Align ABC transporter for L-Lysine, permease component 2 (characterized)
to candidate Pf6N2E2_3920 Cystine ABC transporter, permease protein
Query= reanno::pseudo5_N2C3_1:AO356_09910 (229 letters) >FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3920 Length = 221 Score = 131 bits (329), Expect = 1e-35 Identities = 80/200 (40%), Positives = 115/200 (57%), Gaps = 9/200 (4%) Query: 11 PKLAQGATLTLELVAIAVIAGLLLAIPLGIARSSRLWQVRALPYAYIFFFRGTPLLVQLF 70 P L +GA T+ L + GLLL L + R SR V + Y+ FFRGTPLLVQLF Sbjct: 13 PFLLKGAYYTVFLSLGGMFFGLLLGFGLALMRLSRFKLVNWIARIYVSFFRGTPLLVQLF 72 Query: 71 LVYYGLAQFDAVRSSALWPYLRDPFWCATVTMTLHTAAYIAEILRGAIQAIPKGEIEAAR 130 ++YYGL Q DP A + +L+ AAY EILR AI +I +G+ EAA Sbjct: 73 VIYYGLPQLGIEL---------DPLPAALIGFSLNMAAYACEILRAAISSIERGQWEAAA 123 Query: 131 ALGMSRPKALFYIMLPRAARIGLPAYSNEVILMLKASALASTVTLLELTGMARTIIARTY 190 ++GM+R + L +LP+AAR LP N I ++K +ALA+T+ + EL A+ I ART+ Sbjct: 124 SIGMTRAQTLRRAILPQAARTALPPLGNSFISLVKDTALAATIQVPELFRQAQLITARTF 183 Query: 191 LPVEIFFAAGMFYLLMSFLL 210 ++ AA + Y +++ +L Sbjct: 184 EIFTMYLAAALIYWVLATVL 203 Lambda K H 0.331 0.141 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 139 Number of extensions: 7 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 229 Length of database: 221 Length adjustment: 22 Effective length of query: 207 Effective length of database: 199 Effective search space: 41193 Effective search space used: 41193 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory