GapMind for catabolism of small carbon sources

 

Aligments for a candidate for atoB in Pseudomonas fluorescens FW300-N2E2

Align Acetyl-CoA acetyltransferase; Acetoacetyl-CoA thiolase; EC 2.3.1.9 (characterized)
to candidate Pf6N2E2_2835 Beta-ketoadipyl CoA thiolase (EC 2.3.1.-)

Query= SwissProt::Q0AVM3
         (396 letters)



>lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2835 Beta-ketoadipyl CoA
           thiolase (EC 2.3.1.-)
          Length = 400

 Score =  326 bits (836), Expect = 6e-94
 Identities = 177/399 (44%), Positives = 248/399 (62%), Gaps = 9/399 (2%)

Query: 3   REVVLVGACRTPVGTFGGTLKDVGSAQLGAIVMGEAIKR-AGIKAEQIDEVIFGCVLQAG 61
           R+V +  A RTP+G FGG L  V +  L A+ +   ++R   +    +DEV  GC  QAG
Sbjct: 2   RDVYICDAIRTPIGRFGGGLSAVRADDLAAVPIKALMERNPSVDWSAVDEVFLGCANQAG 61

Query: 62  L-GQNVARQCMINAGIPKEVTAFTINKVCGSGLRAVSLAAQVIKAGDADIIMAGGTENMD 120
              +NVAR  ++ AG+P  +   T+N++C SG+ A+  A + I +G+ ++ +AGG E+M 
Sbjct: 62  EDNRNVARMALLLAGLPDSIPGVTLNRLCASGMDAIGTAFRAIASGEMELAIAGGVESMS 121

Query: 121 KAPFILPNARWGYRMSMPKGDLIDEMVWGGLTDVFNGYH----MGITAENINDMYGITRE 176
           +APF++  A   +  SM   D    + W  +  +    +    M  TA+N+ D Y ++R 
Sbjct: 122 RAPFVMGKADAAFSRSMKLEDTT--IGWRFINPLMKAQYGVDAMPQTADNVADDYAVSRA 179

Query: 177 EQDAFGFRSQTLAAQAIESGRFKDEIVPVVIKGKKGDIVFDTDEHPRKSTP-EAMAKLAP 235
           +QDAF  RSQ   A A  +G F +EIV V I  KKG+ V + DEHPR  T  E + KL P
Sbjct: 180 DQDAFALRSQQRTAAAQAAGFFAEEIVAVRIAHKKGETVVEQDEHPRADTTLETLGKLKP 239

Query: 236 AFKKGGSVTAGNASGINDAAAAVIVMSKEKADELGIKPMAKVVSYASGGVDPSVMGLGPI 295
                 +VTAGNASG+ND AAA+I+ S E   + G+ P  KV+  AS GV P VMG+GP+
Sbjct: 240 VNGPDKTVTAGNASGVNDGAAALILASAEAVKKHGLTPRGKVLGMASAGVAPRVMGIGPV 299

Query: 296 PASRKALEKAGLTIDDIDLIEANEAFAAQSIAVARDLGWADKMEKVNVNGGAIAIGHPIG 355
           PA RK  E+ GL + D D+IE NEAFA+Q +AV RDLG AD   +VN NGGAIA+GHP+G
Sbjct: 300 PAVRKLTERLGLAVADFDVIELNEAFASQGLAVLRDLGLADDAPQVNPNGGAIALGHPLG 359

Query: 356 SSGARILVTLLYEMQKRGSKKGLATLCIGGGMGTALIVE 394
            SGAR+++T L+ ++K G KKGLAT+C+G G G AL +E
Sbjct: 360 MSGARLVLTALHHLEKTGGKKGLATMCVGVGQGLALAIE 398


Lambda     K      H
   0.317    0.135    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 436
Number of extensions: 21
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 396
Length of database: 400
Length adjustment: 31
Effective length of query: 365
Effective length of database: 369
Effective search space:   134685
Effective search space used:   134685
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory