Align NADP-dependent mannitol dehydrogenase; MtDH; Mannitol 2-dehydrogenase [NADP(+)]; EC 1.1.1.138 (characterized)
to candidate Pf6N2E2_5206 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100)
Query= SwissProt::O93868 (262 letters) >FitnessBrowser__pseudo6_N2E2:Pf6N2E2_5206 Length = 247 Score = 119 bits (298), Expect = 6e-32 Identities = 78/254 (30%), Positives = 131/254 (51%), Gaps = 20/254 (7%) Query: 12 KTIIVTGGNRGIGLAFTRAVAAAGANVAVIYRSAKDAVEVTEKVGKEFGVKTKAYQCDVS 71 KT ++TGGN GIGLA + A GA VA+ R A ++G GV T DVS Sbjct: 5 KTALITGGNSGIGLATAKVFLAHGARVAITGRDADKLAAARRELGP--GVITLV--ADVS 60 Query: 72 NTDIVTKTIQQIDADLGAISGLIANAGVSVVKPATELTHEDFKFVYDVNVFGVFNTCRAV 131 +++ + + + ++ + G + ANAGV+ P + T E + + D+NV GVF + +A+ Sbjct: 61 SSEQLRQMAKVVEQEFGGLDIFFANAGVAFGTPLSSTTEEQYDRLMDINVKGVFFSVQAI 120 Query: 132 AKLWLQKQQKGSIVVTSSMSSQIINQSSLNGSLTQVFYNSSKAACSNLVKGLAAEWASAG 191 + + GSI++++S +Q+ G+ + ++SKAA + L+AE Sbjct: 121 EPI---MRPGGSIILSTSWLNQV-------GAPDRTLLSASKAAVRCFARTLSAELLERR 170 Query: 192 IRVNALSPGYVNT------DQTAHMDKKIRDHQASNIPLNRFAQPEEMTGQAILLLSDHA 245 IRVNA+SPG + T +Q+ + + + P+ R + EE+ + L SD + Sbjct: 171 IRVNAVSPGAIETPIHHQPNQSEEEYRAYAERVGAQAPIGRMGRAEEVAAAVLFLASDAS 230 Query: 246 TYMTGGEYFIDGGQ 259 +YM G E +DGG+ Sbjct: 231 SYMLGAEVVVDGGR 244 Lambda K H 0.317 0.130 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 151 Number of extensions: 8 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 262 Length of database: 247 Length adjustment: 24 Effective length of query: 238 Effective length of database: 223 Effective search space: 53074 Effective search space used: 53074 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory