Align lysine/arginine/ornithine ABC transporter, periplasmic lysine/arginine/ornithine-binding protein ArgT (characterized)
to candidate Pf6N2E2_1098 Arginine/ornithine ABC transporter, periplasmic arginine/ornithine binding protein
Query= CharProtDB::CH_003045 (260 letters) >FitnessBrowser__pseudo6_N2E2:Pf6N2E2_1098 Length = 260 Score = 222 bits (565), Expect = 7e-63 Identities = 106/256 (41%), Positives = 161/256 (62%), Gaps = 2/256 (0%) Query: 6 LALSL-LIGLGATAASYAALPQTVRIGTDTTYAPFSSKDAKGEFIGFDIDLGNEMCKRMQ 64 +AL L L+ L A S A ++RIG + Y PF+SK +GFD D+G +C M+ Sbjct: 6 IALKLGLVSLLAFGTSLQAAESSLRIGIEAAYPPFASKTPDNAIVGFDYDIGQALCAEMK 65 Query: 65 VKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQEIAFSDKLYAADSRLIAAKGSPI 124 V+C W +FD LIP+LK KK+DA+ISS+SIT +R + + F+D+ Y +RL+ KGS I Sbjct: 66 VRCVWQEQEFDGLIPALKVKKVDAVISSMSITPERLKSVDFTDRYYRIPARLVFRKGSGI 125 Query: 125 QPTLESLKGKHVGVLQGSTQEAYANDNWRTKGVDVVAYANQDLIYSDLTAGRLDAALQDE 184 L GK +GV + + + Y D++ G +VV Y +Q+ I+ DL GRLDA + Sbjct: 126 NDIPAQLNGKRIGVQRATNFDRYVTDHFAPAGAEVVRYGSQNEIFLDLLGGRLDATMASS 185 Query: 185 VAASEGFLKQPAGKEYAFAGPSVKDKKYFGDGTGVGLRKDDTELKAAFDKALTELRQDGT 244 V E LK+P G ++ F GP+ +++YFG G G+ +RK+DT L F++AL +R +GT Sbjct: 186 VVIDESLLKRPEGNDFEFVGPNFTEEQYFGTGIGIAVRKNDT-LAGRFNQALATIRANGT 244 Query: 245 YDKMAKKYFDFNVYGD 260 Y+++ +KYFDF++YG+ Sbjct: 245 YERIRQKYFDFDIYGE 260 Lambda K H 0.315 0.132 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 190 Number of extensions: 8 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 260 Length of database: 260 Length adjustment: 24 Effective length of query: 236 Effective length of database: 236 Effective search space: 55696 Effective search space used: 55696 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory