWGGLMVTLVLSFVGIAVSLPVGILLALGRRSRMPVIRMLCVTFIEVIRGVPLITVLFMA 230
           LW G+++TL L  +G+   + +G +LAL R S   ++  +   ++   R +PL+ V+   
Sbjct: 15  LWNGMVMTLKLMALGVVGGIILGTILALMRLSHNKLVSNIAGAYVNYFRSIPLLLVITWF 74

Query: 231 SVMLPLFLPTGWNVDKLLRA----LIGVSIFTSAYMAEVIRGGLQAIPKGQFEGADSLGL 286
            + +P  L      D  + A    ++   +F +AY  E++R G+Q+IPKGQ   A +LG+
Sbjct: 75  YLAVPFVLRWITGEDTPIGAFGSCIVAFMMFEAAYFCEIVRAGVQSIPKGQMGAAQALGM 134

Query: 287 GYWQKTRLIIMPQAIKLVIPSIVNTFIGTFKDTSLVTIIGMFDLLGIVKLNFSDANWASA 346
            Y Q  RLII+PQA + + P ++   I  F+DTSLV  +G+ D      LN S A+    
Sbjct: 135 NYGQTMRLIILPQAFRKMTPLLLQQSIILFQDTSLVYTVGLVDF-----LNASRASGDII 189

Query: 347 VTPITGLIFAGFIFWLFCFGMSR 369
                 LI AG +++   F  S+
Sbjct: 190 GRSNEFLIIAGLVYFTVSFAASQ 212


Lambda     K      H
   0.330    0.145    0.469 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 246
Number of extensions: 13
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 384
Length of database: 223
Length adjustment: 26
Effective length of query: 358
Effective length of database: 197
Effective search space:    70526
Effective search space used:    70526
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory