GapMind for catabolism of small carbon sources

 

Aligments for a candidate for TT_C0211 in Pseudomonas fluorescens FW300-N2E2

Align Sugar-binding transport ATP-binding protein aka MalK1 aka TT_C0211, component of The trehalose/maltose/sucrose/palatinose porter (TTC1627-9) plus MalK1 (ABC protein, shared with 3.A.1.1.24) (Silva et al. 2005; Chevance et al., 2006). The receptor (TTC1627) binds disaccharide alpha-glycosides, namely trehalose (alpha-1,1), sucrose (alpha-1,2), maltose (alpha-1,4), palatinose (alpha-1,6) and glucose (characterized)
to candidate Pf6N2E2_1649 Maltose/maltodextrin transport ATP-binding protein MalK (EC 3.6.3.19)

Query= TCDB::Q72L52
         (376 letters)



>lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_1649 Maltose/maltodextrin
           transport ATP-binding protein MalK (EC 3.6.3.19)
          Length = 384

 Score =  329 bits (844), Expect = 7e-95
 Identities = 182/367 (49%), Positives = 247/367 (67%), Gaps = 22/367 (5%)

Query: 3   KVRLEHVWKRFGKVVAVKDFNLETEDGEFVVFVGPSGCGKTTTLRMIAGLEEISEGNIYI 62
           K++L++V K+ G    ++D +LE   GEFVVFVGPSGCGK+T LR+IAGL+ I +G++ I
Sbjct: 3   KLKLDNVNKQLGGARILRDVSLEISAGEFVVFVGPSGCGKSTLLRLIAGLDSICDGDLLI 62

Query: 63  GDRLVNDVPPKDRDIAMVFQNYALYPHMNVYENMAFGLRLRRYPKDEIDRRVKEAARILK 122
             R VND+ P++R + MVFQ+YALYPHM+VY+N++FGL+L +  K  +  RV + A+IL+
Sbjct: 63  DGRRVNDLEPRERGVGMVFQSYALYPHMSVYDNISFGLKLAKTEKASLRERVLKTAQILQ 122

Query: 123 IEHLLNRKPRELSGGQRQRVAMGRAIVREPKVFLMDEPLSNLDAKLRVEMRAEIAKLQRR 182
           ++ LL RKPRELSGGQRQRVAMGRA+ REP + L DEPLSNLDA LRV+MR EIA+L  R
Sbjct: 123 LDKLLQRKPRELSGGQRQRVAMGRAMAREPDILLFDEPLSNLDASLRVQMRNEIARLHGR 182

Query: 183 LGVTTIYVTHDQVEAMTLGHRIVVMKDGEIQQVDTPLNLYDFPANRFVAGFIGSPSMNFV 242
           LG T IYVTHDQVEAMTL  +IVV+  G I+QV +P  LY+ PA+RFVAGF+GSP MNF+
Sbjct: 183 LGSTMIYVTHDQVEAMTLADKIVVLNGGRIEQVGSPRELYEHPASRFVAGFLGSPKMNFL 242

Query: 243 RAGVEVQGEKVYLVAPGFRIRANAVLGSALKPY------AGKEVWLGVRPEHLGLKGYTT 296
            A +   GE   + +P        +LG    P+       G  + LG+RPEH+ LK    
Sbjct: 243 PARLHSPGETSQIDSP--------LLGMTPLPFDSAHLAVGSPLTLGIRPEHMSLKA--- 291

Query: 297 IPEEENVLRGEVEV--VEPLGAETEIHV-AVNGTLLVAKVDGHAPVKPGDKVELLADTQR 353
              + +   G V V  VE LG+ET +H+ +     L+ + + +A  + GD+VEL      
Sbjct: 292 --AQGSAGVGVVGVVGVEYLGSETYVHLESGEDEPLICRCEVNAGWRVGDRVELQLAFGS 349

Query: 354 LHAFDLE 360
           +H FD +
Sbjct: 350 VHLFDAD 356


Lambda     K      H
   0.320    0.139    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 448
Number of extensions: 23
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 376
Length of database: 384
Length adjustment: 30
Effective length of query: 346
Effective length of database: 354
Effective search space:   122484
Effective search space used:   122484
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory