GapMind for catabolism of small carbon sources

 

Aligments for a candidate for fadB in Pseudomonas fluorescens FW300-N2E2

Align Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 (characterized)
to candidate Pf6N2E2_2290 Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)

Query= SwissProt::P28793
         (715 letters)



>lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2290 Enoyl-CoA hydratase
           (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA
           isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase
           (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC
           5.1.2.3)
          Length = 715

 Score = 1309 bits (3388), Expect = 0.0
 Identities = 666/715 (93%), Positives = 690/715 (96%)

Query: 1   MIYEGKAITVTALESGIVELKFDLKGESVNKFNRLTLNELRQAVDAIKADASVKGVIVSS 60
           MIYEGKAITV ALESGIVELKFDLKGESVNKFNRLTLNELRQAVD IKADAS+KGVIVSS
Sbjct: 1   MIYEGKAITVKALESGIVELKFDLKGESVNKFNRLTLNELRQAVDTIKADASIKGVIVSS 60

Query: 61  GKDVFIVGADITEFVENFKLPDAELIAGNLEANKIFSDFEDLNVPTVAAINGIALGGGLE 120
           GKDVFIVGADITEFV+NFKLPDAELIAGNLEAN+IFSDFEDLNVPTV AINGIALGGGLE
Sbjct: 61  GKDVFIVGADITEFVDNFKLPDAELIAGNLEANRIFSDFEDLNVPTVVAINGIALGGGLE 120

Query: 121 MCLAADFRVMADSAKIGLPEVKLGIYPGFGGTVRLPRLIGVDNAVEWIASGKENRAEDAL 180
           MCLAAD+RVM+ +AKIGLPEVKLGIYPGFGGTVRLPRLIG DNA+EWIA+GKENRAEDAL
Sbjct: 121 MCLAADYRVMSTTAKIGLPEVKLGIYPGFGGTVRLPRLIGADNAIEWIAAGKENRAEDAL 180

Query: 181 KVSAVDAVVTADKLGAAALDLIKRAISGELDYKAKRQPKLEKLKLNAIEQMMAFETAKGF 240
           KV AVDAVV   KL  AAL+ IKRAISGE DYKAKRQPKLEKLKLNAIEQMMAFETAKGF
Sbjct: 181 KVGAVDAVVEPGKLQEAALETIKRAISGEFDYKAKRQPKLEKLKLNAIEQMMAFETAKGF 240

Query: 241 VAGQAGPNYPAPVEAIKTIQKAANFGRDKALEVEAAGFAKLAKTSASNCLIGLFLNDQEL 300
           VAGQAGPNYPAPVEAIKTIQKAA+FGRDKALEVEAAGF KLAKTSA+  LIGLFLNDQEL
Sbjct: 241 VAGQAGPNYPAPVEAIKTIQKAASFGRDKALEVEAAGFVKLAKTSAAQSLIGLFLNDQEL 300

Query: 301 KKKAKVYDKIAKDVKQAAVLGAGIMGGGIAYQSASKGTPILMKDINEHGIEQGLAEAAKL 360
           KKKAK YD+IA+DVKQAAVLGAGIMGGGIAYQSASKGTPILMKDINEHGIEQGLAEAAKL
Sbjct: 301 KKKAKAYDEIARDVKQAAVLGAGIMGGGIAYQSASKGTPILMKDINEHGIEQGLAEAAKL 360

Query: 361 LVGRVDKGRMTPAKMAEVLNGIRPTLSYGDFGNVDLVVEAVVENPKVKQAVLAEVENHVR 420
           LVGRVDKGRMT AKMAEVLNGIRPTLSYGDFG+VDLVVEAVVE+PKVKQAVLAEVE+ V+
Sbjct: 361 LVGRVDKGRMTAAKMAEVLNGIRPTLSYGDFGHVDLVVEAVVESPKVKQAVLAEVEDKVK 420

Query: 421 EDAILASNTSTISISLLAKALKRPENFVGMHFFNPVHMMPLVEVIRGEKSSDLAVATTVA 480
           ED ILASNTSTISISLLAKALKRPENFVGMHFFNPVHMMPLVEVIRGEKSS+ AVATTVA
Sbjct: 421 EDTILASNTSTISISLLAKALKRPENFVGMHFFNPVHMMPLVEVIRGEKSSEHAVATTVA 480

Query: 481 YAKKMGKNPIVVNDCPGFLVNRVLFPYFGGFAKLVSAGVDFVRIDKVMEKFGWPMGPAYL 540
           YAKKMGKNPIVVNDCPGFLVNRVLFPYFGGFAKLVSAGVDFVRIDK+MEKFGWPMGPAYL
Sbjct: 481 YAKKMGKNPIVVNDCPGFLVNRVLFPYFGGFAKLVSAGVDFVRIDKIMEKFGWPMGPAYL 540

Query: 541 MDVVGIDTGHHGRDVMAEGFPDRMKDDRRSAIDALYEAKRLGQKNGKGFYAYEADKKGKQ 600
           MDVVGIDTGHHGRDVMAEGFPDRMKDDRRSA+D LYEAKRLGQKNGKGFYAYE DK+GKQ
Sbjct: 541 MDVVGIDTGHHGRDVMAEGFPDRMKDDRRSAVDVLYEAKRLGQKNGKGFYAYETDKRGKQ 600

Query: 601 KKLVDSSVLEVLKPIVYEQRDVTDEDIINWMMIPLCLETVRCLEDGIVETAAEADMGLVY 660
           KK+ DSSVLEVLKPIVYEQR+VTDEDIINWMMIPLCLETVRCLEDGIVETAAEADMGLVY
Sbjct: 601 KKVADSSVLEVLKPIVYEQREVTDEDIINWMMIPLCLETVRCLEDGIVETAAEADMGLVY 660

Query: 661 GIGFPLFRGGALRYIDSIGVAEFVALADQYAELGALYHPTAKLREMAKNGQSFFG 715
           GIGFP FRGGALRYIDSIGVAEFVALADQYA+LGALYHPTAKLREMAKNGQSFFG
Sbjct: 661 GIGFPPFRGGALRYIDSIGVAEFVALADQYADLGALYHPTAKLREMAKNGQSFFG 715


Lambda     K      H
   0.318    0.137    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1693
Number of extensions: 59
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 715
Length of database: 715
Length adjustment: 39
Effective length of query: 676
Effective length of database: 676
Effective search space:   456976
Effective search space used:   456976
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory