Alignments for a candidate for amaB in Pseudomonas fluorescens FW300-N2E2

GapMind for catabolism of small carbon sources

Alignments for a candidate for amaB in Pseudomonas fluorescens FW300-N2E2

Align L-piperidine-6-carboxylate dehydrogenase; EC 1.2.1.21 (characterized, see rationale)
to candidate Pf6N2E2_3582 L-pipecolate dehydrogenase (EC 1.5.99.3)

Query= uniprot:Q88CC3
         (496 letters)



>FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3582
          Length = 496

 Score =  806 bits (2081), Expect = 0.0
 Identities = 399/496 (80%), Positives = 437/496 (88%)

Query: 1   MVAGLLERLGVAAEAYTQGDYPVHTPIDGSQIASVKLLGKAETIARIDQAQSAFEAWRSV 60
           MVA LL+RLGV +  Y  G  PVH+PIDGS+I +V   G AE   ++ +A+ AF+ WR V
Sbjct: 1   MVAALLDRLGVDSALYQNGTQPVHSPIDGSRIGAVNWEGAAEVEQQVSRAEHAFDLWRQV 60

Query: 61  PAPRRGELVRLFGEVLREHKADLGELVSIEAGKITQEGLGEVQEMIDICDFAVGLSRQLY 120
           PAPRRGELVR FG++LRE+KADLGELVS EAGKITQEGLGEVQEMIDICDFAVGLSRQLY
Sbjct: 61  PAPRRGELVRQFGDLLREYKADLGELVSWEAGKITQEGLGEVQEMIDICDFAVGLSRQLY 120

Query: 121 GLTIASERPGHHMRETWHPLGVVGVISAFNFPVAVWAWNTALALVAGNSVVWKPSEKTPL 180
           GLTIASERPGHHMRE+WHPLGVVGVISAFNFPVAVWAWNT LALV GN+V+WKPSEKTPL
Sbjct: 121 GLTIASERPGHHMRESWHPLGVVGVISAFNFPVAVWAWNTTLALVCGNAVIWKPSEKTPL 180

Query: 181 TALACQALFEKALKAFGDAPAGLAQLVIGGREAGEAMVDDPRVPLVSATGSTRMGREVGP 240
           TALACQALFE+ LK F DAP  L+Q++IGGR+AGEA+VDDPRV L+SATGSTRMGREV P
Sbjct: 181 TALACQALFERVLKNFSDAPPYLSQVIIGGRDAGEALVDDPRVALISATGSTRMGREVAP 240

Query: 241 RVAARFGRSILELGGNNAMILAPSADLDLAVRGILFSAVGTAGQRCTTLRRLIVHRSIKD 300
           +VAARF RSILELGGNNAMILAPSADLD+AVR ILFSAVGTAGQRCTTLRRLI H S+K+
Sbjct: 241 KVAARFARSILELGGNNAMILAPSADLDMAVRAILFSAVGTAGQRCTTLRRLIAHESVKE 300

Query: 301 EVVARVKAAYGKVRIGDPRKDNLVGPLIDKQSFDAMQGALAKARDEGGQVFGGERQLADQ 360
           E+V R+KAAY KVRIG P + NLVGPLIDK SFD MQ AL +A  EGG+VFGG+RQL DQ
Sbjct: 301 EIVTRLKAAYSKVRIGHPLEGNLVGPLIDKHSFDNMQDALEQALSEGGRVFGGKRQLEDQ 360

Query: 361 YPNAYYVSPAIAEMPAQSDVVRHETFAPILYVLAYDDFEEALRLNNEVPQGLSSCIFTTD 420
           +PNAYYVSPAI EMP QSDVVRHETFAPILYV+ Y DF EAL LNN VPQGLSSCIFTTD
Sbjct: 361 FPNAYYVSPAIVEMPEQSDVVRHETFAPILYVVGYKDFAEALHLNNAVPQGLSSCIFTTD 420

Query: 421 IREAERFQSASGSDCGIANVNIGTSGAEIGGAFGGEKETGGGRESGSDAWKGYMRRQTNT 480
           +REAE+F SA GSDCGIANVNIG SGAEIGGAFGGEKETGGGRESGSDAW+GYMRRQTNT
Sbjct: 421 VREAEQFMSAVGSDCGIANVNIGPSGAEIGGAFGGEKETGGGRESGSDAWRGYMRRQTNT 480

Query: 481 VNYSRELPLAQGIVFD 496
           VNYS ELPLAQGI FD
Sbjct: 481 VNYSLELPLAQGITFD 496


Lambda     K      H
   0.318    0.135    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 836
Number of extensions: 20
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 496
Length of database: 496
Length adjustment: 34
Effective length of query: 462
Effective length of database: 462
Effective search space:   213444
Effective search space used:   213444
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources