Align lysine decarboxylase (EC 4.1.1.18) (characterized)
to candidate Pf6N2E2_2991 decarboxylase
Query= BRENDA::A0A2Z4EVE5 (218 letters) >FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2991 Length = 372 Score = 60.8 bits (146), Expect = 3e-14 Identities = 57/193 (29%), Positives = 93/193 (48%), Gaps = 15/193 (7%) Query: 13 RRICVFCGSSQGKKTSYQEAAIELGKELVSRNIDLVYGGGSIGLMGLVSQAVHDG-GR-H 70 R++ VF + + A ELG L ++ ++ G G G + A H+G GR H Sbjct: 85 RKVTVFGSARTPIEHPLYGLARELGAALARSDMMVITGAG-----GGIMAAAHEGAGRDH 139 Query: 71 VIGVIPKTLMPRELTGETVGEVKAVADMH---QRKAEMARHSDAFIALPGGYGTLEELLE 127 +G TL + TV + H RK + +DA + PGG+GTL+E LE Sbjct: 140 SLG-FNITLPFEQHANPTVEGTPNLLPFHFFFTRKLFFVKEADALVLCPGGFGTLDEALE 198 Query: 128 VITWAQLGIHD-KPVGLLNVDG--YYNSLLSFIDKAVEEG-FISPSARHIIVSAPTAKEL 183 V+T Q G PV LL+V G ++ L+FI + +EE +I P+ ++ A+E Sbjct: 199 VLTLVQTGKSPLVPVVLLDVPGGKFWQGALNFIREQLEENRYILPTDMKLMRLVYNAEEA 258 Query: 184 VKKLEEYVPSHES 196 V+++ ++ + S Sbjct: 259 VEEINQFYRNFHS 271 Lambda K H 0.316 0.134 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 188 Number of extensions: 16 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 218 Length of database: 372 Length adjustment: 26 Effective length of query: 192 Effective length of database: 346 Effective search space: 66432 Effective search space used: 66432 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory